YHA8_YEAST
ID YHA8_YEAST Reviewed; 627 AA.
AC P38750; D3DKQ7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Uncharacterized transporter YHL008C;
GN OrderedLocusNames=YHL008C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-305; SER-546 AND THR-588, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the FNT transporter (TC 2.A.44) family.
CC {ECO:0000305}.
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DR EMBL; U11581; AAB69746.1; -; Genomic_DNA.
DR EMBL; AY558102; AAS56428.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06680.1; -; Genomic_DNA.
DR PIR; S46820; S46820.
DR RefSeq; NP_011855.1; NM_001179088.1.
DR AlphaFoldDB; P38750; -.
DR SMR; P38750; -.
DR BioGRID; 36418; 99.
DR DIP; DIP-2601N; -.
DR IntAct; P38750; 3.
DR MINT; P38750; -.
DR STRING; 4932.YHL008C; -.
DR iPTMnet; P38750; -.
DR MaxQB; P38750; -.
DR PaxDb; P38750; -.
DR PRIDE; P38750; -.
DR EnsemblFungi; YHL008C_mRNA; YHL008C; YHL008C.
DR GeneID; 856381; -.
DR KEGG; sce:YHL008C; -.
DR SGD; S000001000; YHL008C.
DR VEuPathDB; FungiDB:YHL008C; -.
DR eggNOG; ENOG502QUGF; Eukaryota.
DR HOGENOM; CLU_020549_0_0_1; -.
DR InParanoid; P38750; -.
DR OMA; KPIHVKF; -.
DR BioCyc; YEAST:G3O-31030-MON; -.
DR PRO; PR:P38750; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38750; protein.
DR GO; GO:0000324; C:fungal-type vacuole; HDA:SGD.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015513; F:high-affinity secondary active nitrite transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0140299; F:small molecule sensor activity; IMP:SGD.
DR GO; GO:0006821; P:chloride transport; IMP:SGD.
DR GO; GO:0015707; P:nitrite transport; IBA:GO_Central.
DR GO; GO:2001225; P:regulation of chloride transport; IMP:SGD.
DR Gene3D; 1.20.1080.10; -; 1.
DR InterPro; IPR023271; Aquaporin-like.
DR InterPro; IPR000292; For/NO2_transpt.
DR InterPro; IPR024002; For/NO2_transpt_CS.
DR PANTHER; PTHR30520; PTHR30520; 1.
DR Pfam; PF01226; Form_Nir_trans; 1.
DR TIGRFAMs; TIGR00790; fnt; 1.
DR PROSITE; PS01005; FORMATE_NITRITE_TP_1; 1.
DR PROSITE; PS01006; FORMATE_NITRITE_TP_2; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..627
FT /note="Uncharacterized transporter YHL008C"
FT /id="PRO_0000094729"
FT TOPO_DOM 1..32
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..66
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..113
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 114..134
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 135..165
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..192
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..218
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 240..245
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..627
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 512..537
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 605..627
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 512..529
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 305
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 546
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 588
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 627 AA; 69969 MW; 4413C989A6673FE1 CRC64;
MVDDSNYLTP HETALAVVAT AMKKARLQLD TLLINSILGG VLFSSGSFLL VAVYSEDPDI
VARNPGIVNL ITGVNFAMGL FYVVMMGADL FNSNILFFSV GVLRKAVTIY DLMISWVVSW
LGNIAGSLFV SYLFGHLSGI SSQKLWIIGS RQIIEQKVSY SFVQTFLKGI ACNFFVCLAI
YLQLMAKPIH VKFILMSFPI IDFIGIGFTH VVGDMSASFI AMLNGANVSV GKYIWKLLIP
ASLGNIVGGL FFSAVVPFYL HLVVVERDRK RLSLPEYEAR DEQPELNMDS RVVRIQKNEC
DDDATETGED LENLTEKGFA SIYNTNHDNS SYFTGRSLNS LRSIPSSVIT SDNVTMESDL
GEPVQFIPKS NSTTRSPHLG LPHNLPHNHS IKSINRHRIN KRHSLRSPPG VFPVRGMGEP
LEREKTIEDA TYDPKENELF LRRAETHNSA YVKNKKKEDD NLLRLVKTEE DREQKEYEKN
GGYNILENKP GTRLEKIITH LAENVSSREV TPPILPRTTQ DTFPHNAPAS SPAYTDDAHS
LRKANSTTLG GLFRAVSKEF HSSKDAESPD DLLKKMAAVG INRNARITAN NVAGIVNLNK
EDLDSTTRRQ KITEPKNFYN RHTSPQL
