CBF1_CANAL
ID CBF1_CANAL Reviewed; 251 AA.
AC Q5A1E3; A0A1D8PML4;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2017, sequence version 2.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Transcriptional regulator CBF1;
GN Name=CBF1; OrderedLocusNames=CAALFM_C406580WA;
GN ORFNames=CaO19.10394, CaO19.2876;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP IDENTIFICATION, AND FUNCTION.
RX PubMed=11481675; DOI=10.1002/yea.757;
RA Eck R., Stoyan T., Kunkel W.;
RT "The centromere-binding factor Cbf1p from Candida albicans complements the
RT methionine auxotrophic phenotype of Saccharomyces cerevisiae.";
RL Yeast 18:1047-1052(2001).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=14659878; DOI=10.1016/j.gene.2003.09.005;
RA Biswas K., Rieger K.J., Morschhauser J.;
RT "Functional characterization of CaCBF1, the Candida albicans homolog of
RT centromere binding factor 1.";
RL Gene 323:43-55(2003).
RN [6]
RP FUNCTION, AND DNA-BINDING.
RX PubMed=18342603; DOI=10.1016/j.molcel.2008.02.006;
RA Hogues H., Lavoie H., Sellam A., Mangos M., Roemer T., Purisima E.,
RA Nantel A., Whiteway M.;
RT "Transcription factor substitution during the evolution of fungal ribosome
RT regulation.";
RL Mol. Cell 29:552-562(2008).
RN [7]
RP INDUCTION.
RX PubMed=22265407; DOI=10.1016/j.cell.2011.10.048;
RA Nobile C.J., Fox E.P., Nett J.E., Sorrells T.R., Mitrovich Q.M.,
RA Hernday A.D., Tuch B.B., Andes D.R., Johnson A.D.;
RT "A recently evolved transcriptional network controls biofilm development in
RT Candida albicans.";
RL Cell 148:126-138(2012).
CC -!- FUNCTION: Transcription factor that binds ribosomal protein gene
CC promoters and rDNA locus with TBF1. Necessary for the expression of
CC genes involved in assimilation of inorganic sulfate. Also required for
CC the expression of respiratory genes and glycolytic genes. Does not bind
CC to centromeres and is not necessary for efficient chromosome
CC segregationas as does S.cerevisiae CBF1. {ECO:0000269|PubMed:11481675,
CC ECO:0000269|PubMed:14659878, ECO:0000269|PubMed:18342603}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P17106}.
CC -!- INDUCTION: Repressed during biofilm formation.
CC {ECO:0000269|PubMed:22265407}.
CC -!- DISRUPTION PHENOTYPE: Leads to auxotrophy for sulfur amino acids.
CC {ECO:0000269|PubMed:14659878}.
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DR EMBL; CP017626; AOW29381.1; -; Genomic_DNA.
DR RefSeq; XP_019330942.1; XM_019475397.1.
DR AlphaFoldDB; Q5A1E3; -.
DR SMR; Q5A1E3; -.
DR BioGRID; 1225818; 1.
DR STRING; 237561.Q5A1E3; -.
DR PRIDE; Q5A1E3; -.
DR GeneID; 3642779; -.
DR KEGG; cal:CAALFM_C406580WA; -.
DR CGD; CAL0000186329; CBF1.
DR VEuPathDB; FungiDB:C4_06580W_A; -.
DR eggNOG; KOG1318; Eukaryota.
DR HOGENOM; CLU_046871_2_0_1; -.
DR InParanoid; Q5A1E3; -.
DR OMA; QSSHQEI; -.
DR OrthoDB; 1471388at2759; -.
DR PRO; PR:Q5A1E3; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Coiled coil; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..251
FT /note="Transcriptional regulator CBF1"
FT /id="PRO_0000426067"
FT DOMAIN 152..200
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 190..223
FT /evidence="ECO:0000255"
FT COMPBIAS 1..33
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 36..74
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..100
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 101..141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..169
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 251 AA; 29153 MW; 5479B57AE244BD24 CRC64;
MVKSHKRTLE KDEEHQEKKK ANKISKDDME IDAELLTQQA SDSAHTDTAT AAVAAVNNEQ
GKELEQTESS TNQTSALDKD DKETKDNLNP REETQSSHQE IDIPKDQLTN QQNLADQHQQ
YQYHQQLAQT NFKTEPTNSA KPPHGSEEWH RQRRENHKEV ERKRRESINT GIRELARLIP
TTDTNKAQIL QRAVEYIKRL KENENNNIEK WTLEKLLTEQ AVSELSASNE KLKHELESAY
REIEQLKRGK K
