YHE4_YEAST
ID YHE4_YEAST Reviewed; 235 AA.
AC P38727; D3DKS6; Q70D60; Q70DF5; Q70DF7; Q70DF9; Q70DG3; Q70DG4; Q70DG5;
AC Q70DG6; Q70DG7;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=DUP240 protein YHL044W;
GN OrderedLocusNames=YHL044W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS ASP-9; LEU-47; VAL-75;
RP PRO-86; ASN-96; THR-103; VAL-124; MET-145; SER-157; ARG-160; ASP-180;
RP ALA-189; ASP-233 AND ILE-234.
RC STRAIN=CLIB 219, CLIB 382, CLIB 388, CLIB 410, CLIB 413, CLIB 556,
RC CLIB 630, CLIB 95, K1, R12, R13, Sigma 1278B, YIIc12, and YIIc17;
RX PubMed=15087486; DOI=10.1093/nar/gkh529;
RA Leh-Louis V., Wirth B., Despons L., Wain-Hobson S., Potier S.,
RA Souciet J.-L.;
RT "Differential evolution of the Saccharomyces cerevisiae DUP240 paralogs and
RT implication of recombination in phylogeny.";
RL Nucleic Acids Res. 32:2069-2078(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP INTERACTION WITH BZZ1; SRB4 AND SUA7.
RX PubMed=11283351; DOI=10.1073/pnas.061034498;
RA Ito T., Chiba T., Ozawa R., Yoshida M., Hattori M., Sakaki Y.;
RT "A comprehensive two-hybrid analysis to explore the yeast protein
RT interactome.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:4569-4574(2001).
RN [5]
RP SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=12101299; DOI=10.1099/00221287-148-7-2111;
RA Poirey R., Despons L., Leh V., Lafuente M.-J., Potier S., Souciet J.-L.,
RA Jauniaux J.-C.;
RT "Functional analysis of the Saccharomyces cerevisiae DUP240 multigene
RT family reveals membrane-associated proteins that are not essential for cell
RT viability.";
RL Microbiology 148:2111-2123(2002).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- SUBUNIT: Interacts according to large scale protein interaction studies
CC with BZZ1, SRB4 and SUA7. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12101299};
CC Multi-pass membrane protein {ECO:0000269|PubMed:12101299}.
CC -!- DISRUPTION PHENOTYPE: Members of the DUP240 multigene family are
CC specific to S.cerevisiae sensu strictu. Cells lacking all 10 DUP240
CC proteins show no obvious alterations in mating, sporulation and cell
CC growth. {ECO:0000269|PubMed:12101299}.
CC -!- SIMILARITY: Belongs to the DUP/COS family. {ECO:0000305}.
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DR EMBL; AJ585565; CAE52085.1; -; Genomic_DNA.
DR EMBL; AJ585566; CAE52086.1; -; Genomic_DNA.
DR EMBL; AJ585567; CAE52087.1; -; Genomic_DNA.
DR EMBL; AJ585568; CAE52088.1; -; Genomic_DNA.
DR EMBL; AJ585569; CAE52089.1; -; Genomic_DNA.
DR EMBL; AJ585570; CAE52090.1; -; Genomic_DNA.
DR EMBL; AJ585571; CAE52091.1; -; Genomic_DNA.
DR EMBL; AJ585572; CAE52092.1; -; Genomic_DNA.
DR EMBL; AJ585573; CAE52093.1; -; Genomic_DNA.
DR EMBL; AJ585574; CAE52094.1; -; Genomic_DNA.
DR EMBL; AJ585575; CAE52095.1; -; Genomic_DNA.
DR EMBL; AJ585576; CAE52096.1; -; Genomic_DNA.
DR EMBL; AJ585577; CAE52097.1; -; Genomic_DNA.
DR EMBL; AJ585578; CAE52098.1; -; Genomic_DNA.
DR EMBL; AJ585579; CAE52099.1; -; Genomic_DNA.
DR EMBL; AJ585580; CAE52100.1; -; Genomic_DNA.
DR EMBL; AJ585581; CAE52101.1; -; Genomic_DNA.
DR EMBL; AJ585582; CAE52102.1; -; Genomic_DNA.
DR EMBL; AJ585755; CAE52275.1; -; Genomic_DNA.
DR EMBL; U11583; AAB65056.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06643.1; -; Genomic_DNA.
DR PIR; S48924; S48924.
DR RefSeq; NP_011819.1; NM_001179124.1.
DR AlphaFoldDB; P38727; -.
DR BioGRID; 36380; 18.
DR DIP; DIP-2089N; -.
DR STRING; 4932.YHL044W; -.
DR PaxDb; P38727; -.
DR PRIDE; P38727; -.
DR EnsemblFungi; YHL044W_mRNA; YHL044W; YHL044W.
DR GeneID; 856341; -.
DR KEGG; sce:YHL044W; -.
DR SGD; S000001036; YHL044W.
DR VEuPathDB; FungiDB:YHL044W; -.
DR GeneTree; ENSGT00940000176285; -.
DR HOGENOM; CLU_081384_1_0_1; -.
DR InParanoid; P38727; -.
DR OMA; AEVITHK; -.
DR BioCyc; YEAST:G3O-31061-MON; -.
DR PRO; PR:P38727; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38727; protein.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IMP:SGD.
DR InterPro; IPR001142; DUP/COS.
DR Pfam; PF00674; DUP; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..235
FT /note="DUP240 protein YHL044W"
FT /id="PRO_0000207533"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..72
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 94..235
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 9
FT /note="N -> D (in strain: CLIB 95, CLIB 219, CLIB 382, CLIB
FT 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13,
FT YIIc12 and YIIc17)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 47
FT /note="F -> L (in strain: CLIB 219, CLIB 388, CLIB 410,
FT CLIB 413, CLIB 556, CLIB 630, YIIc12 haplotype Ha2 and
FT YIIc17 haplotype Ha2)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 75
FT /note="I -> V (in strain: CLIB 95, CLIB 382, CLIB 556
FT haplotype Ha1, K1, R12, R13, YIIc12 haplotype Ha1 and
FT YIIc17 haplotype Ha1)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 77
FT /note="L -> F (in strain: CLIB 388)"
FT VARIANT 86
FT /note="L -> P (in strain: CLIB 388)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 96
FT /note="S -> N (in strain: CLIB 95, CLIB 219, CLIB 382, CLIB
FT 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13,
FT YIIc12 and YIIc17)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 103
FT /note="R -> T (in strain: CLIB 388, CLIB 410, CLIB 556
FT haplotype Ha2, CLIB 630, YIIc12 haplotype Ha2 and YIIc17
FT haplotype Ha2)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 124
FT /note="M -> V (in strain: CLIB 95, CLIB 219, CLIB 382, CLIB
FT 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13,
FT YIIc12 and YIIc17)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 145
FT /note="I -> M (in strain: R12 haplotype Ha2)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 157
FT /note="Y -> S (in strain: CLIB 95, CLIB 219, CLIB 382, CLIB
FT 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13,
FT YIIc12 and YIIc17)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 160
FT /note="Q -> R (in strain: CLIB 556 haplotype Ha1)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 180
FT /note="Y -> D (in strain: CLIB 95, CLIB 382, K1, R12, R13,
FT YIIc12 haplotype Ha1 and YIIc17 haplotype Ha1)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 189
FT /note="G -> A (in strain: CLIB 95, CLIB 219, CLIB 382, CLIB
FT 388, CLIB 410, CLIB 413, CLIB 556, CLIB 630, K1, R12, R13,
FT YIIc12 and YIIc17)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 225..235
FT /note="EDKYPEMGVTV -> RISIQRWGTQF (in strain: CLIB 630)"
FT VARIANT 233
FT /note="V -> D (in strain: CLIB 219, CLIB 388, CLIB 410,
FT CLIB 413, CLIB 556, CLIB 630, YIIc12 haplotype Ha2 and
FT YIIc17 haplotype Ha2)"
FT /evidence="ECO:0000269|PubMed:15087486"
FT VARIANT 234
FT /note="T -> I (in strain: CLIB 413 haplotype Ha1)"
FT /evidence="ECO:0000269|PubMed:15087486"
SQ SEQUENCE 235 AA; 26972 MW; 2A789B602087D928 CRC64;
MSSELLISNS KPRPEGLRKL CEGETVILPR DITPSKCAYF LKQNIVFISY IFIHIIITII
LNRLALSAHG NTLIIILAAL LITISLFLLL LLPYLSCSRY KLRCLDDDCK FKLLAEVITH
KPNMDLSTWD RIAYDMNQFV YDRRICADRS FFYDGSYCYQ VFKKLVATPY LVNSNMNSIY
ADLEMRSNGA TNINDSGNSS LHIELGTYIF KALAVFRNSV DKYWEDKYPE MGVTV
