YFMC_BACSU
ID YFMC_BACSU Reviewed; 315 AA.
AC O34348; Q79ET3;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Fe(3+)-citrate-binding protein YfmC;
DE AltName: Full=Ferric-citrate-binding protein;
DE Flags: Precursor;
GN Name=yfmC; OrderedLocusNames=BSU07520;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / AC327;
RX PubMed=9272861; DOI=10.1016/s0378-1119(97)00130-3;
RA Yamamoto H., Uchiyama S., Nugroho F.A., Sekiguchi J.;
RT "Cloning and sequencing of a 35.7 kb in the 70 degree-73 degree region of
RT the Bacillus subtilis genome reveal genes for a new two-component system,
RT three spore germination proteins, an iron uptake system and a general
RT stress response protein.";
RL Gene 194:191-199(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SUBCELLULAR LOCATION.
RC STRAIN=168;
RX PubMed=14730579; DOI=10.1002/elps.200305676;
RA Bunai K., Ariga M., Inoue T., Nozaki M., Ogane S., Kakeshita H., Nemoto T.,
RA Nakanishi H., Yamane K.;
RT "Profiling and comprehensive expression analysis of ABC transporter solute-
RT binding proteins of Bacillus subtilis membrane based on a proteomic
RT approach.";
RL Electrophoresis 25:141-155(2004).
RN [4]
RP FUNCTION, AND INDUCTION.
RX PubMed=16672620; DOI=10.1128/jb.188.10.3664-3673.2006;
RA Ollinger J., Song K.-B., Antelmann H., Hecker M., Helmann J.D.;
RT "Role of the Fur regulon in iron transport in Bacillus subtilis.";
RL J. Bacteriol. 188:3664-3673(2006).
CC -!- FUNCTION: Part of the ABC transporter complex YfmCDEF involved in
CC citrate-dependent Fe(3+) import. Binds citrate-dependent Fe(3+) and
CC delivers it to the surface of YfmDE (Probable).
CC {ECO:0000305|PubMed:16672620}.
CC -!- SUBUNIT: The complex is composed of one ATP-binding protein (YfmF), two
CC transmembrane proteins (YfmD and YfmE) and a solute-binding protein
CC (YfmC). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:14730579};
CC Lipid-anchor {ECO:0000305|PubMed:14730579}.
CC -!- INDUCTION: Transcriptionally regulated by fur.
CC {ECO:0000269|PubMed:16672620}.
CC -!- SIMILARITY: Belongs to the bacterial solute-binding protein 8 family.
CC {ECO:0000305}.
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DR EMBL; D86417; BAA22317.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12581.1; -; Genomic_DNA.
DR PIR; B69812; B69812.
DR RefSeq; NP_388633.1; NC_000964.3.
DR RefSeq; WP_003243996.1; NZ_JNCM01000032.1.
DR AlphaFoldDB; O34348; -.
DR SMR; O34348; -.
DR STRING; 224308.BSU07520; -.
DR jPOST; O34348; -.
DR PaxDb; O34348; -.
DR PRIDE; O34348; -.
DR EnsemblBacteria; CAB12581; CAB12581; BSU_07520.
DR GeneID; 936114; -.
DR KEGG; bsu:BSU07520; -.
DR PATRIC; fig|224308.179.peg.818; -.
DR eggNOG; COG4594; Bacteria.
DR InParanoid; O34348; -.
DR OMA; NDEIWQT; -.
DR PhylomeDB; O34348; -.
DR BioCyc; BSUB:BSU07520-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0055072; P:iron ion homeostasis; IEA:UniProtKB-KW.
DR InterPro; IPR002491; ABC_transptr_periplasmic_BD.
DR Pfam; PF01497; Peripla_BP_2; 1.
DR PROSITE; PS50983; FE_B12_PBP; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion transport; Iron; Iron transport; Lipoprotein; Membrane;
KW Palmitate; Reference proteome; Signal; Transport.
FT SIGNAL 1..18
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 19..315
FT /note="Fe(3+)-citrate-binding protein YfmC"
FT /id="PRO_0000361675"
FT DOMAIN 60..315
FT /note="Fe/B12 periplasmic-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00344"
FT REGION 27..52
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 19
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 19
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 315 AA; 35021 MW; 2DA3BFFC49EFEC39 CRC64;
MRTYSNKLIA IMSVLLLACL IVSGCSSSQN NNGSGKSESK DSRVIHDEEG KTTVSGTPKR
VVVLELSFLD AVHNLGITPV GIADDNKKDM IKKLVGSSID YTSVGTRSEP NLEVISSLKP
DLIIADAERH KNIYKQLKKI APTIELKSRE ATYDETIDSF TTIAKALNKE DEGKEKLAEH
KKVINDLKAE LPKDENRNIV LGVARADSFQ LHTSSSYDGE IFKMLGFTHA VKSDNAYQEV
SLEQLSKIDP DILFISANEG KTIVDEWKTN PLWKNLKAVK NGQVYDADRD TWTRFRGIKS
SETSAKDVLK KVYNK
