YFIF_ECOLI
ID YFIF_ECOLI Reviewed; 345 AA.
AC P0AGJ5; P33635; P76999;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Uncharacterized tRNA/rRNA methyltransferase YfiF;
DE EC=2.1.1.-;
GN Name=yfiF; OrderedLocusNames=b2581, JW2565;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Nashimoto H.;
RT "Non-ribosomal proteins affecting the assembly of ribosomes in Escherichia
RT coli.";
RL (In) Nierhaus K.H. (eds.);
RL The translational apparatus, pp.185-195, Plenum Press, New York (1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 268-345.
RX PubMed=2836397; DOI=10.1016/s0021-9258(18)68566-7;
RA Varshney U., Hutcheon T., de Sande J.H.;
RT "Sequence analysis, expression, and conservation of Escherichia coli uracil
RT DNA glycosylase and its gene (ung).";
RL J. Biol. Chem. 263:7776-7784(1988).
RN [6]
RP IDENTIFICATION.
RX PubMed=8265370; DOI=10.1093/nar/21.23.5519;
RA Koonin E.V., Rudd K.E.;
RT "SpoU protein of Escherichia coli belongs to a new family of putative rRNA
RT methylases.";
RL Nucleic Acids Res. 21:5519-5519(1993).
RN [7]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
CC -!- INTERACTION:
CC P0AGJ5; P17443: murG; NbExp=3; IntAct=EBI-547368, EBI-554881;
CC -!- SIMILARITY: Belongs to the class IV-like SAM-binding methyltransferase
CC superfamily. RNA methyltransferase TrmH family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=D13169; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; D13169; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U00096; AAC75634.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16467.2; -; Genomic_DNA.
DR EMBL; J03725; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; D65036; D65036.
DR RefSeq; NP_417076.1; NC_000913.3.
DR RefSeq; WP_000997403.1; NZ_STEB01000011.1.
DR AlphaFoldDB; P0AGJ5; -.
DR SMR; P0AGJ5; -.
DR BioGRID; 4263473; 35.
DR BioGRID; 851403; 1.
DR DIP; DIP-47835N; -.
DR IntAct; P0AGJ5; 79.
DR STRING; 511145.b2581; -.
DR jPOST; P0AGJ5; -.
DR PaxDb; P0AGJ5; -.
DR PRIDE; P0AGJ5; -.
DR DNASU; 947066; -.
DR EnsemblBacteria; AAC75634; AAC75634; b2581.
DR EnsemblBacteria; BAA16467; BAA16467; BAA16467.
DR GeneID; 947066; -.
DR KEGG; ecj:JW2565; -.
DR KEGG; eco:b2581; -.
DR PATRIC; fig|1411691.4.peg.4153; -.
DR EchoBASE; EB1734; -.
DR eggNOG; COG0566; Bacteria.
DR HOGENOM; CLU_021322_2_1_6; -.
DR InParanoid; P0AGJ5; -.
DR OMA; NRAEEMK; -.
DR PhylomeDB; P0AGJ5; -.
DR BioCyc; EcoCyc:EG11786-MON; -.
DR PRO; PR:P0AGJ5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0008173; F:RNA methyltransferase activity; IBA:GO_Central.
DR GO; GO:0001510; P:RNA methylation; IBA:GO_Central.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR Gene3D; 3.30.1330.30; -; 1.
DR Gene3D; 3.40.1280.10; -; 1.
DR InterPro; IPR029028; Alpha/beta_knot_MTases.
DR InterPro; IPR029064; L30e-like.
DR InterPro; IPR004441; rRNA_MeTrfase_TrmH.
DR InterPro; IPR001537; SpoU_MeTrfase.
DR InterPro; IPR013123; SpoU_subst-bd.
DR InterPro; IPR029026; tRNA_m1G_MTases_N.
DR InterPro; IPR016479; YfiF_prd.
DR PANTHER; PTHR46429; PTHR46429; 1.
DR PANTHER; PTHR46429:SF2; PTHR46429:SF2; 1.
DR Pfam; PF00588; SpoU_methylase; 1.
DR Pfam; PF08032; SpoU_sub_bind; 1.
DR PIRSF; PIRSF006280; YfiF_prd; 1.
DR SMART; SM00967; SpoU_sub_bind; 1.
DR SUPFAM; SSF55315; SSF55315; 1.
DR SUPFAM; SSF75217; SSF75217; 1.
PE 1: Evidence at protein level;
KW Methyltransferase; Reference proteome; Transferase.
FT CHAIN 1..345
FT /note="Uncharacterized tRNA/rRNA methyltransferase YfiF"
FT /id="PRO_0000159814"
FT REGION 1..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..31
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..69
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 345 AA; 37784 MW; 9845E9884D8369BF CRC64;
MNDEMKGKSG KVKVMYVRSD DDSDKRTHNP RTGKGGGRPG KSRADGGRRP ARDDKQSQPR
DRKWEDSPWR TVSRAPGDET PEKADHGGIS GKSFIDPEVL RRQRAEETRV YGENACQALF
QSRPEAIVRA WFIQSVTPRF KEALRWMAAN RKAYHVVDEA ELTKASGTEH HGGVCFLIKK
RNGTTVQQWV SQAGAQDCVL ALENESNPHN LGGMMRSCAH FGVKGVVVQD AALLESGAAI
RTAEGGAEHV QPITGDNIVN VLDDFRQAGY TVVTTSSEQG KPLFKTSLPA KMVLVLGQEY
EGLPDAARDP NDLRVKIDGT GNVAGLNISV ATGVLLGEWW RQNKA
