ID   YFIF_ECO57              Reviewed;         345 AA.
AC   P0AGJ6; P33635; P76999;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Uncharacterized tRNA/rRNA methyltransferase YfiF;
DE            EC=2.1.1.-;
GN   Name=yfiF; OrderedLocusNames=Z3865, ECs3447;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC       superfamily. RNA methyltransferase TrmH family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG57697.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB36870.1; -; Genomic_DNA.
DR   PIR; G91059; G91059.
DR   RefSeq; NP_311474.1; NC_002695.1.
DR   RefSeq; WP_000997403.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AGJ6; -.
DR   SMR; P0AGJ6; -.
DR   IntAct; P0AGJ6; 1.
DR   MINT; P0AGJ6; -.
DR   STRING; 155864.EDL933_3746; -.
DR   PRIDE; P0AGJ6; -.
DR   EnsemblBacteria; AAG57697; AAG57697; Z3865.
DR   EnsemblBacteria; BAB36870; BAB36870; ECs_3447.
DR   GeneID; 914879; -.
DR   KEGG; ece:Z3865; -.
DR   KEGG; ecs:ECs_3447; -.
DR   PATRIC; fig|386585.9.peg.3602; -.
DR   eggNOG; COG0566; Bacteria.
DR   HOGENOM; CLU_021322_2_1_6; -.
DR   OMA; NRAEEMK; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   Gene3D; 3.30.1330.30; -; 1.
DR   Gene3D; 3.40.1280.10; -; 1.
DR   InterPro; IPR029028; Alpha/beta_knot_MTases.
DR   InterPro; IPR029064; L30e-like.
DR   InterPro; IPR004441; rRNA_MeTrfase_TrmH.
DR   InterPro; IPR001537; SpoU_MeTrfase.
DR   InterPro; IPR013123; SpoU_subst-bd.
DR   InterPro; IPR029026; tRNA_m1G_MTases_N.
DR   InterPro; IPR016479; YfiF_prd.
DR   PANTHER; PTHR46429; PTHR46429; 1.
DR   PANTHER; PTHR46429:SF2; PTHR46429:SF2; 1.
DR   Pfam; PF00588; SpoU_methylase; 1.
DR   Pfam; PF08032; SpoU_sub_bind; 1.
DR   PIRSF; PIRSF006280; YfiF_prd; 1.
DR   SMART; SM00967; SpoU_sub_bind; 1.
DR   SUPFAM; SSF55315; SSF55315; 1.
DR   SUPFAM; SSF75217; SSF75217; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..345
FT                   /note="Uncharacterized tRNA/rRNA methyltransferase YfiF"
FT                   /id="PRO_0000159815"
FT   REGION          1..98
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..31
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        41..69
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   345 AA;  37784 MW;  9845E9884D8369BF CRC64;
     MNDEMKGKSG KVKVMYVRSD DDSDKRTHNP RTGKGGGRPG KSRADGGRRP ARDDKQSQPR
     DRKWEDSPWR TVSRAPGDET PEKADHGGIS GKSFIDPEVL RRQRAEETRV YGENACQALF
     QSRPEAIVRA WFIQSVTPRF KEALRWMAAN RKAYHVVDEA ELTKASGTEH HGGVCFLIKK
     RNGTTVQQWV SQAGAQDCVL ALENESNPHN LGGMMRSCAH FGVKGVVVQD AALLESGAAI
     RTAEGGAEHV QPITGDNIVN VLDDFRQAGY TVVTTSSEQG KPLFKTSLPA KMVLVLGQEY
     EGLPDAARDP NDLRVKIDGT GNVAGLNISV ATGVLLGEWW RQNKA