ID   CBIB_SALTY              Reviewed;         319 AA.
AC   Q05600;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Cobalamin biosynthesis protein CbiB;
GN   Name=cbiB; OrderedLocusNames=STM2034;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=LT2;
RX   PubMed=8501034; DOI=10.1128/jb.175.11.3303-3316.1993;
RA   Roth J.R., Lawrence J.G., Rubenfield M., Kieffer-Higgins S., Church G.M.;
RT   "Characterization of the cobalamin (vitamin B12) biosynthetic genes of
RT   Salmonella typhimurium.";
RL   J. Bacteriol. 175:3303-3316(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [3]
RP   POSSIBLE COSUBSTRATE.
RC   STRAIN=LT2;
RX   PubMed=9446573; DOI=10.1074/jbc.273.5.2684;
RA   Brushaber K.R., O'Toole G.A., Escalante-Semerena J.C.;
RT   "CobD, a novel enzyme with L-threonine-O-3-phosphate decarboxylase
RT   activity, is responsible for the synthesis of (R)-1-amino-2-propanol O-2-
RT   phosphate, a proposed new intermediate in cobalamin biosynthesis in
RT   Salmonella typhimurium LT2.";
RL   J. Biol. Chem. 273:2684-2691(1998).
CC   -!- FUNCTION: Converts cobyric acid to cobinamide by the addition of
CC       aminopropanol on the F carboxylic group. However, the true cosubstrate
CC       could be (R)-1-amino-2-propanol O-2-phosphate, leading to cobinamide
CC       phosphate.
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       adenosylcobalamin from cob(II)yrinate a,c-diamide: step 4/7.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CobD/CbiB family. {ECO:0000305}.
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DR   EMBL; L12006; AAA27253.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL20938.1; -; Genomic_DNA.
DR   RefSeq; NP_460979.1; NC_003197.2.
DR   RefSeq; WP_000153648.1; NC_003197.2.
DR   AlphaFoldDB; Q05600; -.
DR   STRING; 99287.STM2034; -.
DR   PaxDb; Q05600; -.
DR   EnsemblBacteria; AAL20938; AAL20938; STM2034.
DR   GeneID; 1253555; -.
DR   KEGG; stm:STM2034; -.
DR   PATRIC; fig|99287.12.peg.2156; -.
DR   HOGENOM; CLU_054212_0_0_6; -.
DR   OMA; WGYRNER; -.
DR   PhylomeDB; Q05600; -.
DR   BioCyc; MetaCyc:MON-13228; -.
DR   BioCyc; SENT99287:STM2034-MON; -.
DR   BRENDA; 6.3.1.10; 2169.
DR   UniPathway; UPA00148; UER00235.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015420; F:ABC-type vitamin B12 transporter activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0048472; F:threonine-phosphate decarboxylase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00024; CobD_CbiB; 1.
DR   InterPro; IPR004485; Cobalamin_biosynth_CobD/CbiB.
DR   PANTHER; PTHR34308; PTHR34308; 1.
DR   Pfam; PF03186; CobD_Cbib; 1.
DR   TIGRFAMs; TIGR00380; cobD; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Cobalamin biosynthesis; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..319
FT                   /note="Cobalamin biosynthesis protein CbiB"
FT                   /id="PRO_0000150937"
FT   TRANSMEM        3..23
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        56..76
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        82..102
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        153..173
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        296..316
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   319 AA;  35370 MW;  540FDF93E0796214 CRC64;
     MTILAWCIAW VLDFIIGDPQ HWPHPVRWIG RLITFVQRIV RRYCPGDKAL RIGGGVMWVV
     VVGATWGVAW GVLALAQRIH PWFGWSVEVW MIFTTLAGRS LAHAAQEVER PLRKNDLAES
     RIKLSWIVGR DTSQLQPAQI NRAVVETVAE NTVDGIIAPL FFLFLGGAPL AMAYKAVNTL
     DSMVGYKHEK YRAIGMVSAR MDDVANYLPA RLSWLLLGIA AGLCRLSGWR ALRIGWRDRY
     NHSSPNCAWS EACVAGALGI QLGGPNNYFG ERVDKPWIGD AQRDISVDDI SRTIRLMWVA
     STLALALLIA ARCGLSGVA