ID   YGFX_SERS3              Reviewed;         143 AA.
AC   G4V4G3; V3V7V7;
DT   15-FEB-2017, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Inner membrane protein YgfX {ECO:0000305};
GN   Name=ygfX {ECO:0000303|PubMed:22474332};
GN   Synonyms=cptA {ECO:0000303|PubMed:23657679}; ORFNames=Ser39006_01162;
OS   Serratia sp. (strain ATCC 39006).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Serratia; unclassified Serratia.
OX   NCBI_TaxID=104623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY, SUBCELLULAR LOCATION,
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=22474332; DOI=10.1074/jbc.m111.293803;
RA   McNeil M.B., Clulow J.S., Wilf N.M., Salmond G.P., Fineran P.C.;
RT   "SdhE is a conserved protein required for flavinylation of succinate
RT   dehydrogenase in bacteria.";
RL   J. Biol. Chem. 287:18418-18428(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=24336377; DOI=10.1128/genomea.01039-13;
RA   Fineran P.C., Iglesias Cans M.C., Ramsay J.P., Wilf N.M., Cossyleon D.,
RA   McNeil M.B., Williamson N.R., Monson R.E., Becher S.A., Stanton J.A.,
RA   Brugger K., Brown S.D., Salmond G.P.;
RT   "Draft genome sequence of Serratia sp. strain ATCC 39006, a model bacterium
RT   for analysis of the biosynthesis and regulation of prodigiosin, a
RT   carbapenem, and gas vesicles.";
RL   Genome Announc. 1:E01039-E01039(2013).
RN   [3]
RP   FUNCTION, PATHWAY, SUBUNIT, SUBCELLULAR LOCATION, DOMAIN, DISRUPTION
RP   PHENOTYPE, TOPOLOGY, AND MUTAGENESIS OF 1-MET--ARG-13; 1-MET--ILE-54;
RP   TRP-34 AND ASP-117.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=23657679; DOI=10.1099/mic.0.068510-0;
RA   McNeil M.B., Iglesias-Cans M.C., Clulow J.S., Fineran P.C.;
RT   "YgfX (CptA) is a multimeric membrane protein that interacts with the
RT   succinate dehydrogenase assembly factor SdhE (YgfY).";
RL   Microbiology 159:1352-1365(2013).
RN   [4]
RP   INDUCTION.
RC   STRAIN=ATCC 39006 / SC 11482;
RX   PubMed=24374335; DOI=10.1016/j.febslet.2013.12.019;
RA   McNeil M.B., Hampton H.G., Hards K.J., Watson B.N., Cook G.M.,
RA   Fineran P.C.;
RT   "The succinate dehydrogenase assembly factor, SdhE, is required for the
RT   flavinylation and activation of fumarate reductase in bacteria.";
RL   FEBS Lett. 588:414-421(2014).
CC   -!- FUNCTION: Required for production of prodigiosin antibiotic (Pig);
CC       overproduction in ygfX, sdhE or double sdhE-ygfX deletion mutants leads
CC       to nearly 10-fold increased synthesis of Pig (PubMed:22474332,
CC       PubMed:23657679). Overexpressing cells have somewhat altered cell
CC       length depending on growth phase (PubMed:23657679). Does not function
CC       as a toxin; overexpression in the presence or absence of sdhE (the
CC       putative antitoxin) has no effect on growth rate (PubMed:23657679).
CC       {ECO:0000269|PubMed:22474332, ECO:0000269|PubMed:23657679}.
CC   -!- PATHWAY: Antibiotic biosynthesis; prodigiosin biosynthesis.
CC       {ECO:0000269|PubMed:22474332, ECO:0000269|PubMed:23657679}.
CC   -!- SUBUNIT: Interacts with itself, SdhE and as in E.coli FtsZ and MreB;
CC       other interactions are shown but require validation (PubMed:23657679).
CC       {ECO:0000269|PubMed:23657679}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305|PubMed:22474332,
CC       ECO:0000305|PubMed:23657679}; Multi-pass membrane protein
CC       {ECO:0000305|PubMed:23657679}.
CC   -!- INDUCTION: Transcribed under both aerobic (with succinate or glucose)
CC       and anaerobic (with glycerol with or without fumarate) conditions
CC       (PubMed:24374335). Part of the sdhE-ygfX operon (PubMed:22474332).
CC       {ECO:0000269|PubMed:22474332, ECO:0000269|PubMed:24374335}.
CC   -!- DOMAIN: The intact transmembrane domain is required for Pig production,
CC       its replacement with 2 TMs from an E.coli membrane protein (MalF) does
CC       not allow Pig synthesis; although the chimeric protein is found in the
CC       membrane it does not self-associate. {ECO:0000269|PubMed:23657679}.
CC   -!- DISRUPTION PHENOTYPE: No effect on aerobic growth on succinate or in
CC       LB. 90% decreased synthesis of prodigiosin antibiotic, decreased
CC       transcription of flavoprotein desaturase PigA (PubMed:22474332,
CC       PubMed:23657679). {ECO:0000269|PubMed:22474332,
CC       ECO:0000269|PubMed:23657679}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ESN63754.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; HE580157; CCD22036.1; -; Genomic_DNA.
DR   EMBL; CP025084; ESN63754.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_037380577.1; NZ_CP025085.1.
DR   AlphaFoldDB; G4V4G3; -.
DR   STRING; 104623.Ser39006_01162; -.
DR   eggNOG; ENOG502ZSY3; Bacteria.
DR   OrthoDB; 1963777at2; -.
DR   UniPathway; UPA01072; -.
DR   Proteomes; UP000017700; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IMP:UniProtKB.
DR   InterPro; IPR009883; YgfX.
DR   Pfam; PF07254; Cpta_toxin; 1.
DR   PIRSF; PIRSF020653; UCP020653; 1.
PE   1: Evidence at protein level;
KW   Antibiotic biosynthesis; Cell inner membrane; Cell membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..143
FT                   /note="Inner membrane protein YgfX"
FT                   /id="PRO_0000438888"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000303|PubMed:23657679"
FT   TRANSMEM        12..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000303|PubMed:23657679"
FT   TOPO_DOM        32..35
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000303|PubMed:23657679"
FT   TRANSMEM        36..54
FT                   /note="Helical"
FT                   /evidence="ECO:0000303|PubMed:23657679"
FT   TOPO_DOM        55..143
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000303|PubMed:23657679"
FT   MUTAGEN         1..54
FT                   /note="Missing: Protein only partially membrane-associated,
FT                   no Pig production, 100% SDH activity observed."
FT                   /evidence="ECO:0000269|PubMed:23657679"
FT   MUTAGEN         1..13
FT                   /note="Missing: Less Pig production than wild-type, protein
FT                   not detectable when His-tagged."
FT                   /evidence="ECO:0000269|PubMed:23657679"
FT   MUTAGEN         34
FT                   /note="W->A: Very low Pig production, poor self-
FT                   interaction, no longer interacts with SdhE, protein
FT                   accumulates stably in membrane."
FT                   /evidence="ECO:0000269|PubMed:23657679"
FT   MUTAGEN         117
FT                   /note="D->A: Very low Pig production, no self-interaction,
FT                   no longer interacts with SdhE, protein accumulates stably
FT                   in membrane."
FT                   /evidence="ECO:0000269|PubMed:23657679"
SQ   SEQUENCE   143 AA;  17276 MW;  104C73867ECC0EB9 CRC64;
     MVQWQCNLRV SWRMQLFSLL AHGLLVLLIL LAPWPDGYMS VWLGLVTLVM FGFIRSQRNI
     KSRHGEIVLY NENHLRWQHR EWQITKRPWV LKNGILLSLR TTEGKGPRRR SLWLASDSMR
     NEEWRHLCHL LLQHKNWQSD RLM