YFIT_BACSU
ID YFIT_BACSU Reviewed; 178 AA.
AC O31562; Q79EW7;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Putative metal-dependent hydrolase YfiT;
DE EC=3.-.-.-;
GN Name=yfiT; OrderedLocusNames=BSU08390;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8946165; DOI=10.1093/dnares/3.4.257;
RA Yamamoto H., Uchiyama S., Sekiguchi J.;
RT "Cloning and sequencing of a 27.8-kb nucleotide sequence of the 79 degrees-
RT 81 degrees region of the Bacillus subtilis genome containing the sspE
RT locus.";
RL DNA Res. 3:257-262(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS), SUBUNIT, AND COFACTOR.
RX PubMed=15581359; DOI=10.1021/bi048665r;
RA Rajan S.S., Yang X., Shuvalova L., Collart F., Anderson W.F.;
RT "YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with
RT an unusual four-helix bundle topology.";
RL Biochemistry 43:15472-15479(2004).
CC -!- FUNCTION: Possible metal-dependent hydrolase.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000269|PubMed:15581359};
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000269|PubMed:15581359};
CC Note=Binds 1 zinc ion per subunit. Is able to bind nickel instead of
CC zinc. {ECO:0000269|PubMed:15581359};
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:15581359}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the metal hydrolase YfiT family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D85082; BAA24460.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12668.1; -; Genomic_DNA.
DR PIR; F69804; F69804.
DR RefSeq; NP_388720.1; NC_000964.3.
DR RefSeq; WP_003243944.1; NZ_JNCM01000032.1.
DR PDB; 1RXQ; X-ray; 1.70 A; A/B/C/D=1-178.
DR PDBsum; 1RXQ; -.
DR AlphaFoldDB; O31562; -.
DR SMR; O31562; -.
DR STRING; 224308.BSU08390; -.
DR PaxDb; O31562; -.
DR PRIDE; O31562; -.
DR EnsemblBacteria; CAB12668; CAB12668; BSU_08390.
DR GeneID; 939711; -.
DR KEGG; bsu:BSU08390; -.
DR PATRIC; fig|224308.179.peg.907; -.
DR eggNOG; COG2318; Bacteria.
DR InParanoid; O31562; -.
DR OMA; GWTIRQV; -.
DR PhylomeDB; O31562; -.
DR BioCyc; BSUB:BSU08390-MON; -.
DR EvolutionaryTrace; O31562; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.120.450; -; 1.
DR HAMAP; MF_01256; YfiT_hydrol; 1.
DR InterPro; IPR024775; DinB-like.
DR InterPro; IPR034660; DinB/YfiT-like.
DR InterPro; IPR023774; Put_metal_dep_hydrolase_YfiT.
DR Pfam; PF12867; DinB_2; 1.
DR SUPFAM; SSF109854; SSF109854; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Hydrolase; Metal-binding; Nickel;
KW Reference proteome; Zinc.
FT CHAIN 1..178
FT /note="Putative metal-dependent hydrolase YfiT"
FT /id="PRO_0000162373"
FT BINDING 67
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 160
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT BINDING 164
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT HELIX 21..42
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 47..50
FT /evidence="ECO:0007829|PDB:1RXQ"
FT STRAND 52..55
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 61..84
FT /evidence="ECO:0007829|PDB:1RXQ"
FT STRAND 85..87
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 95..100
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 102..106
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 109..129
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 132..135
FT /evidence="ECO:0007829|PDB:1RXQ"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:1RXQ"
FT TURN 142..144
FT /evidence="ECO:0007829|PDB:1RXQ"
FT STRAND 147..149
FT /evidence="ECO:0007829|PDB:1RXQ"
FT HELIX 150..175
FT /evidence="ECO:0007829|PDB:1RXQ"
SQ SEQUENCE 178 AA; 20666 MW; 977ECAC0B6C91DD2 CRC64;
MTSVNLSYPI GEYKPRESIS KEQKDKWIQV LEEVPAKLKQ AVEVMTDSQL DTPYRDGGWT
VRQVVHHLAD SHMNSYIRFK LSLTEETPAI RPYDEKAWSE LKDSKTADPS GSLALLQELH
GRWTALLRTL TDQQFKRGFY HPDTKEIITL ENALGLYVWH SHHHIAHITE LSRRMGWS
