ID   YGIQ_ECOL6              Reviewed;         739 AA.
AC   Q8FDK9;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=UPF0313 protein YgiQ {ECO:0000255|HAMAP-Rule:MF_01251};
GN   Name=ygiQ {ECO:0000255|HAMAP-Rule:MF_01251}; OrderedLocusNames=c3749;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE014075; AAN82193.1; -; Genomic_DNA.
DR   RefSeq; WP_000095222.1; NC_004431.1.
DR   AlphaFoldDB; Q8FDK9; -.
DR   STRING; 199310.c3749; -.
DR   EnsemblBacteria; AAN82193; AAN82193; c3749.
DR   KEGG; ecc:c3749; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_018288_2_0_6; -.
DR   OMA; TCMYYTG; -.
DR   BioCyc; ECOL199310:C3749-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; S-adenosyl-L-methionine.
FT   CHAIN           1..739
FT                   /note="UPF0313 protein YgiQ"
FT                   /id="PRO_0000076384"
FT   DOMAIN          372..650
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          685..739
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        696..727
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         386
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         390
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         393
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   739 AA;  83521 MW;  FB30EFDCADA53C97 CRC64;
     MSSISLIQPD RDLFSWPQYW AACFGPAPFL PMSREEMDQL GWDSCDIILV TGDAYVDHPS
     FGMAICGRML KAQGFRVGII AQPDWSSKDD FMRLGKPNLF FGVTAGNMDS MINRYTADRR
     LRHDDAYTPN NVAGKRPDRA TLVYTQRCKE AWKDVPVILG GIEASLRRTA HYDYWSDTVR
     RSVLVDSKAD MLMFGNGERP LVEVAHRLAM GEPISEIRDV RNTAIIVKEA LPGWSGVDST
     RLDTPGKIDP IPHPYGEDLP CADNKPVAPK KQEAKAVTVQ PPRPKPWEKT YVLLPSFEKV
     KGDKVLYAHA SRILHHETNP GCARALMQKH GDRYVWINPP AIPLSTEEMD SVFALPYKRV
     PHPAYGNARI PAYEMIRFSV NIMRGCFGGC SFCSITEHEG RIIQSRSEDS IINEIEAIRD
     TVPGFTGVIS DLGGPTANMY MLRCKSPRAE QTCRRLSCVY PDICPHMDTN HEPTINLYRR
     ARDLKGIKKI LIASGVRYDI AVEDPRYIKE LATHHVGGYL KIAPEHTEEG PLSKMMKPGM
     GSYDRFKELF DTYSKQAGKE QYLIPYFISA HPGTRDEDMV NLALWLKKHR FRLDQVQNFY
     PSPLANSTTM YYTGKNPLAK IGYKSEDVFV PKGDKQRRLH KALLRYHDPA NWPLIRQALE
     AMGKKHLIGS RRDCLVPAPT IEEMREARRQ NRNTRPALTK HTPMATQRQT PATAKKASST
     QSRLQNAGAK KRPKAAVGR