YHJ3_YEAST
ID YHJ3_YEAST Reviewed; 423 AA.
AC P38690; D3DKY0; Q66R86;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=Uncharacterized protein YHR033W;
GN OrderedLocusNames=YHR033W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=11914276; DOI=10.1101/gad.970902;
RA Kumar A., Agarwal S., Heyman J.A., Matson S., Heidtman M., Piccirillo S.,
RA Umansky L., Drawid A., Jansen R., Liu Y., Cheung K.-H., Miller P.,
RA Gerstein M., Roeder G.S., Snyder M.;
RT "Subcellular localization of the yeast proteome.";
RL Genes Dev. 16:707-719(2002).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11914276}.
CC -!- SIMILARITY: Belongs to the glutamate 5-kinase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U00062; AAB68910.1; -; Genomic_DNA.
DR EMBL; AY723823; AAU09740.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06724.1; -; Genomic_DNA.
DR PIR; S46741; S46741.
DR RefSeq; NP_011898.1; NM_001179163.1.
DR AlphaFoldDB; P38690; -.
DR SMR; P38690; -.
DR BioGRID; 36464; 62.
DR DIP; DIP-5036N; -.
DR IntAct; P38690; 21.
DR MINT; P38690; -.
DR STRING; 4932.YHR033W; -.
DR MaxQB; P38690; -.
DR PaxDb; P38690; -.
DR PRIDE; P38690; -.
DR EnsemblFungi; YHR033W_mRNA; YHR033W; YHR033W.
DR GeneID; 856428; -.
DR KEGG; sce:YHR033W; -.
DR SGD; S000001075; YHR033W.
DR VEuPathDB; FungiDB:YHR033W; -.
DR eggNOG; KOG1154; Eukaryota.
DR GeneTree; ENSGT00500000044903; -.
DR HOGENOM; CLU_025400_1_1_1; -.
DR InParanoid; P38690; -.
DR OMA; VSEYVAH; -.
DR BioCyc; YEAST:G3O-31093-MON; -.
DR PRO; PR:P38690; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38690; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004349; F:glutamate 5-kinase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0006561; P:proline biosynthetic process; IBA:GO_Central.
DR CDD; cd04242; AAK_G5K_ProB; 1.
DR Gene3D; 2.30.130.10; -; 1.
DR Gene3D; 3.40.1160.10; -; 2.
DR HAMAP; MF_00456; ProB; 1.
DR InterPro; IPR036393; AceGlu_kinase-like_sf.
DR InterPro; IPR001048; Asp/Glu/Uridylate_kinase.
DR InterPro; IPR041739; G5K_ProB.
DR InterPro; IPR001057; Glu/AcGlu_kinase.
DR InterPro; IPR011529; Glu_5kinase.
DR InterPro; IPR005715; Glu_5kinase/COase_Synthase.
DR InterPro; IPR019797; Glutamate_5-kinase_CS.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR Pfam; PF00696; AA_kinase; 1.
DR Pfam; PF01472; PUA; 1.
DR PIRSF; PIRSF000729; GK; 1.
DR PRINTS; PR00474; GLU5KINASE.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF53633; SSF53633; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR01027; proB; 1.
DR PROSITE; PS00902; GLUTAMATE_5_KINASE; 1.
DR PROSITE; PS50890; PUA; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis; ATP-binding; Cytoplasm; Kinase;
KW Nucleotide-binding; Proline biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..423
FT /note="Uncharacterized protein YHR033W"
FT /id="PRO_0000109779"
FT DOMAIN 315..406
FT /note="PUA"
FT BINDING 51
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 138
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 150
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 170..171
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 214..220
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 117
FT /note="N -> D (in Ref. 3; AAU09740)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 423 AA; 46864 MW; C7CBF7B1773F1981 CRC64;
MTKAYTIVIK LGSSSLVDES TKEPKLSIMT LIVETVTNLK RMGHKVIIVS SGGIAVGLDA
LNIPHKPKQL SEVQAIAAVG QGRLIARWNM LFSQYGEQTA QILLTRNDIL RWNQYNNARN
TINELLAMGV IPIVNENDTL SISEIEFGDN DTLSAITAAL VGADFLFLLT DVDCLYTDNP
RTNPDARPIV LVPELSEGLP GVNTSSGSGS EVGTGGMRTK LIAADLASNA GIETIVMKSD
RPEYVPKIVD YIQHHFRPPR HIGNGTQQQF LDLQDTELEQ LRRYDVPMHT KFLANDNKHK
LKNREFWILH GLITKGAIII DENSYDKLLS KDMASLTPNA VIEVRDNFHE LECVDLKIGQ
RLPNGELDIS KPIQSVGCVR SNYTSLELAK IKGLPSEKIH DVLGYSVSEY VAHRENIAFP
PQF
