ID   YHFL_ECOLI              Reviewed;          55 AA.
AC   P64627; P45538; Q2M735;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Uncharacterized protein YhfL;
GN   Name=yhfL; OrderedLocusNames=b3369, JW3332;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [3]
RP   POSSIBLE LIPOPROTEIN.
RX   PubMed=12876315; DOI=10.1110/ps.0303703;
RA   Juncker A.S., Willenbrock H., Von Heijne G., Brunak S., Nielsen H.,
RA   Krogh A.;
RT   "Prediction of lipoprotein signal peptides in Gram-negative bacteria.";
RL   Protein Sci. 12:1652-1662(2003).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}. Note=Has been predicted to be a lipoprotein, the
CC       lipid is predicted to attach to Cys-20 (PubMed:12876315).
CC       {ECO:0000305|PubMed:12876315}.
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DR   EMBL; U18997; AAA58166.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76394.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77921.1; -; Genomic_DNA.
DR   PIR; D65131; D65131.
DR   RefSeq; NP_417828.1; NC_000913.3.
DR   RefSeq; WP_001031834.1; NZ_STEB01000004.1.
DR   AlphaFoldDB; P64627; -.
DR   BioGRID; 4263103; 3.
DR   STRING; 511145.b3369; -.
DR   PaxDb; P64627; -.
DR   PRIDE; P64627; -.
DR   EnsemblBacteria; AAC76394; AAC76394; b3369.
DR   EnsemblBacteria; BAE77921; BAE77921; BAE77921.
DR   GeneID; 66672750; -.
DR   GeneID; 947879; -.
DR   KEGG; ecj:JW3332; -.
DR   KEGG; eco:b3369; -.
DR   PATRIC; fig|1411691.4.peg.3360; -.
DR   EchoBASE; EB2744; -.
DR   eggNOG; ENOG5032ZRK; Bacteria.
DR   HOGENOM; CLU_197401_0_0_6; -.
DR   OMA; NKTCSYD; -.
DR   PhylomeDB; P64627; -.
DR   BioCyc; EcoCyc:G7722-MON; -.
DR   PRO; PR:P64627; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR025318; DUF4223.
DR   Pfam; PF13978; DUF4223; 1.
PE   4: Predicted;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..55
FT                   /note="Uncharacterized protein YhfL"
FT                   /id="PRO_0000169524"
FT   TRANSMEM        7..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   55 AA;  5819 MW;  E50D5E046F00A6EE CRC64;
     MNKFIKVALV GAVLATLTAC TGHIENRDKN CSYDYLLHPA ISISKIIGGC GPTAQ