ID   YFI3_ECO57              Reviewed;         153 AA.
AC   Q7BSX4; O82923;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Uncharacterized endonuclease;
DE            EC=3.1.-.-;
DE   Flags: Precursor;
GN   OrderedLocusNames=L7003, ECO57PM65;
OS   Escherichia coli O157:H7.
OG   Plasmid pO157.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=9722640; DOI=10.1093/nar/26.18.4196;
RA   Burland V., Shao Y., Perna N.T., Plunkett G. III, Sofia H.J.,
RA   Blattner F.R.;
RT   "The complete DNA sequence and analysis of the large virulence plasmid of
RT   Escherichia coli O157:H7.";
RL   Nucleic Acids Res. 26:4196-4204(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=9628576; DOI=10.1093/dnares/5.1.1;
RA   Makino K., Ishii K., Yasunaga T., Hattori M., Yokoyama K., Yatsudo H.C.,
RA   Kubota Y., Yamaichi Y., Iida T., Yamamoto K., Honda T., Han C.G.,
RA   Ohtsubo A., Kasamatsu M., Hayashi T., Kuhara S., Shinagawa H.;
RT   "Complete nucleotide sequences of 93-kb and 3.3-kb plasmids of an
RT   enterohemorrhagic Escherichia coli O157:H7 derived from Sakai outbreak.";
RL   DNA Res. 5:1-9(1998).
CC   -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00272}.
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DR   EMBL; AF074613; AAC70071.1; -; Genomic_DNA.
DR   EMBL; AB011549; BAA31822.1; -; Genomic_DNA.
DR   PIR; T00303; T00303.
DR   RefSeq; NP_052672.1; NC_002128.1.
DR   RefSeq; WP_001233853.1; NZ_SWKA01000002.1.
DR   AlphaFoldDB; Q7BSX4; -.
DR   SMR; Q7BSX4; -.
DR   STRING; 155864.EDL933_p0003; -.
DR   EnsemblBacteria; AAC70071; AAC70071; Z_L7003.
DR   EnsemblBacteria; BAA31822; BAA31822; BAA31822.
DR   GeneID; 1789676; -.
DR   KEGG; ece:Z_L7003; -.
DR   KEGG; ecs:pO157p65; -.
DR   PATRIC; fig|386585.9.peg.75; -.
DR   eggNOG; COG1525; Bacteria.
DR   HOGENOM; CLU_046484_7_3_6; -.
DR   Proteomes; UP000000558; Plasmid pO157.
DR   Proteomes; UP000002519; Plasmid pO157.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 2.40.50.90; -; 1.
DR   InterPro; IPR035437; SNase_OB-fold_sf.
DR   InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR   InterPro; IPR002071; Thermonucl_AS.
DR   Pfam; PF00565; SNase; 1.
DR   SMART; SM00318; SNc; 1.
DR   SUPFAM; SSF50199; SSF50199; 1.
DR   PROSITE; PS01123; TNASE_1; 1.
DR   PROSITE; PS01284; TNASE_2; 1.
DR   PROSITE; PS50830; TNASE_3; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..153
FT                   /note="Uncharacterized endonuclease"
FT                   /id="PRO_0000034396"
FT   ACT_SITE        46
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        88
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   153 AA;  17872 MW;  635483EEA027D090 CRC64;
     MRKYIPLVLF IFSWPVLCAD IHGRVVRVLD GDTIEVMDSR KAVRIRLVNI DAPEKKQDYG
     RWSTDMMKSL VAGKTVTVTY FQRDRYGRML GQVYAPDGMN VNQFMVRAGA AWVYEQYNTD
     PVLPVLQNEA RQQKRGLWSD ADPVPPWIWR HRK