ID   YFI3_ECOLX              Reviewed;         153 AA.
AC   Q99342;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Uncharacterized endonuclease;
DE            EC=3.1.-.-;
DE   AltName: Full=ORFA;
DE   Flags: Precursor;
OS   Escherichia coli.
OG   Plasmid IncFII R100 (NR1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2164585; DOI=10.1016/0022-2836(90)90145-c;
RA   Yoshioka Y., Fujita Y., Ohtsubo E.;
RT   "Nucleotide sequence of the promoter-distal region of the tra operon of
RT   plasmid R100, including traI (DNA helicase I) and traD genes.";
RL   J. Mol. Biol. 214:39-53(1990).
CC   -!- SIMILARITY: Belongs to the thermonuclease family. {ECO:0000255|PROSITE-
CC       ProRule:PRU00272}.
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DR   EMBL; X55815; CAA39343.1; -; Genomic_DNA.
DR   PIR; S10665; S10665.
DR   RefSeq; WP_001233850.1; NZ_WVVH01000037.1.
DR   RefSeq; YP_001096506.1; NC_009133.1.
DR   AlphaFoldDB; Q99342; -.
DR   SMR; Q99342; -.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   Gene3D; 2.40.50.90; -; 1.
DR   InterPro; IPR035437; SNase_OB-fold_sf.
DR   InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR   InterPro; IPR002071; Thermonucl_AS.
DR   Pfam; PF00565; SNase; 1.
DR   SMART; SM00318; SNc; 1.
DR   SUPFAM; SSF50199; SSF50199; 1.
DR   PROSITE; PS01123; TNASE_1; 1.
DR   PROSITE; PS01284; TNASE_2; 1.
DR   PROSITE; PS50830; TNASE_3; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Nuclease; Plasmid; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..153
FT                   /note="Uncharacterized endonuclease"
FT                   /id="PRO_0000034395"
FT   ACT_SITE        46
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        54
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        88
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   153 AA;  17854 MW;  659243E866E7D090 CRC64;
     MRKYIPLVLF IFSWPVLCAD IHGRVVRVLD GDTIEVMDSR KAVRIRLVNI DAPEKKQDYG
     RWSTDMMKSL VAGKTVTVTY FQRDRYGRIL GQVYAPDGMN VNQFMVRAGA AWVYEQYNTD
     PVLPVLQNEA RQQKRGLWSD ADPVPPWIWR HRK