YFLS_BACSU
ID YFLS_BACSU Reviewed; 478 AA.
AC O34726; Q79ET4;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Putative malate transporter YflS;
GN Name=yflS; OrderedLocusNames=BSU07570;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / AC327;
RX PubMed=9272861; DOI=10.1016/s0378-1119(97)00130-3;
RA Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J.;
RT "Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of
RT the Bacillus subtilis genome reveal genes for a new two-component system,
RT three spore germination proteins, an iron uptake system and a general
RT stress response protein.";
RL Gene 194:191-199(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP REGULATION BY MALK/MALR.
RX PubMed=12949159; DOI=10.1099/mic.0.26257-0;
RA Tanaka K., Kobayashi K., Ogasawara N.;
RT "The Bacillus subtilis YufLM two-component system regulates the expression
RT of the malate transporters MaeN (YufR) and YflS, and is essential for
RT utilization of malate in minimal medium.";
RL Microbiology 149:2317-2329(2003).
CC -!- FUNCTION: Might be a malate transporter.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Activated by the two-component regulatory system MalK/MalR
CC in response to malate. The regulator MalR binds to the promoter region
CC of yflS.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC DIT1 subfamily. {ECO:0000305}.
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DR EMBL; D86417; BAA22312.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12586.1; -; Genomic_DNA.
DR PIR; F69811; F69811.
DR RefSeq; NP_388638.1; NC_000964.3.
DR RefSeq; WP_003233725.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; O34726; -.
DR SMR; O34726; -.
DR STRING; 224308.BSU07570; -.
DR PaxDb; O34726; -.
DR EnsemblBacteria; CAB12586; CAB12586; BSU_07570.
DR GeneID; 936122; -.
DR KEGG; bsu:BSU07570; -.
DR PATRIC; fig|224308.179.peg.822; -.
DR eggNOG; COG0471; Bacteria.
DR InParanoid; O34726; -.
DR OMA; GLNPNAW; -.
DR PhylomeDB; O34726; -.
DR BioCyc; BSUB:BSU07570-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR030676; CitT-rel.
DR InterPro; IPR001898; SLC13A/DASS.
DR PANTHER; PTHR42826; PTHR42826; 1.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR PIRSF; PIRSF002457; DASS; 1.
DR TIGRFAMs; TIGR00785; dass; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..478
FT /note="Putative malate transporter YflS"
FT /id="PRO_0000172502"
FT TRANSMEM 12..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 64..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..244
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..319
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 398..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 450..472
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 478 AA; 51432 MW; FB49B0F20D9FEFB6 CRC64;
MASEKDAGKQ SAVKLVPLLI TVAVGLIIWF IPAPSGLEPK AWHLFAIFVA TIIGFISKPL
PMGAIAIFAL AVTALTGTLS IEDTLSGFGN KTIWLIVIAF FISRGFIKTG LGARISYVFV
QKFGKKTLGL SYSLLFSDLI LSPAIPSNTA RAGGIIFPII RSLSETFGSS PANGTERKIG
AFLLKTGFQG NLITSAMFLT AMAANPLIAK LAHDVAGVDL TWTSWAIAAI VPGLVSLIIT
PLVIYKLYPP EIKETPDAAK IATEKLKEMG PFKKSELSMV IVFLLVLVLW IFGGSFNIDA
TTTALIGLAV LLLSQVLTWD DIKKEQGAWD TLTWFAALVM LANFLNELGM VSWFSNAMKS
SVSGFSWIVA FIILIVVYYY SHYFFASATA HISAMYSAFL AVVVAAGAPP LLAALSLAFI
SNLFGSTTHY GSGAAPVFFG AGYIPQGKWW SIGFILSIVH IIVWLVIGGL WWKVLGIW
