YGJK_ECOLI
ID YGJK_ECOLI Reviewed; 783 AA.
AC P42592; Q2M9C6;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Glucosidase YgjK;
DE EC=3.2.1.-;
DE Flags: Precursor;
GN Name=ygjK; OrderedLocusNames=b3080, JW3051;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 24-783 IN COMPLEXES WITH CALCIUM;
RP GLUCOSE; MANNOSE AND GALACTOSE, FUNCTION, AND DOMAIN.
RC STRAIN=K12;
RX PubMed=18586271; DOI=10.1016/j.jmb.2008.05.061;
RA Kurakata Y., Uechi A., Yoshida H., Kamitori S., Sakano Y., Nishikawa A.,
RA Tonozuka T.;
RT "Structural insights into the substrate specificity and function of
RT Escherichia coli K12 YgjK, a glucosidase belonging to the glycoside
RT hydrolase family 63.";
RL J. Mol. Biol. 381:116-128(2008).
CC -!- FUNCTION: Glucoside hydrolase that cleaves the alpha-1,3-glucosidic
CC linkage in nigerose. Has very low activity towards
CC maltooligosaccharides, soluble starch, nigerotriose, kojibiose and
CC trehalose. {ECO:0000269|PubMed:18586271}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 63 family. {ECO:0000305}.
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DR EMBL; U18997; AAA57881.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76115.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77130.1; -; Genomic_DNA.
DR PIR; E65096; E65096.
DR RefSeq; NP_417551.1; NC_000913.3.
DR RefSeq; WP_000695487.1; NZ_LN832404.1.
DR PDB; 3D3I; X-ray; 1.78 A; A/B=23-783.
DR PDB; 3W7S; X-ray; 1.90 A; A/B=24-783.
DR PDB; 3W7T; X-ray; 1.50 A; A/B=24-783.
DR PDB; 3W7U; X-ray; 1.99 A; A/B=24-783.
DR PDB; 3W7W; X-ray; 2.00 A; A/B=24-783.
DR PDB; 3W7X; X-ray; 2.70 A; A/B=24-783.
DR PDB; 5CA3; X-ray; 1.80 A; A/B=24-783.
DR PDB; 5GW7; X-ray; 2.20 A; A/B=24-783.
DR PDB; 6XUX; X-ray; 1.90 A; A=5-487.
DR PDBsum; 3D3I; -.
DR PDBsum; 3W7S; -.
DR PDBsum; 3W7T; -.
DR PDBsum; 3W7U; -.
DR PDBsum; 3W7W; -.
DR PDBsum; 3W7X; -.
DR PDBsum; 5CA3; -.
DR PDBsum; 5GW7; -.
DR PDBsum; 6XUX; -.
DR AlphaFoldDB; P42592; -.
DR SMR; P42592; -.
DR BioGRID; 4259266; 13.
DR ComplexPortal; CPX-2947; YgjK glucosidase complex.
DR IntAct; P42592; 5.
DR MINT; P42592; -.
DR STRING; 511145.b3080; -.
DR CAZy; GH63; Glycoside Hydrolase Family 63.
DR PaxDb; P42592; -.
DR PRIDE; P42592; -.
DR EnsemblBacteria; AAC76115; AAC76115; b3080.
DR EnsemblBacteria; BAE77130; BAE77130; BAE77130.
DR GeneID; 947596; -.
DR KEGG; ecj:JW3051; -.
DR KEGG; eco:b3080; -.
DR PATRIC; fig|1411691.4.peg.3650; -.
DR EchoBASE; EB2581; -.
DR eggNOG; COG1626; Bacteria.
DR eggNOG; COG3408; Bacteria.
DR HOGENOM; CLU_015270_0_1_6; -.
DR InParanoid; P42592; -.
DR OMA; WLEEMYP; -.
DR PhylomeDB; P42592; -.
DR BioCyc; EcoCyc:G7599-MON; -.
DR BioCyc; MetaCyc:G7599-MON; -.
DR EvolutionaryTrace; P42592; -.
DR PRO; PR:P42592; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1902687; C:glucosidase complex; IPI:ComplexPortal.
DR GO; GO:0004555; F:alpha,alpha-trehalase activity; IBA:GO_Central.
DR GO; GO:0015926; F:glucosidase activity; IDA:EcoCyc.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:1901575; P:organic substance catabolic process; IDA:ComplexPortal.
DR GO; GO:0005993; P:trehalose catabolic process; IBA:GO_Central.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001661; Glyco_hydro_37.
DR PANTHER; PTHR23403; PTHR23403; 1.
DR Pfam; PF01204; Trehalase; 1.
DR SUPFAM; SSF48208; SSF48208; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Calcium; Glycosidase; Hydrolase; Metal-binding;
KW Reference proteome; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..783
FT /note="Glucosidase YgjK"
FT /id="PRO_0000013906"
FT REGION 24..254
FT /note="N-terminal domain"
FT REGION 254..299
FT /note="Linker"
FT REGION 300..783
FT /note="A domain"
FT ACT_SITE 524
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT ACT_SITE 750
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000250"
FT BINDING 454
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 456
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 458
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 460
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 462
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT BINDING 572
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 40..44
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 48..52
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 61..64
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 70..74
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 84..101
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 109..115
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 118..124
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 126..138
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 141..152
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 154..162
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 165..169
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 172..174
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 179..182
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 184..186
FT /evidence="ECO:0007829|PDB:3D3I"
FT STRAND 189..193
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 196..200
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 204..206
FT /evidence="ECO:0007829|PDB:3W7X"
FT TURN 207..209
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 210..212
FT /evidence="ECO:0007829|PDB:3W7X"
FT STRAND 217..224
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 227..231
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 234..241
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 243..254
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 257..272
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 274..292
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 300..315
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 316..318
FT /evidence="ECO:0007829|PDB:3W7W"
FT TURN 336..338
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 345..355
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 356..358
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 360..371
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 383..385
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 391..393
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 399..401
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 418..429
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 432..452
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 457..459
FT /evidence="ECO:0007829|PDB:5GW7"
FT STRAND 464..466
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 469..471
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 478..484
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 487..492
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 494..503
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 507..510
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 512..521
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 527..529
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 534..542
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 547..550
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 554..558
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 564..572
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 574..593
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 597..617
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 621..624
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 646..648
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 652..655
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 656..659
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 665..675
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 678..681
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 683..685
FT /evidence="ECO:0007829|PDB:3W7W"
FT TURN 702..705
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 709..721
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 725..738
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 740..743
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 744..746
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 750..752
FT /evidence="ECO:0007829|PDB:3W7T"
FT TURN 754..756
FT /evidence="ECO:0007829|PDB:3W7T"
FT STRAND 759..761
FT /evidence="ECO:0007829|PDB:3W7T"
FT HELIX 766..778
FT /evidence="ECO:0007829|PDB:3W7T"
SQ SEQUENCE 783 AA; 88321 MW; AE4C37BFB78EC82B CRC64;
MKIKTILTPV TCALLISFSA HAANADNYKN VINRTGAPQY MKDYDYDDHQ RFNPFFDLGA
WHGHLLPDGP NTMGGFPGVA LLTEEYINFM ASNFDRLTVW QDGKKVDFTL EAYSIPGALV
QKLTAKDVQV EMTLRFATPR TSLLETKITS NKPLDLVWDG ELLEKLEAKE GKPLSDKTIA
GEYPDYQRKI SATRDGLKVT FGKVRATWDL LTSGESEYQV HKSLPVQTEI NGNRFTSKAH
INGSTTLYTT YSHLLTAQEV SKEQMQIRDI LARPAFYLTA SQQRWEEYLK KGLTNPDATP
EQTRVAVKAI ETLNGNWRSP GGAVKFNTVT PSVTGRWFSG NQTWPWDTWK QAFAMAHFNP
DIAKENIRAV FSWQIQPGDS VRPQDVGFVP DLIAWNLSPE RGGDGGNWNE RNTKPSLAAW
SVMEVYNVTQ DKTWVAEMYP KLVAYHDWWL RNRDHNGNGV PEYGATRDKA HNTESGEMLF
TVKKGDKEET QSGLNNYARV VEKGQYDSLE IPAQVAASWE SGRDDAAVFG FIDKEQLDKY
VANGGKRSDW TVKFAENRSQ DGTLLGYSLL QESVDQASYM YSDNHYLAEM ATILGKPEEA
KRYRQLAQQL ADYINTCMFD PTTQFYYDVR IEDKPLANGC AGKPIVERGK GPEGWSPLFN
GAATQANADA VVKVMLDPKE FNTFVPLGTA ALTNPAFGAD IYWRGRVWVD QFWFGLKGME
RYGYRDDALK LADTFFRHAK GLTADGPIQE NYNPLTGAQQ GAPNFSWSAA HLYMLYNDFF
RKQ
