ID   YHCX_BACSU              Reviewed;         513 AA.
AC   P54608;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 124.
DE   RecName: Full=Hydrolase YhcX;
DE            EC=3.5.-.-;
GN   Name=yhcX; OrderedLocusNames=BSU09250;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=8969498; DOI=10.1099/13500872-142-11-3021;
RA   Noback M.A., Terpstra P., Holsappel S., Venema G., Bron S.;
RT   "A 22 kb DNA sequence in the cspB-glpPFKD region at 75 degrees on the
RT   Bacillus subtilis chromosome.";
RL   Microbiology 142:3021-3026(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   SEQUENCE REVISION TO 137.
RX   PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA   Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA   Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT   "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT   168 reference genome a decade later.";
RL   Microbiology 155:1758-1775(2009).
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       NIT1/NIT2 family. {ECO:0000305}.
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DR   EMBL; X96983; CAA65708.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB12753.2; -; Genomic_DNA.
DR   PIR; D69824; D69824.
DR   RefSeq; NP_388806.2; NC_000964.3.
DR   RefSeq; WP_003245514.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P54608; -.
DR   SMR; P54608; -.
DR   STRING; 224308.BSU09250; -.
DR   jPOST; P54608; -.
DR   PaxDb; P54608; -.
DR   PRIDE; P54608; -.
DR   EnsemblBacteria; CAB12753; CAB12753; BSU_09250.
DR   GeneID; 939265; -.
DR   KEGG; bsu:BSU09250; -.
DR   PATRIC; fig|224308.179.peg.997; -.
DR   eggNOG; COG0388; Bacteria.
DR   eggNOG; COG3153; Bacteria.
DR   InParanoid; P54608; -.
DR   OMA; GLIQWQM; -.
DR   PhylomeDB; P54608; -.
DR   BioCyc; BSUB:BSU09250-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR000182; GNAT_dom.
DR   InterPro; IPR001110; UPF0012_CS.
DR   Pfam; PF00583; Acetyltransf_1; 1.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF55729; SSF55729; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS51186; GNAT; 1.
DR   PROSITE; PS01227; UPF0012; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..513
FT                   /note="Hydrolase YhcX"
FT                   /id="PRO_0000213260"
FT   DOMAIN          14..212
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT   DOMAIN          229..484
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        270
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        345
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        379
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   CONFLICT        137
FT                   /note="N -> S (in Ref. 1; CAA65708)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   513 AA;  60218 MW;  6462F5AA4B67AAD4 CRC64;
     MSEKLDLTRF EKKMVIRNIE EKDIDKIIDL QKDCFPGMEP WKREHLISHL EHFPEGQFCA
     EFEGEIIGSC SSLLINFDEY DDRHTWQDIT DDGYITNHNP DGLNMYGIEV MVHPKYRRMK
     IGHRLYEARK DLARRLNLKS IIIGGRIPNY HKYAEEMTAR EYVEQVTRHQ IYDPVLSFQL
     MNGFTLMRIN PNYLPDDTAS IKYATLMEWN NVDYLPQQTK RYYKSAFPVR ICVIQYEMKK
     IYSFEEFANQ VEYYVDVASD ARSDFAVFPE IFTTQLMSFL EERSPSLAVQ RITEYTEDYI
     SLFTDLAVKY NVNIIGGSHF VEEEGKIYNI AYLFRRDGTI EKQYKLHITP NERKWWGISA
     GDQVRVFDTD CGKIAIQICY DIEFPELARI AADKGAKIIF TPFCTEDRQG YLRVRYCSQA
     RAVENQIYTV ISGTVGNLPQ TENMDIQYAQ SGIFAPSDFE FARDGIVGET NPNIEMVVIG
     DVDLEILRRQ RQNGTVRQLK DRRRDIYHIQ YKK