YGK8_YEAST
ID YGK8_YEAST Reviewed; 140 AA.
AC P53139; D6VU38;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Uncharacterized protein YGL108C;
GN OrderedLocusNames=YGL108C; ORFNames=G3070;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9046090;
RX DOI=10.1002/(sici)1097-0061(199701)13:1<85::aid-yea53>3.0.co;2-e;
RA Paoluzi S., Minenkova O., Castagnoli L.;
RT "The genes encoding the transcription factor yTAFII60, the G4p1 protein and
RT a putative glucose transporter are contained in a 12.3 kb DNA fragment on
RT the left arm of Saccharomyces cerevisiae chromosome VII.";
RL Yeast 13:85-91(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PALMITOYLATION.
RX PubMed=16751107; DOI=10.1016/j.cell.2006.03.042;
RA Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N.,
RA Phinney B.S., Yates J.R. III, Davis N.G.;
RT "Global analysis of protein palmitoylation in yeast.";
RL Cell 125:1003-1013(2006).
RN [7]
RP MYRISTOYLATION AT GLY-2, AND PALMITOYLATION AT CYS-4 BY ERF2; PFA4 AND
RP PFA5.
RX PubMed=19416974; DOI=10.1074/jbc.m109.005447;
RA Nadolski M.J., Linder M.E.;
RT "Molecular recognition of the palmitoylation substrate Vac8 by its
RT palmitoyltransferase Pfa3.";
RL J. Biol. Chem. 284:17720-17730(2009).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:14562095};
CC Lipid-anchor {ECO:0000269|PubMed:14562095}.
CC -!- PTM: Myristoylated. {ECO:0000269|PubMed:19416974}.
CC -!- PTM: The N-myristoylated protein is further palmitoylated by ERF2, PFA4
CC and slightly by PFA5, but not by PFA3. {ECO:0000269|PubMed:16751107,
CC ECO:0000269|PubMed:19416974}.
CC -!- MISCELLANEOUS: Present with 2430 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: To S.pombe new13. {ECO:0000305}.
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DR EMBL; X97644; CAA66244.1; -; Genomic_DNA.
DR EMBL; Z72631; CAA96816.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07999.1; -; Genomic_DNA.
DR PIR; S64116; S64116.
DR RefSeq; NP_011407.3; NM_001180973.3.
DR AlphaFoldDB; P53139; -.
DR BioGRID; 33142; 34.
DR IntAct; P53139; 12.
DR MINT; P53139; -.
DR STRING; 4932.YGL108C; -.
DR iPTMnet; P53139; -.
DR SwissPalm; P53139; -.
DR MaxQB; P53139; -.
DR PaxDb; P53139; -.
DR PRIDE; P53139; -.
DR EnsemblFungi; YGL108C_mRNA; YGL108C; YGL108C.
DR GeneID; 852770; -.
DR KEGG; sce:YGL108C; -.
DR SGD; S000003076; YGL108C.
DR VEuPathDB; FungiDB:YGL108C; -.
DR eggNOG; ENOG502S7W1; Eukaryota.
DR HOGENOM; CLU_154049_0_0_1; -.
DR InParanoid; P53139; -.
DR OMA; CASKESP; -.
DR BioCyc; YEAST:G3O-30607-MON; -.
DR PRO; PR:P53139; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53139; protein.
DR GO; GO:0005829; C:cytosol; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Cell membrane; Lipoprotein; Membrane; Myristate; Palmitate;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..140
FT /note="Uncharacterized protein YGL108C"
FT /id="PRO_0000202751"
FT REGION 1..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..24
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..140
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255, ECO:0000269|PubMed:19416974"
FT LIPID 4
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000305|PubMed:19416974"
SQ SEQUENCE 140 AA; 16037 MW; 89C6AEC296CA9E57 CRC64;
MGLCGSKTQP MPSQTTTVAT KARTKPINRD TVKSKQELRH KEKKDKKKKT QLKSTTVPVV
QRKEGSKLTD TSDPSKNKVS PKEAARLAAE KRFQETNEKY NKGELGKKLA QERAKSHKTR
LMEEAEKKHA ERERENMIYD
