YHBE_ECOLI
ID YHBE_ECOLI Reviewed; 321 AA.
AC P0AA73; P28636; Q2M928;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2005, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Uncharacterized inner membrane transporter YhbE;
GN Name=yhbE; OrderedLocusNames=b3184, JW3151;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-265.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8312607; DOI=10.3109/10425179309015624;
RA Jeong J.H., Kitakawa M.S., Isono S., Isono K.;
RT "Cloning and nucleotide sequencing of the genes, rpIU and rpmA, for
RT ribosomal proteins L21 and L27 of Escherichia coli.";
RL DNA Seq. 4:59-67(1993).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP DISRUPTION PHENOTYPE.
RC STRAIN=TOP10;
RX PubMed=26051177; DOI=10.1016/j.molcel.2015.05.011;
RA Verstraeten N., Knapen W.J., Kint C.I., Liebens V., Van den Bergh B.,
RA Dewachter L., Michiels J.E., Fu Q., David C.C., Fierro A.C., Marchal K.,
RA Beirlant J., Versees W., Hofkens J., Jansen M., Fauvart M., Michiels J.;
RT "Obg and membrane depolarization are part of a microbial bet-hedging
RT strategy that leads to antibiotic tolerance.";
RL Mol. Cell 59:9-21(2015).
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- DISRUPTION PHENOTYPE: No visible effect on bacterial persistence.
CC {ECO:0000269|PubMed:26051177}.
CC -!- SIMILARITY: Belongs to the EamA transporter family. {ECO:0000305}.
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DR EMBL; U18997; AAA57985.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76216.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77228.1; -; Genomic_DNA.
DR EMBL; D13267; BAA20990.1; -; Genomic_DNA.
DR PIR; B65109; B65109.
DR RefSeq; NP_417651.1; NC_000913.3.
DR RefSeq; WP_000813037.1; NZ_STEB01000012.1.
DR AlphaFoldDB; P0AA73; -.
DR SMR; P0AA73; -.
DR BioGRID; 4259342; 10.
DR STRING; 511145.b3184; -.
DR PaxDb; P0AA73; -.
DR PRIDE; P0AA73; -.
DR EnsemblBacteria; AAC76216; AAC76216; b3184.
DR EnsemblBacteria; BAE77228; BAE77228; BAE77228.
DR GeneID; 66672914; -.
DR GeneID; 947699; -.
DR KEGG; ecj:JW3151; -.
DR KEGG; eco:b3184; -.
DR PATRIC; fig|1411691.4.peg.3547; -.
DR EchoBASE; EB1462; -.
DR eggNOG; COG0697; Bacteria.
DR HOGENOM; CLU_074108_1_0_6; -.
DR InParanoid; P0AA73; -.
DR OMA; FCGANTL; -.
DR PhylomeDB; P0AA73; -.
DR BioCyc; EcoCyc:EG11499-MON; -.
DR PRO; PR:P0AA73; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR InterPro; IPR000620; EamA_dom.
DR Pfam; PF00892; EamA; 2.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Repeat;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..321
FT /note="Uncharacterized inner membrane transporter YhbE"
FT /id="PRO_0000108169"
FT TOPO_DOM 1..5
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 27..35
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 57..70
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..99
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..156
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 178..190
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..216
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..249
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 271..278
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 279..299
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 300..321
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 17..144
FT /note="EamA 1"
FT DOMAIN 169..292
FT /note="EamA 2"
SQ SEQUENCE 321 AA; 34963 MW; 9D1E6F9EE30C9518 CRC64;
MKQQAGIGIL LALTTAICWG ALPIAMKQVL EVMEPPTIVF YRFLMASIGL GAILAVKKRL
PPLRVFRKPR WLILLAVATA GLFGNFILFS SSLQYLSPTA SQVIGQLSPV GMMVASVFIL
KEKMRSTQVV GALMLLSGLV MFFNTSLVEI FTKLTDYTWG VIFGVGAATV WVSYGVAQKV
LLRRLASPQI LFLLYTLCTI ALFPLAKPGV IAQLSHWQLA CLIFCGLNTL VGYGALAEAM
ARWQAAQVSA IITLTPLFTL FFSDLLSLAW PDFFARPMLN LLGYLGAFVV VAGAMYSAIG
HRIWGGLRKH TTVVSQPRAG E
