YHFN_BACSU
ID YHFN_BACSU Reviewed; 426 AA.
AC P40769; O07612;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Uncharacterized metalloprotease YhfN;
DE EC=3.4.24.-;
DE AltName: Full=PSP23;
GN Name=yhfN; Synonyms=yzoA; OrderedLocusNames=BSU10290;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9579061; DOI=10.1099/00221287-144-4-859;
RA Noback M.A., Holsappel S., Kiewiet R., Terpstra P., Wambutt R., Wedler H.,
RA Venema G., Bron S.;
RT "The 172 kb prkA-addAB region from 83 degrees to 97 degrees of the Bacillus
RT subtilis chromosome contains several dysfunctional genes, the glyB marker,
RT many genes encoding transporter proteins, and the ubiquitous hit gene.";
RL Microbiology 144:859-875(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-49.
RX PubMed=3145906; DOI=10.1016/0378-1119(88)90207-7;
RA Smith H., de Jong A., Bron S., Venema G.;
RT "Characterization of signal-sequence-coding regions selected from the
RT Bacillus subtilis chromosome.";
RL Gene 70:351-361(1988).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000305};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000305};
CC -!- SIMILARITY: Belongs to the peptidase M48B family. {ECO:0000305}.
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DR EMBL; Y14083; CAA74535.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12869.1; -; Genomic_DNA.
DR EMBL; M22914; AAA22830.1; -; Genomic_DNA.
DR PIR; C69831; C69831.
DR RefSeq; NP_388910.1; NC_000964.3.
DR RefSeq; WP_003233175.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P40769; -.
DR SMR; P40769; -.
DR STRING; 224308.BSU10290; -.
DR MEROPS; M48.009; -.
DR PaxDb; P40769; -.
DR PRIDE; P40769; -.
DR EnsemblBacteria; CAB12869; CAB12869; BSU_10290.
DR GeneID; 936314; -.
DR KEGG; bsu:BSU10290; -.
DR PATRIC; fig|224308.179.peg.1105; -.
DR eggNOG; COG0501; Bacteria.
DR InParanoid; P40769; -.
DR OMA; LPFKIYK; -.
DR BioCyc; BSUB:BSU10290-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004222; F:metalloendopeptidase activity; IBA:GO_Central.
DR GO; GO:0071586; P:CAAX-box protein processing; IBA:GO_Central.
DR CDD; cd07343; M48A_Zmpste24p_like; 1.
DR InterPro; IPR027057; CAXX_Prtase_1.
DR InterPro; IPR001915; Peptidase_M48.
DR InterPro; IPR032456; Peptidase_M48_N.
DR Pfam; PF01435; Peptidase_M48; 1.
DR Pfam; PF16491; Peptidase_M48_N; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 3: Inferred from homology;
KW Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW Zinc.
FT CHAIN 1..426
FT /note="Uncharacterized metalloprotease YhfN"
FT /id="PRO_0000138936"
FT ACT_SITE 278
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 277
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 281
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 357
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT CONFLICT 43..49
FT /note="ASFMKPS -> LESTAQA (in Ref. 3; AAA22830)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 426 AA; 48875 MW; 0F69E7EFAA8A0143 CRC64;
MRKWIAAAGL AYVLYGLFFY WYFFLSGDSA IPEAVKGTQA DPASFMKPSE LAVAEQYSNV
KNFLFFIGVP LDWFLFFVLL VSGVSKKIKK WIEAAVPFRF LQTVGFVFVL SLITTLVTLP
LDWIGYQVSL DYNISTQTTA SWAKDQVISF WISFPIFTLC VLVFYWLIKR HEKKWWLYAW
LLTVPFSLFL FFIQPVIIDP LYNDFYPLKN KELESKILEL ADEANIPADH VYEVNMSEKT
NALNAYVTGI GANKRIVLWD TTLNKLDDSE ILFIMGHEMG HYVMKHVYIG LAGYLLVSLA
GFYVIDKLYK RTVRLTRSMF HLEGRHDLAA LPLLLLLFSV LSFAVTPFSN AVSRYQENKA
DQYGIELTEN REAAVKTFQD LAVTGLSQVD PPVLVKIFRG SHPSIMERIQ HAEKEENAPE
HQDADK
