ID   YGEY_ECOL6              Reviewed;         403 AA.
AC   P65808; Q46805;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Uncharacterized protein YgeY;
GN   Name=ygeY; OrderedLocusNames=c3450;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000250};
CC       Note=Binds 2 Zn(2+) or Co(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M20A family. {ECO:0000305}.
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DR   EMBL; AE014075; AAN81895.1; -; Genomic_DNA.
DR   RefSeq; WP_001107125.1; NC_004431.1.
DR   AlphaFoldDB; P65808; -.
DR   SMR; P65808; -.
DR   STRING; 199310.c3450; -.
DR   PRIDE; P65808; -.
DR   EnsemblBacteria; AAN81895; AAN81895; c3450.
DR   GeneID; 67415244; -.
DR   KEGG; ecc:c3450; -.
DR   eggNOG; COG0624; Bacteria.
DR   HOGENOM; CLU_021802_2_1_6; -.
DR   OMA; YGAKIMK; -.
DR   BioCyc; ECOL199310:C3450-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR001261; ArgE/DapE_CS.
DR   InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR   InterPro; IPR002933; Peptidase_M20.
DR   InterPro; IPR017706; Peptidase_M20/DapE_YgeY.
DR   InterPro; IPR011650; Peptidase_M20_dimer.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   SUPFAM; SSF55031; SSF55031; 1.
DR   TIGRFAMs; TIGR03526; selenium_YgeY; 1.
DR   PROSITE; PS00758; ARGE_DAPE_CPG2_1; 1.
PE   3: Inferred from homology;
KW   Cobalt; Hydrolase; Metal-binding; Metalloprotease; Protease; Zinc.
FT   CHAIN           1..403
FT                   /note="Uncharacterized protein YgeY"
FT                   /id="PRO_0000185340"
FT   ACT_SITE        83
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        148
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         81
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         114
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         114
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         149
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         174
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         374
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   403 AA;  44804 MW;  87937D12244038B6 CRC64;
     MAKNIPFKLI LEKAKDYQAD MTRFLRDMVA IPSESCDEKR VVHRIKEEME KVGFDKVEID
     PMGNVLGYIG HGPRLVAMDA HIDTVGIGNI KNWDFDPYEG METDELIGGR GTSDQEGGMA
     SMVYAGKIIK DLGLEDEYTL LVTGTVQEED CDGLCWQYII EQSGIRPEFV VSTEPTDCQV
     YRGQRGRMEI RIDVQGVSCH GSAPERGDNA IFKMGPILGE LQELSQRLGY DEFLGKGTLT
     VSEIFFTSPS RCAVADSCAV SIDRRLTWGE TWEGALDEIR ALPAVQKANA VVSMYNYDRP
     SWTGLVYPTE CYFPTWKVEE DHFTVKALVN AYEGLFGKAP VVDKWTFSTN GVSIMGRHGI
     PVIGFGPGKE PEAHAPNEKT WKSHLVTCAA MYAAIPLSWL ATE