YHAK_ECOLI
ID YHAK_ECOLI Reviewed; 233 AA.
AC P42624; Q2M9A0;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Pirin-like protein YhaK;
GN Name=yhaK; OrderedLocusNames=b3106, JW3077;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS), FUNCTION, SUBUNIT, SUBCELLULAR
RP LOCATION, AND LACK OF QUERCETINASE ACTIVITY.
RX PubMed=18561187; DOI=10.1002/prot.22128;
RA Gurmu D., Lu J., Johnson K.A., Nordlund P., Holmgren A., Erlandsen H.;
RT "The crystal structure of the protein YhaK from Escherichia coli reveals a
RT new subclass of redox sensitive enterobacterial bicupins.";
RL Proteins 74:18-31(2009).
CC -!- FUNCTION: Does not have quercetin 2,3-dioxygenase activity.
CC {ECO:0000269|PubMed:18561187}.
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:18561187}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:18561187}.
CC -!- MISCELLANEOUS: Contains Cys residues that are easily oxidized.
CC -!- SIMILARITY: Belongs to the pirin family. {ECO:0000305}.
CC -!- CAUTION: Lacks the conserved His residues required for metal binding.
CC Its function must therefore be different from the metal-dependent roles
CC proposed for other family members. {ECO:0000305}.
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DR EMBL; U18997; AAA57910.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76141.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77156.1; -; Genomic_DNA.
DR PIR; G65099; G65099.
DR RefSeq; NP_417577.1; NC_000913.3.
DR RefSeq; WP_000633576.1; NZ_LN832404.1.
DR PDB; 2VEC; X-ray; 1.85 A; A=2-233.
DR PDBsum; 2VEC; -.
DR AlphaFoldDB; P42624; -.
DR SMR; P42624; -.
DR BioGRID; 4259260; 11.
DR BioGRID; 851936; 2.
DR IntAct; P42624; 12.
DR STRING; 511145.b3106; -.
DR jPOST; P42624; -.
DR PaxDb; P42624; -.
DR PRIDE; P42624; -.
DR DNASU; 947620; -.
DR EnsemblBacteria; AAC76141; AAC76141; b3106.
DR EnsemblBacteria; BAE77156; BAE77156; BAE77156.
DR GeneID; 947620; -.
DR KEGG; ecj:JW3077; -.
DR KEGG; eco:b3106; -.
DR PATRIC; fig|1411691.4.peg.3622; -.
DR EchoBASE; EB2606; -.
DR eggNOG; COG1741; Bacteria.
DR HOGENOM; CLU_064194_1_0_6; -.
DR InParanoid; P42624; -.
DR OMA; KADYGWL; -.
DR PhylomeDB; P42624; -.
DR BioCyc; EcoCyc:G7620-MON; -.
DR EvolutionaryTrace; P42624; -.
DR PRO; PR:P42624; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR012093; Pirin.
DR InterPro; IPR003829; Pirin_N_dom.
DR InterPro; IPR041602; Quercetinase_C.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR43212; PTHR43212; 1.
DR Pfam; PF02678; Pirin; 1.
DR Pfam; PF17954; Pirin_C_2; 1.
DR PIRSF; PIRSF006232; Pirin; 1.
DR SUPFAM; SSF51182; SSF51182; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome.
FT CHAIN 1..233
FT /note="Pirin-like protein YhaK"
FT /id="PRO_0000214061"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:2VEC"
FT HELIX 17..19
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 22..25
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 40..48
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 60..77
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 89..93
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 101..105
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 108..110
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 112..120
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 129..134
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 138..147
FT /evidence="ECO:0007829|PDB:2VEC"
FT HELIX 149..151
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 156..158
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 160..166
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 171..175
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 177..188
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 190..194
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 197..202
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 207..212
FT /evidence="ECO:0007829|PDB:2VEC"
FT STRAND 214..231
FT /evidence="ECO:0007829|PDB:2VEC"
SQ SEQUENCE 233 AA; 25895 MW; 7C09B140A74E6B24 CRC64;
MITTRTARQC GQADYGWLQA RYTFSFGHYF DPKLLGYASL RVLNQEVLAP GAAFQPRTYP
KVDILNVILD GEAEYRDSEG NHVQASAGEA LLLSTQPGVS YSEHNLSKDK PLTRMQLWLD
ACPQRENPLI QKLALNMGKQ QLIASPEGAM GSLQLRQQVW LHHIVLDKGE SANFQLHGPR
AYLQSIHGKF HALTHHEEKA ALTCGDGAFI RDEANITLVA DSPLRALLID LPV
