ID   YFKA_BACSU              Reviewed;         373 AA.
AC   O34400; C0SP88; O34868; Q79EY5; Q79EY6;
DT   13-OCT-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Putative protein YfkA;
GN   Name=yfkA; Synonyms=ykfB; OrderedLocusNames=BSU07955;
GN   ORFNames=BSU07950, BSU07960;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / AC327;
RX   PubMed=8969503; DOI=10.1099/13500872-142-11-3057;
RA   Yamamoto H., Uchiyama S., Sekiguchi J.;
RT   "Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees
RT   region of the Bacillus subtilis chromosome containing genes for trehalose
RT   metabolism and acetoin utilization.";
RL   Microbiology 142:3057-3065(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305};
CC   -!- SIMILARITY: Belongs to the radical SAM superfamily. {ECO:0000305}.
CC   -!- CAUTION: Was originally (PubMed:8969503, PubMed:9384377) predicted to
CC       be the product of two different genes yfkA and yfkB.
CC       {ECO:0000305|PubMed:8969503, ECO:0000305|PubMed:9384377}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA23391.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=BAA23392.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=BAA23392.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; D83967; BAA23391.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; D83967; BAA23392.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AL009126; CAB12625.2; -; Genomic_DNA.
DR   PIR; F69807; F69807.
DR   RefSeq; NP_388677.2; NC_000964.3.
DR   RefSeq; WP_003233655.1; NZ_JNCM01000032.1.
DR   AlphaFoldDB; O34400; -.
DR   STRING; 224308.BSU07955; -.
DR   jPOST; O34400; -.
DR   PRIDE; O34400; -.
DR   EnsemblBacteria; CAB12625; CAB12625; BSU_07955.
DR   GeneID; 938812; -.
DR   KEGG; bsu:BSU07955; -.
DR   PATRIC; fig|224308.179.peg.861; -.
DR   eggNOG; COG0535; Bacteria.
DR   OMA; ETMINKR; -.
DR   PhylomeDB; O34400; -.
DR   BioCyc; BSUB:BSU07955-MON; -.
DR   BRENDA; 5.1.1.20; 658.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR031004; rSAM_YfkAB.
DR   InterPro; IPR014866; YfkB.
DR   PANTHER; PTHR42836:SF2; PTHR42836:SF2; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08756; YfkB; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01097; Uncharacterised_Radical_SAM_Su; 1.
DR   TIGRFAMs; TIGR04478; rSAM_YfkAB; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..373
FT                   /note="Putative protein YfkA"
FT                   /id="PRO_0000386529"
FT   DOMAIN          26..256
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   BINDING         42
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         46
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         49
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   373 AA;  43257 MW;  8D8BB873422CB98B CRC64;
     MTQNTKLRPI TPEFDPWEAY MDVDQYGDMQ LTNVEFTTTT LCNMRCEHCA VGYTLQPKDP
     NALPIDLLLK RLEEIPRLRS ISITGGEPML SLKSVKEYVV PLLKYAHERG VRTQINSNLT
     LDIERYEWII PYLDVLHISH NWGTVEDFAE IGFAMMDRKP TFEQRARYFE KMIENSRTLV
     DAGVMVSAET MLNKRTLPHI EHIHRQITED MKCQRHEVHP MYPSDFASAL ESLSLKEMRQ
     AIHRLLDIRD ENTWMLFGTL PFYACSPDED DQKLLRRLRA AKNVTVRNDP DGRSRLNVNI
     FDGNIIVTDF GDTPPLGNIQ TDSLPSAYEK WRETKLAKEL SCHCPNVRCL GPNVLVKNSY
     YQDTDFTKRQ ARS