ID   YHCG_ECOLI              Reviewed;         375 AA.
AC   P45423; Q2M8Z3;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Putative nuclease YhcG {ECO:0000305|PubMed:19402753};
DE            EC=3.1.-.- {ECO:0000305};
GN   Name=yhcG; OrderedLocusNames=b3220, JW3189;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   PREDICTED FUNCTION, AND DOMAIN.
RX   PubMed=16011798; DOI=10.1186/1471-2105-6-172;
RA   Kosinski J., Feder M., Bujnicki J.M.;
RT   "The PD-(D/E)XK superfamily revisited: identification of new members among
RT   proteins involved in DNA metabolism and functional predictions for domains
RT   of (hitherto) unknown function.";
RL   BMC Bioinformatics 6:172-172(2005).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP   INTERACTION WITH DNA PROCESSING ENZYMES.
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=19402753; DOI=10.1371/journal.pbio.1000096;
RA   Hu P., Janga S.C., Babu M., Diaz-Mejia J.J., Butland G., Yang W.,
RA   Pogoutse O., Guo X., Phanse S., Wong P., Chandran S., Christopoulos C.,
RA   Nazarians-Armavil A., Nasseri N.K., Musso G., Ali M., Nazemof N.,
RA   Eroukova V., Golshani A., Paccanaro A., Greenblatt J.F.,
RA   Moreno-Hagelsieb G., Emili A.;
RT   "Global functional atlas of Escherichia coli encompassing previously
RT   uncharacterized proteins.";
RL   PLoS Biol. 7:E96-E96(2009).
CC   -!- FUNCTION: May be a nuclease involved in DNA recombination and repair.
CC       {ECO:0000305|PubMed:16011798, ECO:0000305|PubMed:19402753}.
CC   -!- SUBUNIT: Interacts with DNA processing enzymes, including the
CC       restriction complex HsdMRS, the integrases IntF and IntS, and the
CC       recombinase PinE. {ECO:0000269|PubMed:19402753}.
CC   -!- DOMAIN: Possesses a PD-(D/E)XK-like nuclease domain.
CC       {ECO:0000305|PubMed:16011798}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of this gene results in a synthetic-
CC       lethal phenotype when combined with hypomorphic alleles of the
CC       replicative primosome (dnaB), DNA polymerase III (dnaN), and DNA
CC       topoisomerase IV (parE). {ECO:0000269|PubMed:19402753}.
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DR   EMBL; U18997; AAA58022.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76252.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77263.1; -; Genomic_DNA.
DR   PIR; F65113; F65113.
DR   RefSeq; NP_417687.1; NC_000913.3.
DR   RefSeq; WP_000445111.1; NZ_LN832404.1.
DR   AlphaFoldDB; P45423; -.
DR   SMR; P45423; -.
DR   BioGRID; 4262441; 20.
DR   IntAct; P45423; 1.
DR   STRING; 511145.b3220; -.
DR   PaxDb; P45423; -.
DR   PRIDE; P45423; -.
DR   EnsemblBacteria; AAC76252; AAC76252; b3220.
DR   EnsemblBacteria; BAE77263; BAE77263; BAE77263.
DR   GeneID; 947739; -.
DR   KEGG; ecj:JW3189; -.
DR   KEGG; eco:b3220; -.
DR   PATRIC; fig|511145.12.peg.3316; -.
DR   EchoBASE; EB2664; -.
DR   eggNOG; COG4804; Bacteria.
DR   HOGENOM; CLU_046640_1_1_6; -.
DR   InParanoid; P45423; -.
DR   OMA; LTHQDVG; -.
DR   PhylomeDB; P45423; -.
DR   BioCyc; EcoCyc:G7674-MON; -.
DR   PRO; PR:P45423; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.1350.10; -; 1.
DR   InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR   InterPro; IPR009362; YhcG_C.
DR   InterPro; IPR041527; YhcG_N.
DR   Pfam; PF17761; DUF1016_N; 1.
DR   Pfam; PF06250; YhcG_C; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Hydrolase; Nuclease; Reference proteome.
FT   CHAIN           1..375
FT                   /note="Putative nuclease YhcG"
FT                   /id="PRO_0000169484"
SQ   SEQUENCE   375 AA;  43293 MW;  76F67ABCDA815639 CRC64;
     MESLSEGTTA GYQQIHDGII HLVDSARTET VRSVNALMTA TYQEIGRRIV EFEQGGEARA
     AYGAQLIKRL SKDLCLRYKR GFSAKNLRQM RLFYLFFQHV EIHQTMSGEL TPLGIPQTPS
     AEFPSAKIWQ TLSAKSFPLP RSTYVRLLSV KNADARSFYE KETLRCGWSV RQLERQIATQ
     FYERTLLSHD KSAMLQQHAP AETHILPQQA IRDPFVLEFL ELKDEYSESD FEEALINHLM
     DFMLELGDDF AFVGRQRRLR IDDNWFRVDL LFFHRRLRCL LIVDLKVGKF SYSDAGQMNM
     YLNYAKEHWT LPDENPPIGL VLCAEKGAGE AHYALAGLPN TVLASEYKMQ LPDEKRLADE
     LVRTQAVLEE GYRRR