ID   YGAY_ECOLI              Reviewed;         394 AA.
AC   P76628; P76629; Q2MAC3;
DT   10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2002, sequence version 2.
DT   25-MAY-2022, entry version 136.
DE   RecName: Full=Putative uncharacterized transporter YgaY;
GN   Name=ygaY; OrderedLocusNames=b2681, JW2655; ORFNames=b2680;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   CONCEPTUAL TRANSLATION.
RA   Rudd K.E.;
RL   Unpublished observations (JAN-1999).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. There seems to be a
CC       natural frameshift that produces two separate ORFs. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAE76783.1; Type=Frameshift; Evidence={ECO:0000305};
CC       Sequence=U00096; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; BAE76783.1; ALT_FRAME; Genomic_DNA.
DR   PIR; A65048; A65048.
DR   PIR; B65048; B65048.
DR   RefSeq; WP_000165699.1; NZ_STEB01000042.1.
DR   AlphaFoldDB; P76628; -.
DR   SMR; P76628; -.
DR   BioGRID; 4262259; 8.
DR   STRING; 316407.85675521; -.
DR   TCDB; 2.A.1.36.3; the major facilitator superfamily (mfs).
DR   PRIDE; P76628; -.
DR   EnsemblBacteria; BAE76783; BAE76783; BAE76783.
DR   GeneID; 66673450; -.
DR   KEGG; ecj:JW2655; -.
DR   eggNOG; COG2814; Bacteria.
DR   HOGENOM; CLU_180524_0_0_6; -.
DR   InParanoid; P76628; -.
DR   PhylomeDB; P76628; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 2.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   5: Uncertain;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..394
FT                   /note="Putative uncharacterized transporter YgaY"
FT                   /id="PRO_0000173407"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        50..70
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        79..99
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        100..120
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        166..186
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        218..238
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        291..311
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        337..357
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   394 AA;  41655 MW;  23EDB3DCFA79BE76 CRC64;
     MTKPNHELSP ALIVLMSIAT GLAVASNYYA QPLLDTIARN FSLSASSAGF IVTAAQLGYA
     AGLLFLVPLG DMFERRRLIV SMTLLAAGGM LITASSQSLA MMILGTALTG LFSVVAQILV
     PLAATLASPD KRGKVVGTIM SGLLLGILLA RTVAGLLANL GGWRTVFWVA SVLMALMALA
     LWRGLPQMKS ETHLNYPQLL GSVFSMFISD KILRTRALLG CLTFANFSIL WTSMAFLLAA
     PPFNYSDGVI GLFGLAGAAG ALGARPAGGF ADKGKSHHTT TFGLLLLLLS WLAIWFGHTS
     VLALIIGILV LDLTVQGVHI TNQTVIYRIH PDARNRLTAG YMTSYFIGGA AGSLISASAW
     QHGGWAGVCL AGATIALVNL LVWWRGFHRQ EAAN