YGFK_ECO57
ID YGFK_ECO57 Reviewed; 1032 AA.
AC Q8XD75;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Putative oxidoreductase YgfK {ECO:0000250|UniProtKB:Q46811};
GN Name=ygfK; OrderedLocusNames=Z4217, ECs3751;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
CC -!- FUNCTION: Could be an iron-sulfur flavoprotein with NADPH:O(2)
CC oxidoreductase activity. {ECO:0000250|UniProtKB:Q46811}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00711};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|PROSITE-ProRule:PRU00711};
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DR EMBL; AE005174; AAG58007.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB37174.1; -; Genomic_DNA.
DR PIR; C85943; C85943.
DR PIR; G91097; G91097.
DR RefSeq; NP_311778.1; NC_002695.1.
DR RefSeq; WP_000502397.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8XD75; -.
DR SMR; Q8XD75; -.
DR STRING; 155864.EDL933_4079; -.
DR EnsemblBacteria; AAG58007; AAG58007; Z4217.
DR EnsemblBacteria; BAB37174; BAB37174; ECs_3751.
DR GeneID; 916425; -.
DR KEGG; ece:Z4217; -.
DR KEGG; ecs:ECs_3751; -.
DR PATRIC; fig|386585.9.peg.3913; -.
DR eggNOG; COG0493; Bacteria.
DR eggNOG; COG1145; Bacteria.
DR HOGENOM; CLU_014791_0_0_6; -.
DR OMA; NECGNCE; -.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR Gene3D; 1.10.1060.10; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR028261; DPD_II.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR009051; Helical_ferredxn.
DR InterPro; IPR017701; Se_rdtase_YgfK.
DR Pfam; PF14691; Fer4_20; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR TIGRFAMs; TIGR03315; Se_ygfK; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT CHAIN 1..1032
FT /note="Putative oxidoreductase YgfK"
FT /id="PRO_0000201330"
FT DOMAIN 928..958
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 938
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 941
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 944
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 948
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
SQ SEQUENCE 1032 AA; 115563 MW; C032E7D2956E738C CRC64;
MGDIMRPIPF EELLTRIFDE YQQQRSIFGI PEQQFYSPVK GKTVSVFGET CATPVGPAAG
PHTQLAQNIV TSWLTGGRFI ELKTVQILDR LELEKPCIDA EDECFNTEWS TEFTLLKAWD
EYLKAWFALH LLEAMFQPSD SGKSFIFNMS VGYNLEGIKQ PPMQQFIDNM MDASDHPKFA
QYRDTLNKLL QDDAFLARHG LQEKRESLQA LPARIPTSMV HGVTLSTMHG CPPHEIEAIC
RYMLEEKGLN TFVKLNPTLL GYARVREILD VCGFGYIGLK EESFDHDLKL TQALEMLERL
MALAKEKSLG FGVKLTNTLG TINNKGALPG EEMYMSGRAL FPLSINVAAV LSRAFDGKLP
ISYSGGASQL TIRDIFDTGI RPITMATDLL KPGGYLRLSA CMRELEGSDA WGLDHVDVER
LNRLAADALT MEYTQKHWKP EERIEVAEDL PLTDCYVAPC VTACAIKQDI PEYIRLLGEH
RYADALELIY QRNALPAITG HICDHQCQYN CTRLDYDSAL NIRELKKVAL EKGWDEYKQR
WHKPAGSGSR HPVAVIGAGP AGLAAGYFLA RAGHPVTLFE REANAGGVVK NIIPQFRIPA
ELIQHDIDFV AAHGVKFEYG CSPDLTVEQL KNQDFHYVLI ATGTDKNSGV KLAGDNQNVL
KSLPFLREYN KGTALKLGKH VVVVGAGNTA MDCARAALRV PGVEKATVVY RRSLQEMPAW
REEYEEALHD GVEFRFLNNP ERFDADGTLT LRVMSLGEPD EKGRRRPVET NETVTLHVDS
LITAIGEQQD TEALNAMGVP LDKNGWPDVD HNGETRLTDV FMIGDVQRGP SSIVAAVGTA
RRATDAILSR ENIRSHQNDK YWNNVNPAEI YQRKGDISIT LVNSDDRDAF VAQEAARCLE
CNYVCSKCVD VCPNRANVSI AVPGFQNRFQ TLHLDAYCNE CGNCAQFCPW NGKPYKDKIT
VFSLAQDFDN SSNPGFLVED CRVRVRLNNQ SWVLNIDSEG QFNNVPPELN DMCRIISHVH
QHHHYLLGRV EV
