ID   YFKC_BACSU              Reviewed;         280 AA.
AC   O35043; Q79EY4;
DT   24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Uncharacterized MscS family protein YfkC;
GN   Name=yfkC; OrderedLocusNames=BSU07940;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / AC327;
RX   PubMed=8969503; DOI=10.1099/13500872-142-11-3057;
RA   Yamamoto H., Uchiyama S., Sekiguchi J.;
RT   "Cloning and sequencing of a 40.6 kb segment in the 73 degrees-76 degrees
RT   region of the Bacillus subtilis chromosome containing genes for trehalose
RT   metabolism and acetoin utilization.";
RL   Microbiology 142:3057-3065(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=168 / JH642;
RX   PubMed=18310427; DOI=10.1128/aem.01573-07;
RA   Hoffmann T., Boiangiu C., Moses S., Bremer E.;
RT   "Responses of Bacillus subtilis to hypotonic challenges: physiological
RT   contributions of mechanosensitive channels to cellular survival.";
RL   Appl. Environ. Microbiol. 74:2454-2460(2008).
RN   [4]
RP   FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=168 / PS832;
RX   PubMed=17665170; DOI=10.1007/s00203-007-0292-z;
RA   Wahome P.G., Setlow P.;
RT   "Growth, osmotic downshock resistance and differentiation of Bacillus
RT   subtilis strains lacking mechanosensitive channels.";
RL   Arch. Microbiol. 189:49-58(2008).
CC   -!- FUNCTION: May play a role in resistance to osmotic downshock.
CC       {ECO:0000269|PubMed:17665170}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- INDUCTION: Induced by high salt concentrations.
CC       {ECO:0000269|PubMed:17665170}.
CC   -!- DISRUPTION PHENOTYPE: Cells lacking this gene grow normally in minimal
CC       medium or in high-osmolarity environments, and exhibit the same
CC       survival capacity as the wild-type strain upon a drastic osmotic
CC       downshift. They sporulate and germinate normally.
CC       {ECO:0000269|PubMed:17665170, ECO:0000269|PubMed:18310427}.
CC   -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR   EMBL; D83967; BAA23393.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12623.1; -; Genomic_DNA.
DR   PIR; G69807; G69807.
DR   RefSeq; NP_388675.1; NC_000964.3.
DR   RefSeq; WP_010886444.1; NZ_JNCM01000032.1.
DR   AlphaFoldDB; O35043; -.
DR   SMR; O35043; -.
DR   STRING; 224308.BSU07940; -.
DR   PaxDb; O35043; -.
DR   PRIDE; O35043; -.
DR   EnsemblBacteria; CAB12623; CAB12623; BSU_07940.
DR   GeneID; 936137; -.
DR   KEGG; bsu:BSU07940; -.
DR   PATRIC; fig|224308.43.peg.834; -.
DR   eggNOG; COG0668; Bacteria.
DR   InParanoid; O35043; -.
DR   OMA; FLIYERQ; -.
DR   PhylomeDB; O35043; -.
DR   BioCyc; BSUB:BSU07940-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008381; F:mechanosensitive ion channel activity; IBA:GO_Central.
DR   GO; GO:0071470; P:cellular response to osmotic stress; IBA:GO_Central.
DR   Gene3D; 2.30.30.60; -; 1.
DR   InterPro; IPR010920; LSM_dom_sf.
DR   InterPro; IPR006685; MscS_channel.
DR   InterPro; IPR011014; MscS_channel_TM-2.
DR   InterPro; IPR023408; MscS_dom_sf.
DR   InterPro; IPR045276; YbiO_bact.
DR   PANTHER; PTHR30460; PTHR30460; 1.
DR   Pfam; PF00924; MS_channel; 1.
DR   SUPFAM; SSF50182; SSF50182; 1.
DR   SUPFAM; SSF82861; SSF82861; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..280
FT                   /note="Uncharacterized MscS family protein YfkC"
FT                   /id="PRO_0000367892"
FT   TRANSMEM        15..35
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        68..88
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   280 AA;  32206 MW;  7FF0F1EEA826FE0F CRC64;
     MRMKETLTEI FQNKIVDILL VAVILWIGVF IINRLVQLFF KRTDFIEEKK EKTIESLVRS
     VTQYTATIGF IFYVISLFVH DFGKILAGAG VAGIVIGFGA QSLIKDVLAG VFLIYERQLH
     KGDYVTVNNL FNGTVEEIGL RSLQIREWSG KLLTISNGEV RQIENYNIDF MRITESFLIS
     FKEDPDRVYS VLEEACDMLN EELRDSLKRD EFGNPTEPFQ IHGITALNKI NRGVEFTVKG
     MVKDDDYFSA SLAVRRVLVR QLYQNNVQML EEAVRIERTQ