YHHS_ECOLI
ID YHHS_ECOLI Reviewed; 405 AA.
AC P37621; Q2M7D6;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Uncharacterized MFS-type transporter YhhS {ECO:0000255|HAMAP-Rule:MF_01118, ECO:0000305};
GN Name=yhhS; OrderedLocusNames=b3473, JW5945;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP OVEREXPRESSION.
RX PubMed=22089966; DOI=10.1007/s10295-011-1057-x;
RA Staub J.M., Brand L., Tran M., Kong Y., Rogers S.G.;
RT "Bacterial glyphosate resistance conferred by overexpression of an E. coli
RT membrane efflux transporter.";
RL J. Ind. Microbiol. Biotechnol. 39:641-647(2012).
RN [6]
RP OVEREXPRESSION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22952739; DOI=10.1371/journal.pone.0043700;
RA Koita K., Rao C.V.;
RT "Identification and analysis of the putative pentose sugar efflux
RT transporters in Escherichia coli.";
RL PLoS ONE 7:E43700-E43700(2012).
CC -!- FUNCTION: Confers high-level resistance to glyphosate when
CC overexpressed (PubMed:22089966). Overexpression has no effect on
CC intracellular arabinose concentrations (PubMed:22952739).
CC {ECO:0000269|PubMed:22089966, ECO:0000269|PubMed:22952739}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01118, ECO:0000269|PubMed:15919996}; Multi-pass membrane
CC protein {ECO:0000255|HAMAP-Rule:MF_01118, ECO:0000305|PubMed:15919996}.
CC -!- INDUCTION: Induced by arabinose. {ECO:0000269|PubMed:22952739}.
CC -!- DISRUPTION PHENOTYPE: Deletion increases intracellular arabinose
CC concentrations as compared to the wild type.
CC {ECO:0000269|PubMed:22952739}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. YhhS family.
CC {ECO:0000255|HAMAP-Rule:MF_01118, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB18448.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U00039; AAB18448.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC76498.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE77820.1; -; Genomic_DNA.
DR PIR; S47692; S47692.
DR RefSeq; NP_417930.2; NC_000913.3.
DR RefSeq; WP_001300943.1; NZ_SSZK01000008.1.
DR AlphaFoldDB; P37621; -.
DR SMR; P37621; -.
DR BioGRID; 4263334; 147.
DR STRING; 511145.b3473; -.
DR TCDB; 2.A.1.46.7; the major facilitator superfamily (mfs).
DR PaxDb; P37621; -.
DR PRIDE; P37621; -.
DR EnsemblBacteria; AAC76498; AAC76498; b3473.
DR EnsemblBacteria; BAE77820; BAE77820; BAE77820.
DR GeneID; 947982; -.
DR KEGG; ecj:JW5945; -.
DR KEGG; eco:b3473; -.
DR PATRIC; fig|511145.12.peg.3572; -.
DR EchoBASE; EB2133; -.
DR eggNOG; COG0477; Bacteria.
DR HOGENOM; CLU_001265_10_3_6; -.
DR InParanoid; P37621; -.
DR OMA; CAFVGAR; -.
DR PhylomeDB; P37621; -.
DR BioCyc; EcoCyc:YHHS-MON; -.
DR PRO; PR:P37621; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR Gene3D; 1.20.1250.20; -; 1.
DR HAMAP; MF_01118; MFS_YhhS; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR023008; MFS_YhhS-like.
DR Pfam; PF07690; MFS_1; 2.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..405
FT /note="Uncharacterized MFS-type transporter YhhS"
FT /id="PRO_0000087807"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 19..39
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 40..46
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 47..67
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 68..84
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 106
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 128..155
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 177
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 199..223
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 224..244
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 245..251
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 273..282
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 283..303
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 304..308
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 330..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 365
FT /note="Periplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01118"
FT TOPO_DOM 387..405
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:15919996"
SQ SEQUENCE 405 AA; 42156 MW; 4FC17F42FFAE51D1 CRC64;
MPEPVAEPAL NGLRLNLRIV SIVMFNFASY LTIGLPLAVL PGYVHDVMGF SAFWAGLVIS
LQYFATLLSR PHAGRYADSL GPKKIVVFGL CGCFLSGLGY LTAGLTASLP VISLLLLCLG
RVILGIGQSF AGTGSTLWGV GVVGSLHIGR VISWNGIVTY GAMAMGAPLG VVFYHWGGLQ
ALALIIMGVA LVAILLAIPR PTVKASKGKP LPFRAVLGRV WLYGMALALA SAGFGVIATF
ITLFYDAKGW DGAAFALTLF SCAFVGTRLL FPNGINRIGG LNVAMICFSV EIIGLLLVGV
ATMPWMAKIG VLLAGAGFSL VFPALGVVAV KAVPQQNQGA ALATYTVFMD LSLGVTGPLA
GLVMSWAGVP VIYLAAAGLV AIALLLTWRL KKRPPEHVPE AASSS
