YGFX_ECOLI
ID YGFX_ECOLI Reviewed; 135 AA.
AC Q46824; Q2M9U5;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Inner membrane protein YgfX;
DE AltName: Full=Toxin CptA {ECO:0000303|PubMed:22239607};
GN Name=ygfX {ECO:0000303|PubMed:22474332};
GN Synonyms=cptA {ECO:0000303|PubMed:22239607};
GN OrderedLocusNames=b2896, JW2864;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP PRELIMINARY (INCORRECT) FUNCTION AS A TOXIN, SUBCELLULAR LOCATION, AND
RP INTERACTION WITH FTSZ AND MREB.
RC STRAIN=B / BL21-DE3, and K12 / BW25113;
RX PubMed=22239607; DOI=10.1111/j.1574-6968.2012.02496.x;
RA Masuda H., Tan Q., Awano N., Yamaguchi Y., Inouye M.;
RT "A novel membrane-bound toxin for cell division, CptA (YgfX), inhibits
RT polymerization of cytoskeleton proteins, FtsZ and MreB, in Escherichia
RT coli.";
RL FEMS Microbiol. Lett. 328:174-181(2012).
RN [4]
RP FUNCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=22474332; DOI=10.1074/jbc.m111.293803;
RA McNeil M.B., Clulow J.S., Wilf N.M., Salmond G.P., Fineran P.C.;
RT "SdhE is a conserved protein required for flavinylation of succinate
RT dehydrogenase in bacteria.";
RL J. Biol. Chem. 287:18418-18428(2012).
RN [5]
RP NOT A TOXIN-ANTITOXIN SYSTEM, AND FUNCTION.
RC STRAIN=K12 / BW25113;
RX PubMed=23657679; DOI=10.1099/mic.0.068510-0;
RA McNeil M.B., Iglesias-Cans M.C., Clulow J.S., Fineran P.C.;
RT "YgfX (CptA) is a multimeric membrane protein that interacts with the
RT succinate dehydrogenase assembly factor SdhE (YgfY).";
RL Microbiology 159:1352-1365(2013).
CC -!- FUNCTION: A probable inner membrane protein. Has been shown not to be a
CC toxin, no effects on growth are seen in LB or minimal medium up to 6 or
CC 21 hours (respectively) after induction of expression
CC (PubMed:23657679). Interacts with cytoskeletal proteins FtsZ and MreB;
CC inhibits FtsZ GTP-dependent polymerization as well as MreB ATP-
CC dependent polymerization (PubMed:22239607). Restores production of
CC prodigiosin antibiotic (Pig) in Serratia strains with deletions of
CC sdhE-ygfX; overexpression of this protein and CptB also restores Pig
CC production to a slightly lesser extent in Serratia (PubMed:22474332).
CC {ECO:0000269|PubMed:22239607, ECO:0000269|PubMed:22474332,
CC ECO:0000269|PubMed:23657679}.
CC -!- SUBUNIT: Interacts with MreB and FtsZ; interaction with the latter
CC requires FtsZ residues 33-49 (PubMed:22239607).
CC {ECO:0000269|PubMed:22239607}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000269|PubMed:22239607}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:22239607}.
CC -!- CAUTION: Was originally thought to be the toxic component of a type II
CC toxin-antitoxin (TA) system; overexpression was shown to lead to growth
CC arrest after 5 hours, with initial elongation of cells, followed by
CC swelling (PubMed:22239607). The toxic effects were abrogated by
CC coexpression with antitoxin CptB (now sdhE) (PubMed:22239607). However
CC subsequent studies have been unable to show the toxic effect upon
CC overexpression, nor is the Serratia ortholog a toxin (PubMed:23657679).
CC {ECO:0000269|PubMed:22239607, ECO:0000269|PubMed:23657679}.
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DR EMBL; U28375; AAA83077.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75934.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76961.1; -; Genomic_DNA.
DR PIR; H65073; H65073.
DR RefSeq; NP_417372.1; NC_000913.3.
DR RefSeq; WP_000244777.1; NZ_STEB01000001.1.
DR AlphaFoldDB; Q46824; -.
DR BioGRID; 4262341; 9.
DR IntAct; Q46824; 1.
DR STRING; 511145.b2896; -.
DR jPOST; Q46824; -.
DR PaxDb; Q46824; -.
DR PRIDE; Q46824; -.
DR EnsemblBacteria; AAC75934; AAC75934; b2896.
DR EnsemblBacteria; BAE76961; BAE76961; BAE76961.
DR GeneID; 947379; -.
DR KEGG; ecj:JW2864; -.
DR KEGG; eco:b2896; -.
DR PATRIC; fig|1411691.4.peg.3837; -.
DR EchoBASE; EB2884; -.
DR eggNOG; ENOG502ZSY3; Bacteria.
DR HOGENOM; CLU_153203_0_0_6; -.
DR OMA; WLASDSM; -.
DR PhylomeDB; Q46824; -.
DR BioCyc; EcoCyc:G7509-MON; -.
DR PRO; PR:Q46824; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoCyc.
DR GO; GO:0032091; P:negative regulation of protein binding; IDA:EcoCyc.
DR GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR InterPro; IPR009883; YgfX.
DR Pfam; PF07254; Cpta_toxin; 1.
DR PIRSF; PIRSF020653; UCP020653; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Cell shape; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..135
FT /note="Inner membrane protein YgfX"
FT /id="PRO_0000169359"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..37
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..135
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 1..96
FT /note="Not required to inhibit FtsZ or MreB polymerization"
SQ SEQUENCE 135 AA; 16064 MW; CB3D38F10AAD1D98 CRC64;
MVLWQSDLRV SWRAQWLSLL IHGLVAAVIL LMPWPLSYTP LWMVLLSLVV FDCVRSQRRI
NARQGEIRLL MDGRLRWQGQ EWSIVKAPWM IKSGMMLRLR SDGGKRQHLW LAADSMDEAE
WRDLRRILLQ QETQR
