CBF3C_YEAST
ID CBF3C_YEAST Reviewed; 478 AA.
AC P35203; D6VZR7;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Centromere DNA-binding protein complex CBF3 subunit C;
DE AltName: Full=Chromosome transmission fidelity protein 13;
DE AltName: Full=Kinetochore protein CTF13;
GN Name=CTF13; Synonyms=CBF3C; OrderedLocusNames=YMR094W;
GN ORFNames=YM6543.01, YM9582.19;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8500169; DOI=10.1016/0092-8674(93)90255-o;
RA Doheny K.F., Sorger P.K., Hyman A.A., Tugendreich S., Spencer F.,
RA Hieter P.;
RT "Identification of essential components of the S. cerevisiae kinetochore.";
RL Cell 73:761-774(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP PROTEIN SEQUENCE OF 2-8; 10-17 AND 342-349.
RX PubMed=7957085; DOI=10.1002/j.1460-2075.1994.tb06851.x;
RA Lechner J.;
RT "A zinc finger protein, essential for chromosome segregation, constitutes a
RT putative DNA binding subunit of the Saccharomyces cerevisiae kinetochore
RT complex, Cbf3.";
RL EMBO J. 13:5203-5211(1994).
RN [5]
RP ASSEMBLY OF THE CBF3 COMPLEX, AND INTERACTION WITH CBF3D AND SGT1.
RX PubMed=15090617; DOI=10.1091/mbc.e03-12-0887;
RA Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B.;
RT "Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes
RT required for proper kinetochore function.";
RL Mol. Biol. Cell 15:3366-3378(2004).
CC -!- FUNCTION: Acts as central component of the centromere DNA-binding
CC protein complex CBF3, which is essential for chromosome segregation and
CC movement of centromeres along microtubules. CBF3 is required for the
CC recruitment of other kinetochore complexes to CEN DNA. It plays a role
CC in the attachment of chromosomes to the spindle and binds selectively
CC to a highly conserved DNA sequence called CDEIII, found in centromers
CC and in several promoters. The association of CBF3C with CBF3D and SGT1
CC is required for CBF3C activation and CBF3 assembly.
CC -!- SUBUNIT: Component of the CBF3 copmplex, which is formed of CBF3A/CBF2,
CC CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF3C interacts with CBF3D and SGT1.
CC {ECO:0000269|PubMed:15090617}.
CC -!- INTERACTION:
CC P35203; P32504: CBF2; NbExp=5; IntAct=EBI-4085, EBI-4069;
CC P35203; P40969: CEP3; NbExp=2; IntAct=EBI-4085, EBI-4077;
CC P35203; P53267: DAM1; NbExp=2; IntAct=EBI-4085, EBI-23268;
CC P35203; Q08446: SGT1; NbExp=3; IntAct=EBI-4085, EBI-17070;
CC P35203; P52286: SKP1; NbExp=8; IntAct=EBI-4085, EBI-4090;
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere.
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DR EMBL; L10063; AAA34536.1; -; Genomic_DNA.
DR EMBL; Z49259; CAA89241.1; -; Genomic_DNA.
DR EMBL; Z49807; CAA89895.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09991.1; -; Genomic_DNA.
DR PIR; A40747; A40747.
DR RefSeq; NP_013812.1; NM_001182594.1.
DR PDB; 6FE8; EM; 3.70 A; D=2-478.
DR PDB; 6GSA; EM; 4.20 A; D=2-478.
DR PDB; 6GYP; EM; 3.60 A; A=1-478.
DR PDB; 6GYS; EM; 4.40 A; A/H=1-478.
DR PDB; 6GYU; EM; 3.00 A; A=1-478.
DR PDB; 7K79; EM; 4.00 A; K=2-478.
DR PDBsum; 6FE8; -.
DR PDBsum; 6GSA; -.
DR PDBsum; 6GYP; -.
DR PDBsum; 6GYS; -.
DR PDBsum; 6GYU; -.
DR PDBsum; 7K79; -.
DR AlphaFoldDB; P35203; -.
DR SMR; P35203; -.
DR BioGRID; 35269; 198.
DR ComplexPortal; CPX-1898; CBF3 complex.
DR DIP; DIP-1277N; -.
DR IntAct; P35203; 12.
DR MINT; P35203; -.
DR STRING; 4932.YMR094W; -.
DR iPTMnet; P35203; -.
DR MaxQB; P35203; -.
DR PaxDb; P35203; -.
DR PRIDE; P35203; -.
DR EnsemblFungi; YMR094W_mRNA; YMR094W; YMR094W.
DR GeneID; 855119; -.
DR KEGG; sce:YMR094W; -.
DR SGD; S000004700; CTF13.
DR VEuPathDB; FungiDB:YMR094W; -.
DR eggNOG; ENOG502QRJV; Eukaryota.
DR HOGENOM; CLU_046445_0_0_1; -.
DR InParanoid; P35203; -.
DR OMA; VYFHLDG; -.
DR BioCyc; YEAST:G3O-32794-MON; -.
DR PRO; PR:P35203; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P35203; protein.
DR GO; GO:0031518; C:CBF3 complex; IDA:SGD.
DR GO; GO:0000776; C:kinetochore; IDA:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0008301; F:DNA binding, bending; IDA:SGD.
DR GO; GO:0051382; P:kinetochore assembly; IDA:SGD.
DR GO; GO:0000921; P:septin ring assembly; IMP:SGD.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR032675; LRR_dom_sf.
PE 1: Evidence at protein level;
KW 3D-structure; Centromere; Chromosome; Direct protein sequencing;
KW DNA-binding; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:7957085"
FT CHAIN 2..478
FT /note="Centromere DNA-binding protein complex CBF3 subunit
FT C"
FT /id="PRO_0000089370"
FT REGION 206..251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..232
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 6..11
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 14..23
FT /evidence="ECO:0007829|PDB:6GYU"
FT TURN 36..40
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 43..46
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 59..65
FT /evidence="ECO:0007829|PDB:6GYU"
FT TURN 66..70
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 71..74
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 75..77
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 82..93
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 98..108
FT /evidence="ECO:0007829|PDB:6GYU"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 129..132
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 144..150
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 152..155
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 177..189
FT /evidence="ECO:0007829|PDB:6GYU"
FT TURN 193..195
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 196..198
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 258..265
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 276..278
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 284..287
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 297..300
FT /evidence="ECO:0007829|PDB:6GYU"
FT TURN 303..308
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 311..314
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 318..320
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 322..324
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 336..339
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 343..345
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 347..349
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 358..361
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 365..367
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 372..379
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 384..390
FT /evidence="ECO:0007829|PDB:6GYU"
FT TURN 400..402
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 406..408
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 411..413
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 415..418
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 420..423
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 424..434
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 448..450
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 452..455
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 456..459
FT /evidence="ECO:0007829|PDB:6GYU"
FT HELIX 460..462
FT /evidence="ECO:0007829|PDB:6GYU"
FT STRAND 475..478
FT /evidence="ECO:0007829|PDB:6GYU"
SQ SEQUENCE 478 AA; 56336 MW; 77B1C012FD74E56F CRC64;
MPSFNPVRFL ELPIDIRKEV YFHLDGNFCG AHPYPIDILY KSNDVELPGK PSYKRSKRSK
KLLRYMYPVF ATYLNIFEYS PQLIEKWLEY AFWLRYDCLV LDCFKVNHLY DGTLIDALEW
TYLDNELRLA YFNKASMLEV WYTFKEYKKW VIDSVAFDEL DLLNVSNIQF NIDNLTPQLV
DKCLSILEQK DLFATIGEVQ FGQDEEVGEE KDVDVSGANS DENSSPSSTI KNKKRSASKR
SHSDNGNVGA THNQLTSISV IRTIRSMESM KSLRKITVRG EKLYELLINF HGFRDNPGKT
ISYIVKRRIN EIRLSRMNQI SRTGLADFTR WDNLQKLVLS RVAYIDLNSI VFPKNFKSLT
MKRVSKIKWW NIEENILKEL KVDKRTFKSL YIKEDDSKFT KFFNLRHTRI KELDKSEINQ
ITYLRCQAIV WLSFRTLNHI KLQNVSEVFN NIIVPRALFD SKRVEIYRCE KISQVLVI
