CBDB_BURCE
ID CBDB_BURCE Reviewed; 165 AA.
AC Q51602; O08067;
DT 01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=2-halobenzoate 1,2-dioxygenase small subunit;
DE EC=1.14.12.13;
DE AltName: Full=2-chlorobenzoate 1,2-dioxygenase;
GN Name=cbdB;
OS Burkholderia cepacia (Pseudomonas cepacia).
OG Plasmid pBAH1.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=2CBS;
RX PubMed=7530709; DOI=10.1128/jb.177.3.667-675.1995;
RA Haak B., Fetzner S., Lingens F.;
RT "Cloning, nucleotide sequence, and expression of the plasmid-encoded genes
RT for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas
RT cepacia 2CBS.";
RL J. Bacteriol. 177:667-675(1995).
RN [2]
RP PROTEIN SEQUENCE OF 2-15, AND CHARACTERIZATION.
RC STRAIN=2CBS;
RX PubMed=1370284; DOI=10.1128/jb.174.1.279-290.1992;
RA Fetzner S., Mueller R., Lingens F.;
RT "Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-
RT component enzyme system from Pseudomonas cepacia 2CBS.";
RL J. Bacteriol. 174:279-290(1992).
CC -!- FUNCTION: Component of 2-halobenzoate dioxygenase multicomponent enzyme
CC system which catalyzes the incorporation of both atoms of molecular
CC oxygen into 2-halobenzoate to form catechol.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2-halobenzoate + H(+) + NADH + O2 = a halide anion +
CC catechol + CO2 + NAD(+); Xref=Rhea:RHEA:53736, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15379, ChEBI:CHEBI:16042, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:18135, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945,
CC ChEBI:CHEBI:70856; EC=1.14.12.13;
CC -!- PATHWAY: Xenobiotic degradation; benzoate degradation via CoA ligation.
CC -!- SUBUNIT: Heterohexamer of 3 large (CbdA) subunits and 3 small (CbdB)
CC subunits. The heterohexamer is part of 2-halobenzoate dioxygenase two
CC component enzyme system. The other component is a NADH:acceptor
CC reductase (CdbC).
CC -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC beta subunit family. {ECO:0000305}.
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DR EMBL; X79076; CAA55682.1; -; Genomic_DNA.
DR AlphaFoldDB; Q51602; -.
DR SMR; Q51602; -.
DR KEGG; ag:CAA55682; -.
DR BioCyc; MetaCyc:MON-14764; -.
DR UniPathway; UPA00233; -.
DR GO; GO:0018626; F:2-chlorobenzoate 1,2-dioxygenase activity; IEA:UniProtKB-EC.
DR GO; GO:0010128; P:benzoate catabolic process via CoA ligation; IEA:UniProtKB-UniPathway.
DR CDD; cd00667; ring_hydroxylating_dioxygenases_beta; 1.
DR InterPro; IPR017641; Benzo_1-2-diOase_ssu.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR000391; Rng_hydr_dOase-bsu.
DR PANTHER; PTHR41534; PTHR41534; 1.
DR Pfam; PF00866; Ring_hydroxyl_B; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
DR TIGRFAMs; TIGR03232; benzo_1_2_benB; 1.
PE 1: Evidence at protein level;
KW Aromatic hydrocarbons catabolism; Dioxygenase; Direct protein sequencing;
KW NAD; Oxidoreductase; Plasmid.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:1370284"
FT CHAIN 2..165
FT /note="2-halobenzoate 1,2-dioxygenase small subunit"
FT /id="PRO_0000085071"
FT CONFLICT 12
FT /note="V -> C (in Ref. 1; CAA55682)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 165 AA; 19632 MW; 4886AF20AE127AA8 CRC64;
MTSLESSYLD VVAFIFREAR LLDDRSWDEW LECYDPEAVF WMPCWDDADT LVDDPRKHVS
LIYYSDRMGL EDRVFRLRSE RSGASTPEPR TTHNIANVEI LERTERQIEA RFNWHTMNYR
YKLLDHYFGT SFYTLKVSSS GLSILNKKVV LKNDLIHQVI DVYHV
