YHDY_BACSU
ID YHDY_BACSU Reviewed; 371 AA.
AC O07594;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Uncharacterized MscS family protein YhdY;
GN Name=yhdY; OrderedLocusNames=BSU09640;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA Venema G., Bron S.;
RT "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL Microbiology 144:859-875(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=168 / JH642;
RX PubMed=18310427; DOI=10.1128/aem.01573-07;
RA Hoffmann T., Boiangiu C., Moses S., Bremer E.;
RT "Responses of Bacillus subtilis to hypotonic challenges: physiological
RT contributions of mechanosensitive channels to cellular survival.";
RL Appl. Environ. Microbiol. 74:2454-2460(2008).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168 / PS832;
RX PubMed=17665170; DOI=10.1007/s00203-007-0292-z;
RA Wahome P.G., Setlow P.;
RT "Growth, osmotic downshock resistance and differentiation of Bacillus
RT subtilis strains lacking mechanosensitive channels.";
RL Arch. Microbiol. 189:49-58(2008).
CC -!- FUNCTION: May play a role in resistance to osmotic downshock.
CC {ECO:0000269|PubMed:17665170}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene grow normally in minimal
CC medium or in high-osmolarity environments, and exhibit the same
CC survival capacity as the wild-type strain upon a drastic osmotic
CC downshift. They sporulate and germinate normally.
CC {ECO:0000269|PubMed:17665170, ECO:0000269|PubMed:18310427}.
CC -!- SIMILARITY: Belongs to the MscS (TC 1.A.23) family. {ECO:0000305}.
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DR EMBL; Y14082; CAA74509.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12803.1; -; Genomic_DNA.
DR PIR; G69827; G69827.
DR RefSeq; NP_388845.1; NC_000964.3.
DR RefSeq; WP_003245529.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; O07594; -.
DR SMR; O07594; -.
DR STRING; 224308.BSU09640; -.
DR PaxDb; O07594; -.
DR PRIDE; O07594; -.
DR EnsemblBacteria; CAB12803; CAB12803; BSU_09640.
DR GeneID; 939285; -.
DR KEGG; bsu:BSU09640; -.
DR PATRIC; fig|224308.179.peg.1037; -.
DR eggNOG; COG0668; Bacteria.
DR InParanoid; O07594; -.
DR OMA; NIEFAFP; -.
DR PhylomeDB; O07594; -.
DR BioCyc; BSUB:BSU09640-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR Gene3D; 2.30.30.60; -; 1.
DR InterPro; IPR010920; LSM_dom_sf.
DR InterPro; IPR006685; MscS_channel.
DR InterPro; IPR011066; MscS_channel_C.
DR InterPro; IPR006686; MscS_channel_CS.
DR InterPro; IPR011014; MscS_channel_TM-2.
DR InterPro; IPR023408; MscS_dom_sf.
DR InterPro; IPR045042; YnaI-like.
DR PANTHER; PTHR43634; PTHR43634; 1.
DR Pfam; PF00924; MS_channel; 1.
DR SUPFAM; SSF50182; SSF50182; 1.
DR SUPFAM; SSF82689; SSF82689; 1.
DR SUPFAM; SSF82861; SSF82861; 1.
DR PROSITE; PS01246; UPF0003; 1.
PE 3: Inferred from homology;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..371
FT /note="Uncharacterized MscS family protein YhdY"
FT /id="PRO_0000110245"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 183..203
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 371 AA; 42537 MW; ABE76B77EBC2D9B7 CRC64;
MPDYLQQYFT LDNIIQIGIS LAILLVFLIL RKLFTRYFFN LLFNLTNRPK TEIFKQVVLA
FDKPARWFFV ALGLFLAIRY SPFLDEQMPV ISKIYRSLIV ALLCWGLCNL TATSSFIFHK
VNQRFELDMD DILAPFLSKL LRFVIIALSV SVIAQEFNYD VNGFVAGLGL GGLAFALAAK
DTISNFFGGI IIITEKPFTI GDWVETSTVT GSVEDITFRS TRFRTAQGAL VTVPNSTLSM
EAITNWTRMT KRQITFSIHV SYATPIENLE RSIHSLRTML LEHEGVDNEI IMVNFDTFAD
SYYNLFFNFY TKTTVWAENL NIREDINYKI IEILGAEGVQ FAYPGQMVVV KQKHESDQFQ
VNLNKEEKER A