ID   YFJS_ECOLI              Reviewed;         147 AA.
AC   O52982; Q2MAD5;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-1998, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Lipoprotein YfjS;
DE   Flags: Precursor;
GN   Name=yfjS; OrderedLocusNames=b2636, JW5921;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF 60-GLY-PHE-61 AND
RP   ARG-136.
RC   STRAIN=K12 / MC4100 / ATCC 35695 / DSM 6574;
RX   PubMed=15252048; DOI=10.1074/jbc.m406390200;
RA   Miyadai H., Tanaka-Masuda K., Matsuyama S., Tokuda H.;
RT   "Effects of lipoprotein overproduction on the induction of DegP (HtrA)
RT   involved in quality control in the Escherichia coli periplasm.";
RL   J. Biol. Chem. 279:39807-39813(2004).
CC   -!- FUNCTION: Does not induce degP when overexpressed unless it is mutated
CC       to resemble YafY. {ECO:0000269|PubMed:15252048}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000305|PubMed:15252048}; Lipid-anchor
CC       {ECO:0000305|PubMed:15252048}.
CC   -!- SIMILARITY: To E.coli YafY. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA79804.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U36840; AAA79804.1; ALT_INIT; Genomic_DNA.
DR   EMBL; U00096; AAC75684.2; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76771.1; -; Genomic_DNA.
DR   PIR; F65042; F65042.
DR   RefSeq; NP_417124.4; NC_000913.3.
DR   RefSeq; WP_000824222.1; NZ_LN832404.1.
DR   AlphaFoldDB; O52982; -.
DR   BioGRID; 4260628; 8.
DR   STRING; 511145.b2636; -.
DR   PaxDb; O52982; -.
DR   PRIDE; O52982; -.
DR   EnsemblBacteria; AAC75684; AAC75684; b2636.
DR   EnsemblBacteria; BAE76771; BAE76771; BAE76771.
DR   GeneID; 945668; -.
DR   KEGG; ecj:JW5921; -.
DR   KEGG; eco:b2636; -.
DR   PATRIC; fig|511145.12.peg.2730; -.
DR   eggNOG; ENOG5032TZC; Bacteria.
DR   HOGENOM; CLU_149187_0_0_6; -.
DR   OMA; SHKSDIA; -.
DR   BioCyc; EcoCyc:G7371-MON; -.
DR   PRO; PR:O52982; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           21..147
FT                   /note="Lipoprotein YfjS"
FT                   /id="PRO_0000018047"
FT   LIPID           21
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           21
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   MUTAGEN         60..61
FT                   /note="GF->DY: 3-fold improved induction of degP upon
FT                   overexpression; when associated with Q-136."
FT                   /evidence="ECO:0000269|PubMed:15252048"
FT   MUTAGEN         136
FT                   /note="R->Q: 3-fold improved induction of degP upon
FT                   overexpression; when associated with 60-D-Y-61."
FT                   /evidence="ECO:0000269|PubMed:15252048"
SQ   SEQUENCE   147 AA;  16735 MW;  294131E411B78C7A CRC64;
     MKRKTLPLLA LVATSLFLSA CDDRSDDLKA ISKFKDLTPP RFSDVVSRQD DVSEEWSQVG
     FSSGLTLQVL RTRESPDGCE GGSYYYLVDM EEKTVQPLMN ALCIADNIKL EYHEVTDPYT
     KEKYFEYSHD GKLMGRLLIP SNPDNRE