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CBIA_METTH
ID   CBIA_METTH              Reviewed;         447 AA.
AC   O27509;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 124.
DE   RecName: Full=Cobyrinate a,c-diamide synthase {ECO:0000255|HAMAP-Rule:MF_00027};
DE            EC=6.3.5.11 {ECO:0000255|HAMAP-Rule:MF_00027};
DE   AltName: Full=Cobyrinic acid a,c-diamide synthetase {ECO:0000255|HAMAP-Rule:MF_00027};
DE   AltName: Full=Ni-sirohydrochlorin a,c-diamide synthase {ECO:0000255|HAMAP-Rule:MF_00027};
DE            EC=6.3.5.12 {ECO:0000255|HAMAP-Rule:MF_00027};
DE   AltName: Full=Ni-sirohydrochlorin a,c-diamide synthetase {ECO:0000255|HAMAP-Rule:MF_00027};
GN   Name=cbiA {ECO:0000255|HAMAP-Rule:MF_00027};
GN   Synonyms=cfbB {ECO:0000255|HAMAP-Rule:MF_00027};
GN   OrderedLocusNames=MTH_1460;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the ATP-dependent amidation of the two carboxylate
CC       groups at positions a and c of cobyrinate, using either L-glutamine or
CC       ammonia as the nitrogen source. Involved in the biosynthesis of the
CC       unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic
CC       group of methyl-coenzyme M reductase (MCR), which plays a key role in
CC       methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-
CC       dependent amidation of the two carboxylate groups at positions a and c
CC       of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen
CC       source. {ECO:0000255|HAMAP-Rule:MF_00027}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 ATP + cob(II)yrinate + 2 H2O + 2 L-glutamine = 2 ADP +
CC         cob(II)yrinate a,c diamide + 2 H(+) + 2 L-glutamate + 2 phosphate;
CC         Xref=Rhea:RHEA:26289, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:58359, ChEBI:CHEBI:58537, ChEBI:CHEBI:58894,
CC         ChEBI:CHEBI:456216; EC=6.3.5.11; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00027};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 ATP + 2 H2O + 2 L-glutamine + Ni-sirohydrochlorin = 2 ADP +
CC         2 H(+) + 2 L-glutamate + Ni-sirohydrochlorin a,c-diamide + 2
CC         phosphate; Xref=Rhea:RHEA:52896, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58359, ChEBI:CHEBI:136841,
CC         ChEBI:CHEBI:136887, ChEBI:CHEBI:456216; EC=6.3.5.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00027};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00027};
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 10/10. {ECO:0000255|HAMAP-Rule:MF_00027}.
CC   -!- DOMAIN: Comprises of two domains. The C-terminal domain contains the
CC       binding site for glutamine and catalyzes the hydrolysis of this
CC       substrate to glutamate and ammonia. The N-terminal domain is
CC       anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and
CC       catalyzes the ultimate synthesis of the diamide product. The ammonia
CC       produced via the glutaminase domain is probably translocated to the
CC       adjacent domain via a molecular tunnel, where it reacts with an
CC       activated intermediate. {ECO:0000255|HAMAP-Rule:MF_00027}.
CC   -!- MISCELLANEOUS: The a and c carboxylates of cobyrinate and Ni-
CC       sirohydrochlorin are activated for nucleophilic attack via formation of
CC       a phosphorylated intermediate by ATP. CbiA catalyzes first the
CC       amidation of the c-carboxylate, and then that of the a-carboxylate.
CC       {ECO:0000255|HAMAP-Rule:MF_00027}.
CC   -!- SIMILARITY: Belongs to the CobB/CbiA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00027}.
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DR   EMBL; AE000666; AAB85935.1; -; Genomic_DNA.
DR   PIR; E69061; E69061.
DR   RefSeq; WP_010877070.1; NC_000916.1.
DR   AlphaFoldDB; O27509; -.
DR   SMR; O27509; -.
DR   STRING; 187420.MTH_1460; -.
DR   PRIDE; O27509; -.
DR   EnsemblBacteria; AAB85935; AAB85935; MTH_1460.
DR   GeneID; 1471729; -.
DR   KEGG; mth:MTH_1460; -.
DR   PATRIC; fig|187420.15.peg.1422; -.
DR   HOGENOM; CLU_022752_2_1_2; -.
DR   OMA; MYLTNSI; -.
DR   UniPathway; UPA00148; UER00231.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0042242; F:cobyrinic acid a,c-diamide synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0015948; P:methanogenesis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.40.50.880; -; 1.
DR   HAMAP; MF_00027; CobB_CbiA; 1.
DR   InterPro; IPR004484; CbiA_synth.
DR   InterPro; IPR029062; Class_I_gatase-like.
DR   InterPro; IPR002586; CobQ/CobB/MinD/ParA_Nub-bd_dom.
DR   InterPro; IPR011698; GATase_3.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR43873; PTHR43873; 1.
DR   Pfam; PF01656; CbiA; 1.
DR   Pfam; PF07685; GATase_3; 1.
DR   SUPFAM; SSF52317; SSF52317; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00379; cobB; 1.
DR   PROSITE; PS51274; GATASE_COBBQ; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cobalamin biosynthesis; Glutamine amidotransferase; Ligase;
KW   Magnesium; Methanogenesis; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..447
FT                   /note="Cobyrinate a,c-diamide synthase"
FT                   /id="PRO_0000141277"
FT   DOMAIN          247..435
FT                   /note="GATase cobBQ-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00027"
FT   ACT_SITE        329
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00027"
FT   SITE            427
FT                   /note="Increases nucleophilicity of active site Cys"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00027"
SQ   SEQUENCE   447 AA;  48848 MW;  9A714A6E39226A26 CRC64;
     MRVVLAGTGS AVGKTTIATG IMKALSGRGV QPFKVGPDYI DPSYHTMATG NTSRNIDSFF
     MTEAQIREAF TRAMKLSGSR MGIIEGVRGL YEGISPIGDT GSTASVAKAL DAPVVLIINS
     RSLVKSAAAM VLGFRSLDRE VKIEGVILNQ VKNRRHYLKT RRAVEELTGT AVIGGIPRSS
     ELEVEQRHLG LVPAVERDTI AAQIEKWGLA MEEYIDLEAL QDIMSSAGKI RGERQPLWQR
     GNRKRVRIGV AIDEAFNFYY QENIEALEDN AASVVPFSPI HDEELPDVDA VYIGGGYPEI
     FAAELESNTS MRKSIQRFHA DGRPIFGECG GLMYLMSSID EREMCGVFPH PAEMTGRVQG
     LSYVIAEAVM DNLITEAGDK FRGHEFHYSR VLGASGGKFA FRVLRGRGIV DSLDGITSGS
     SLASYIHIHA ASCPQFAANF TRNAWEF
 
 
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