YFCJ_ECOLI
ID YFCJ_ECOLI Reviewed; 392 AA.
AC P77549;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Uncharacterized MFS-type transporter YfcJ {ECO:0000255|HAMAP-Rule:MF_02091, ECO:0000305};
GN Name=yfcJ {ECO:0000255|HAMAP-Rule:MF_02091};
GN OrderedLocusNames=b2322, JW2319;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RA Pease A.J., Schoenlein P.V., Winkler M.E.;
RT "Sequence from fabB to pdxB.";
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [6]
RP OVEREXPRESSION, DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=22952739; DOI=10.1371/journal.pone.0043700;
RA Koita K., Rao C.V.;
RT "Identification and analysis of the putative pentose sugar efflux
RT transporters in Escherichia coli.";
RL PLoS ONE 7:E43700-E43700(2012).
CC -!- FUNCTION: Overexpression has no effect on intracellular arabinose
CC concentrations. {ECO:0000269|PubMed:22952739}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_02091, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_02091}.
CC -!- DISRUPTION PHENOTYPE: Deletion increases intracellular arabinose
CC concentrations as compared to the wild type.
CC {ECO:0000269|PubMed:22952739}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. YfcJ family.
CC {ECO:0000255|HAMAP-Rule:MF_02091, ECO:0000305}.
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DR EMBL; U76961; AAB36528.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75382.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16179.1; -; Genomic_DNA.
DR PIR; H65004; H65004.
DR RefSeq; NP_416825.1; NC_000913.3.
DR RefSeq; WP_000127781.1; NZ_LN832404.1.
DR AlphaFoldDB; P77549; -.
DR SMR; P77549; -.
DR BioGRID; 4260717; 222.
DR BioGRID; 851132; 1.
DR IntAct; P77549; 1.
DR STRING; 511145.b2322; -.
DR TCDB; 2.A.1.46.6; the major facilitator superfamily (mfs).
DR PaxDb; P77549; -.
DR PRIDE; P77549; -.
DR EnsemblBacteria; AAC75382; AAC75382; b2322.
DR EnsemblBacteria; BAA16179; BAA16179; BAA16179.
DR GeneID; 946791; -.
DR KEGG; ecj:JW2319; -.
DR KEGG; eco:b2322; -.
DR PATRIC; fig|1411691.4.peg.4411; -.
DR EchoBASE; EB3866; -.
DR eggNOG; COG2814; Bacteria.
DR HOGENOM; CLU_001265_10_3_6; -.
DR InParanoid; P77549; -.
DR OMA; LFVHQEL; -.
DR PhylomeDB; P77549; -.
DR BioCyc; EcoCyc:B2322-MON; -.
DR PRO; PR:P77549; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1250.20; -; 1.
DR HAMAP; MF_02091; MFS_YfcJ; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR037541; MFS_YfcJ.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..392
FT /note="Uncharacterized MFS-type transporter YfcJ"
FT /id="PRO_0000087801"
FT TOPO_DOM 1..19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..51
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 52..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 83..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..119
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 120..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..148
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 149..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 172..175
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 176..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..209
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 210..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..246
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 247..269
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 270..275
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 276..298
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 299..302
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 303..325
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 326..337
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 338..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..369
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 370..389
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 390..392
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 392 AA; 40465 MW; 0F0E23ABAA9009E4 CRC64;
MTAVSQTETR SSANFSLFRI AFAVFLTYMT VGLPLPVIPL FVHHELGYGN TMVGIAVGIQ
FLATVLTRGY AGRLADQYGA KRSALQGMLA CGLAGGALLL AAILPVSAPF KFALLVVGRL
ILGFGESQLL TGALTWGLGI VGPKHSGKVM SWNGMAIYGA LAVGAPLGLL IHSHYGFAAL
AITTMVLPVL AWACNGTVRK VPALAGERPS LWSVVGLIWK PGLGLALQGV GFAVIGTFVS
LYFASKGWAM AGFTLTAFGG AFVVMRVMFG WMPDRFGGVK VAIVSLLVET VGLLLLWQAP
GAWVALAGAA LTGAGCSLIF PALGVEVVKR VPSQVRGTAL GGYAAFQDIA LGVSGPLAGM
LATTFGYSSV FLAGAISAVL GIIVTILSFR RG
