YFKO_BACSU
ID YFKO_BACSU Reviewed; 221 AA.
AC O34475;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Putative NAD(P)H nitroreductase YfkO;
DE EC=1.-.-.-;
GN Name=yfkO; OrderedLocusNames=BSU07830;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / AC327;
RA Sekiguchi J., Yamamoto H., Uchiyama S., Fajar A.;
RT "Nucleotide sequence analysis of B. subtilis chromosome in 74 degree
RT region.";
RL Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP PROTEIN SEQUENCE OF 2-21, SUBUNIT, AND COFACTOR.
RX PubMed=15252706; DOI=10.1007/s00203-004-0701-5;
RA Seo D., Kamino K., Inoue K., Sakurai H.;
RT "Purification and characterization of ferredoxin-NADP+ reductase encoded by
RT Bacillus subtilis yumC.";
RL Arch. Microbiol. 182:80-89(2004).
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000269|PubMed:15252706};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:15252706}.
CC -!- SIMILARITY: Belongs to the nitroreductase family. {ECO:0000305}.
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DR EMBL; D83967; BAA23405.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12612.1; -; Genomic_DNA.
DR PIR; B69809; B69809.
DR RefSeq; NP_388664.1; NC_000964.3.
DR RefSeq; WP_003243096.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; O34475; -.
DR SMR; O34475; -.
DR STRING; 224308.BSU07830; -.
DR PaxDb; O34475; -.
DR EnsemblBacteria; CAB12612; CAB12612; BSU_07830.
DR GeneID; 939189; -.
DR KEGG; bsu:BSU07830; -.
DR PATRIC; fig|224308.179.peg.847; -.
DR eggNOG; COG0778; Bacteria.
DR InParanoid; O34475; -.
DR OMA; PWHFVVA; -.
DR PhylomeDB; O34475; -.
DR BioCyc; BSUB:BSU07830-MON; -.
DR SABIO-RK; O34475; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0018545; F:NAD(P)H nitroreductase activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0046256; P:2,4,6-trinitrotoluene catabolic process; IBA:GO_Central.
DR CDD; cd02149; NfsB-like; 1.
DR Gene3D; 3.40.109.10; -; 1.
DR InterPro; IPR033878; NfsB-like.
DR InterPro; IPR029479; Nitroreductase.
DR InterPro; IPR000415; Nitroreductase-like.
DR Pfam; PF00881; Nitroreductase; 1.
DR SUPFAM; SSF55469; SSF55469; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Flavoprotein; FMN; NAD; NADP; Oxidoreductase;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:15252706"
FT CHAIN 2..221
FT /note="Putative NAD(P)H nitroreductase YfkO"
FT /id="PRO_0000072719"
FT BINDING 15..17
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 73..75
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 157..162
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 169..170
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
FT BINDING 211
FT /ligand="FMN"
FT /ligand_id="ChEBI:CHEBI:58210"
FT /evidence="ECO:0000250"
SQ SEQUENCE 221 AA; 25628 MW; 3D911EAC3402F599 CRC64;
MADLKTQILD AYNFRHATKE FDPNKKVSDS DFEFILETGR LSPSSLGLEP WKFVVVQNPE
FREKLREYTW GAQKQLPTAS HFVLILARTA KDIKYNADYI KRHLKEVKQM PQDVYEGYLS
KTEEFQKNDL HLLESDRTLF DWASKQTYIA LGNMMTAAAQ IGVDSCPIEG FQYDHIHRIL
EEEGLLENGS FDISVMVAFG YRVRDPRPKT RSAVEDVVKW V
