YHBY_ECOLI
ID YHBY_ECOLI Reviewed; 97 AA.
AC P0AGK4; P42550; Q2M932;
DT 20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=RNA-binding protein YhbY;
GN Name=yhbY; OrderedLocusNames=b3180, JW3147;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Wang R., Kushner S.R.;
RT "Identification and physical analysis of new genes in the argG region (69
RT min) of Escherichia coli chromosome.";
RL Submitted (SEP-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-97.
RX PubMed=2243801; DOI=10.1093/nar/18.21.6443;
RA Sparkowski J., Das A.;
RT "The nucleotide sequence of greA, a suppressor gene that restores growth of
RT an Escherichia coli RNA polymerase mutant at high temperature.";
RL Nucleic Acids Res. 18:6443-6443(1990).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND ASSOCIATION
RP WITH THE 50S RIBOSOMAL SUBUNIT.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=16980477; DOI=10.1128/jb.00444-06;
RA Jiang M., Datta K., Walker A., Strahler J., Bagamasbad P., Andrews P.C.,
RA Maddock J.R.;
RT "The Escherichia coli GTPase CgtAE is involved in late steps of large
RT ribosome assembly.";
RL J. Bacteriol. 188:6757-6770(2006).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 5-104.
RX PubMed=12429100; DOI=10.1016/s0969-2126(02)00886-9;
RA Ostheimer G.J., Barkan A., Matthews B.W.;
RT "Crystal structure of E. coli YhbY: a representative of a novel class of
RT RNA binding proteins.";
RL Structure 10:1593-1601(2002).
CC -!- INTERACTION:
CC P0AGK4; P0AG30: rho; NbExp=3; IntAct=EBI-543346, EBI-545468;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16980477}. Note=Has
CC been seen to comigrate with the 50S ribosomal subunit in sucrose
CC gradients.
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DR EMBL; U01376; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U18997; AAA57981.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76212.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77224.1; -; Genomic_DNA.
DR EMBL; X54718; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; F65108; F65108.
DR RefSeq; NP_417647.1; NC_000913.3.
DR RefSeq; WP_001054420.1; NZ_STEB01000012.1.
DR PDB; 1LN4; X-ray; 1.50 A; A=3-97.
DR PDBsum; 1LN4; -.
DR AlphaFoldDB; P0AGK4; -.
DR SMR; P0AGK4; -.
DR BioGRID; 4261795; 34.
DR BioGRID; 852001; 1.
DR DIP; DIP-47893N; -.
DR IntAct; P0AGK4; 44.
DR STRING; 511145.b3180; -.
DR jPOST; P0AGK4; -.
DR PaxDb; P0AGK4; -.
DR PRIDE; P0AGK4; -.
DR EnsemblBacteria; AAC76212; AAC76212; b3180.
DR EnsemblBacteria; BAE77224; BAE77224; BAE77224.
DR GeneID; 67415986; -.
DR GeneID; 947688; -.
DR KEGG; ecj:JW3147; -.
DR KEGG; eco:b3180; -.
DR PATRIC; fig|1411691.4.peg.3552; -.
DR EchoBASE; EB2646; -.
DR eggNOG; COG1534; Bacteria.
DR HOGENOM; CLU_095994_2_0_6; -.
DR InParanoid; P0AGK4; -.
DR OMA; FQVGKNG; -.
DR PhylomeDB; P0AGK4; -.
DR BioCyc; EcoCyc:EG12794-MON; -.
DR EvolutionaryTrace; P0AGK4; -.
DR PRO; PR:P0AGK4; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:1990275; F:preribosome binding; IDA:EcoCyc.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IMP:EcoCyc.
DR GO; GO:0000028; P:ribosomal small subunit assembly; IMP:EcoCyc.
DR GO; GO:0000967; P:rRNA 5'-end processing; IMP:EcoCyc.
DR Gene3D; 3.30.110.60; -; 1.
DR InterPro; IPR001890; RNA-binding_CRM.
DR InterPro; IPR017924; RNA-binding_YhbY.
DR InterPro; IPR035920; YhbY-like_sf.
DR Pfam; PF01985; CRS1_YhbY; 1.
DR SMART; SM01103; CRS1_YhbY; 1.
DR SUPFAM; SSF75471; SSF75471; 1.
DR TIGRFAMs; TIGR00253; RNA_bind_YhbY; 1.
DR PROSITE; PS51295; CRM; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Reference proteome; RNA-binding.
FT CHAIN 1..97
FT /note="RNA-binding protein YhbY"
FT /id="PRO_0000202163"
FT DOMAIN 1..97
FT /note="CRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00626"
FT HELIX 5..15
FT /evidence="ECO:0007829|PDB:1LN4"
FT STRAND 21..24
FT /evidence="ECO:0007829|PDB:1LN4"
FT HELIX 31..44
FT /evidence="ECO:0007829|PDB:1LN4"
FT STRAND 45..51
FT /evidence="ECO:0007829|PDB:1LN4"
FT HELIX 56..70
FT /evidence="ECO:0007829|PDB:1LN4"
FT STRAND 73..78
FT /evidence="ECO:0007829|PDB:1LN4"
FT STRAND 81..85
FT /evidence="ECO:0007829|PDB:1LN4"
SQ SEQUENCE 97 AA; 10784 MW; 52B4F7AD4C203382 CRC64;
MNLSTKQKQH LKGLAHPLKP VVLLGSNGLT EGVLAEIEQA LEHHELIKVK IATEDRETKT
LIVEAIVRET GACNVQVIGK TLVLYRPTKE RKISLPR
