CBH1_SCHPO
ID CBH1_SCHPO Reviewed; 514 AA.
AC O14423; O14294;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 2.
DT 25-MAY-2022, entry version 149.
DE RecName: Full=CENP-B homolog protein 1;
DE Short=CBHP-1;
GN Name=cbh1; Synonyms=cbh; ORFNames=SPAC9E9.10c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, AND FUNCTION.
RC STRAIN=972 / ATCC 24843;
RX PubMed=9237993; DOI=10.1073/pnas.94.16.8427;
RA Lee J.-K., Huberman J.A., Hurwitz J.;
RT "Purification and characterization of a CENP-B homologue protein that binds
RT to the centromeric K-type repeat DNA of Schizosaccharomyces pombe.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:8427-8432(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Binds to centromeric K-type repeat DNA and ARS3002 DNA. The
CC CBH-binding consensus sequence is Py-Pu-A-T-A-T-Py-Pu-T-A.
CC {ECO:0000269|PubMed:9237993}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Chromosome, centromere.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U86754; AAB80698.1; -; mRNA.
DR EMBL; CU329670; CAB16408.1; -; Genomic_DNA.
DR PIR; T39217; T39217.
DR RefSeq; NP_594583.1; NM_001020012.2.
DR AlphaFoldDB; O14423; -.
DR SMR; O14423; -.
DR BioGRID; 279715; 81.
DR IntAct; O14423; 3.
DR STRING; 4896.SPAC9E9.10c.1; -.
DR MaxQB; O14423; -.
DR PaxDb; O14423; -.
DR EnsemblFungi; SPAC9E9.10c.1; SPAC9E9.10c.1:pep; SPAC9E9.10c.
DR GeneID; 2543290; -.
DR KEGG; spo:SPAC9E9.10c; -.
DR PomBase; SPAC9E9.10c; cbh1.
DR VEuPathDB; FungiDB:SPAC9E9.10c; -.
DR eggNOG; KOG3105; Eukaryota.
DR HOGENOM; CLU_018294_0_3_1; -.
DR InParanoid; O14423; -.
DR OMA; PEENVIM; -.
DR PhylomeDB; O14423; -.
DR PRO; PR:O14423; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000785; C:chromatin; NAS:PomBase.
DR GO; GO:0000779; C:condensed chromosome, centromeric region; IDA:PomBase.
DR GO; GO:0072686; C:mitotic spindle; HDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0019237; F:centromeric DNA binding; IDA:PomBase.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR InterPro; IPR004875; DDE_SF_endonuclease_dom.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR041188; HTH_ABP1_N.
DR InterPro; IPR006600; HTH_CenpB_DNA-bd_dom.
DR Pfam; PF03184; DDE_1; 1.
DR Pfam; PF18107; HTH_ABP1_N; 1.
DR Pfam; PF03221; HTH_Tnp_Tc5; 1.
DR SMART; SM00674; CENPB; 1.
DR SUPFAM; SSF46689; SSF46689; 2.
DR PROSITE; PS51253; HTH_CENPB; 1.
PE 1: Evidence at protein level;
KW Centromere; Chromosome; Direct protein sequencing; DNA-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..514
FT /note="CENP-B homolog protein 1"
FT /id="PRO_0000126134"
FT DOMAIN 69..144
FT /note="HTH CENPB-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00583"
FT CONFLICT 168
FT /note="I -> M (in Ref. 1; AAB80698)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 59865 MW; A48102EBAD0BB1E3 CRC64;
MPPIRQAVSL AEKKAIRDYY NQSAHRIPQK EVTEWFRKTY NKDLSQSTIS EILSPKYSYL
DDGSVRLGDI KKIRAPKFPL LDNAVYEWLQ QREVEGLPIS GDMIKQAATR FWSKIPAYAS
LPLPDFSNGW LDKFRRRHYI QQSAVNQALE SIKFEVFREY PVHIQEFIRP YAQKDIFCVG
GTSLFWKLTP NKQNLDYQEI QGMKRGKAKV SLIMCCNADG SERLPLWIVG YAQNPRSFEQ
CGIFPKSMNF EWKWNGRASI SPIIMEEWLR WFDTRMQGRK VLLMLESNDT YQFGVESVRR
FKGGFQNTSV FRIPEKTLDI KSPFEQGIVD TFKANYRRYW LQYSLNQINI LRDPLKAVNV
LKAIRWMLWS WNFGVSEKTI QASFLRSGLL GPHSEAEGQG MESYQKAIME IGNLISSKGS
EAVKQINEYI RPSEEDETKL HQDAVDTVAA EFLEERRFES DEEEDVEPQI SNVEAAMAFE
VLIKYFEQHE NGDPSFVLDL YRRQRVIAPN NLIA
