YGFK_ECOLI
ID YGFK_ECOLI Reviewed; 1032 AA.
AC Q46811; Q2M9W2;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Putative oxidoreductase YgfK {ECO:0000305};
DE AltName: Full=Putative oxidoreductase Fe-S subunit {ECO:0000303|PubMed:22094925};
GN Name=ygfK; OrderedLocusNames=b2878, JW5923;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=K12;
RX PubMed=12480890; DOI=10.1099/00221287-148-12-3865;
RA Bebien M., Kirsch J., Mejean V., Vermeglio A.;
RT "Involvement of a putative molybdenum enzyme in the reduction of selenate
RT by Escherichia coli.";
RL Microbiology 148:3865-3872(2002).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, IRON-BINDING, AND PRELIMINARY
RP FUNCTION.
RX PubMed=22094925; DOI=10.1039/c1mt00154j;
RA Sevcenco A.M., Pinkse M.W., Wolterbeek H.T., Verhaert P.D., Hagen W.R.,
RA Hagedoorn P.L.;
RT "Exploring the microbial metalloproteome using MIRAGE.";
RL Metallomics 3:1324-1330(2011).
CC -!- FUNCTION: Could be an iron-sulfur flavoprotein with NADPH:O(2)
CC oxidoreductase activity. {ECO:0000305|PubMed:22094925}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU00711};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000255|PROSITE-ProRule:PRU00711};
CC -!- DISRUPTION PHENOTYPE: Insertion mutant is unable to reduce selenate to
CC selenium. {ECO:0000269|PubMed:12480890}.
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DR EMBL; U28375; AAA83059.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75916.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76944.1; -; Genomic_DNA.
DR PIR; F65071; F65071.
DR RefSeq; NP_417354.1; NC_000913.3.
DR RefSeq; WP_000502395.1; NZ_LN832404.1.
DR AlphaFoldDB; Q46811; -.
DR SMR; Q46811; -.
DR BioGRID; 4262325; 21.
DR BioGRID; 853310; 1.
DR DIP; DIP-28091N; -.
DR IntAct; Q46811; 2.
DR STRING; 511145.b2878; -.
DR PaxDb; Q46811; -.
DR PRIDE; Q46811; -.
DR EnsemblBacteria; AAC75916; AAC75916; b2878.
DR EnsemblBacteria; BAE76944; BAE76944; BAE76944.
DR GeneID; 949068; -.
DR KEGG; ecj:JW5923; -.
DR KEGG; eco:b2878; -.
DR PATRIC; fig|1411691.4.peg.3856; -.
DR EchoBASE; EB2873; -.
DR eggNOG; COG0493; Bacteria.
DR eggNOG; COG1145; Bacteria.
DR HOGENOM; CLU_014791_0_0_6; -.
DR OMA; NECGNCE; -.
DR BioCyc; EcoCyc:G7497-MON; -.
DR PRO; PR:Q46811; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0015930; F:glutamate synthase activity; IBA:GO_Central.
DR GO; GO:0005506; F:iron ion binding; IDA:EcoCyc.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0019676; P:ammonia assimilation cycle; IBA:GO_Central.
DR GO; GO:0006537; P:glutamate biosynthetic process; IBA:GO_Central.
DR Gene3D; 1.10.1060.10; -; 1.
DR Gene3D; 3.50.50.60; -; 2.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR028261; DPD_II.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR023753; FAD/NAD-binding_dom.
DR InterPro; IPR009051; Helical_ferredxn.
DR InterPro; IPR017701; Se_rdtase_YgfK.
DR Pfam; PF14691; Fer4_20; 1.
DR Pfam; PF07992; Pyr_redox_2; 1.
DR TIGRFAMs; TIGR03315; Se_ygfK; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 1.
PE 1: Evidence at protein level;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome.
FT CHAIN 1..1032
FT /note="Putative oxidoreductase YgfK"
FT /id="PRO_0000201329"
FT DOMAIN 928..958
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 938
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 941
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 944
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
FT BINDING 948
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00711"
SQ SEQUENCE 1032 AA; 115582 MW; 588056B51BE89648 CRC64;
MGDIMRPIPF EELLTRIFDE YQQQRSIFGI PEQQFYSPVK GKTVSVFGET CATPVGPAAG
PHTQLAQNIV TSWLTGGRFI ELKTVQILDR LELEKPCIDA EDECFNTEWS TEFTLLKAWD
EYLKAWFALH LLEAMFQPSD SGKSFIFNMS VGYNLEGIKQ PPMQQFIDNM MDASDHPKFA
QYRDTLNKLL QDDAFLARHG LQEKRESLQA LPARIPTSMV HGVTLSTMHG CPPHEIEAIC
RYMLEEKGLN TFVKLNPTLL GYARVREILD VCGFGYIGLK EESFDHDLKL TQALEMLERL
MALAKEKSLG FGVKLTNTLG TINNKGALPG EEMYMSGRAL FPLSINVAAV LSRAFDGKLP
ISYSGGASQL TIRDIFDTGI RPITMATDLL KPGGYLRLSA CMRELEGSDA WGLDHVDVER
LNRLAADALT MEYTQKHWKP EERIEVAEDL PLTDCYVAPC VTACAIKQDI PEYIRLLGEH
RYADALELIY QRNALPAITG HICDHQCQYN CTRLDYDSAL NIRELKKVAL EKGWDEYKQR
WHKPAGSGSR HPVAVIGAGP AGLAAGYFLA RAGHPVTLFE REANAGGVVK NIIPQFRIPA
ELIQHDIDFV AAHGVKFEYG CSPDLTIEQL KNQGFHYVLI ATGTDKNSGV KLAGDNQNVW
KSLPFLREYN KGTALKLGKH VVVVGAGNTA MDCARAALRV PGVEKATIVY RRSLQEMPAW
REEYEEALHD GVEFRFLNNP ERFDADGTLT LRVMSLGEPD EKGRRRPVET NETVTLLVDS
LITAIGEQQD TEALNAMGVP LDKNGWPDVD HNGETRLTDV FMIGDVQRGP SSIVAAVGTA
RRATDAILSR ENIRSHQNDK YWNNVNPAEI YQRKGDISIT LVNSDDRDAF VAQEAARCLE
CNYVCSKCVD VCPNRANVSI AVPGFQNRFQ TLHLDAYCNE CGNCAQFCPW NGKPYKDKIT
VFSLAQDFDN SSNPGFLVED CRVRVRLNNQ SWVLNIDSKG QFNNVPPELN DMCRIISHVH
QHHHYLLGRV EV
