ID   YHAJ_ECO57              Reviewed;         298 AA.
AC   P67661; P42623;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=HTH-type transcriptional regulator YhaJ;
GN   Name=yhaJ; OrderedLocusNames=Z4459, ECs3987;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
RN   [3]
RP   FUNCTION IN VIRULENCE, INDUCTION, DISRUPTION PHENOTYPE, AND DNA-BINDING.
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=26727373; DOI=10.1371/journal.ppat.1005359;
RA   Connolly J.P., Gabrielsen M., Goldstone R.J., Grinter R., Wang D.,
RA   Cogdell R.J., Walker D., Smith D.G., Roe A.J.;
RT   "A highly conserved bacterial D-serine uptake system links host metabolism
RT   and virulence.";
RL   PLoS Pathog. 12:E1005359-E1005359(2016).
CC   -!- FUNCTION: Positive regulator partially required for expression of genes
CC       in the locus of effacement (LEE) large pathogenicity island (PAI). Also
CC       partially responsible for expression of neighboring gene dlsT (yhaO)
CC       during late exponential growth. Binds to DNA of promoter 1 in LEE and
CC       DNA from the dlsT promoter region. {ECO:0000269|PubMed:26727373}.
CC   -!- INDUCTION: Induced under conditions that maximally induce expression of
CC       the locus of effacement (LEE) large pathogenicity island (PAI). Not
CC       induced by D-serine. {ECO:0000269|PubMed:26727373}.
CC   -!- DISRUPTION PHENOTYPE: Decreased expression genes in LEE and of
CC       neighboring gene dlsT. Decreased secretion of a number of substrates
CC       for type III secretion system (TTSS) encoded in the locus of effacement
CC       (LEE) including Tir, EspA and EspD; at least EspD is also decreased
CC       intracellularly. Expression of 103 genes is altered; down-regulation of
CC       LEE PAI (including the TTSS) plus other genes as well as up-regulation
CC       of 29 genes. Decreased ability to form attachment/effacing lesions on
CC       HeLa cells; those which form attachments condense host actin less well
CC       (pedestal formation) (PubMed:26727373). {ECO:0000269|PubMed:26727373}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AE005174; AAG58238.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37410.1; -; Genomic_DNA.
DR   PIR; C91127; C91127.
DR   RefSeq; NP_312014.1; NC_002695.1.
DR   RefSeq; WP_001041009.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P67661; -.
DR   SMR; P67661; -.
DR   STRING; 155864.EDL933_4328; -.
DR   EnsemblBacteria; AAG58238; AAG58238; Z4459.
DR   EnsemblBacteria; BAB37410; BAB37410; ECs_3987.
DR   GeneID; 916179; -.
DR   KEGG; ece:Z4459; -.
DR   KEGG; ecs:ECs_3987; -.
DR   PATRIC; fig|386585.9.peg.4161; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_35_1_6; -.
DR   OMA; YVHKRSG; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Transcription;
KW   Transcription regulation; Virulence.
FT   CHAIN           1..298
FT                   /note="HTH-type transcriptional regulator YhaJ"
FT                   /id="PRO_0000105797"
FT   DOMAIN          7..64
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        24..43
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   298 AA;  33256 MW;  4B6062F8ED8A3293 CRC64;
     MAKERALTLE ALRVMDAIDR RGSFAAAADE LGRVPSALSY TMQKLEEELD VVLFDRSGHR
     TKFTNVGRML LERGRVLLEA ADKLTTDAEA LARGWETHLT IVTEALVPTP AFFPLIDKLA
     AKANTQLAII TEVLAGAWER LEQGRADIVI APDMHFRSSS EINSRKLYTL MNVYVAAPDH
     PIHQEPEPLS EVTRVKYRGI AVADTARERP VLTVQLLDKQ PRLTVSTIED KRQALLAGLG
     VATMPYPMVE KDIAEGRLRV VSPESTSEID IIMAWRRDSM GEAKSWCLRE IPKLFNGK