YHJX_ECOLI
ID YHJX_ECOLI Reviewed; 402 AA.
AC P37662; Q2M7K8;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Uncharacterized MFS-type transporter YhjX {ECO:0000305};
GN Name=yhjX; OrderedLocusNames=b3547, JW3516;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP SUBCELLULAR LOCATION, AND TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [5]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=23222720; DOI=10.1128/jb.02051-12;
RA Fried L., Behr S., Jung K.;
RT "Identification of a target gene and activating stimulus for the YpdA/YpdB
RT histidine kinase/response regulator system in Escherichia coli.";
RL J. Bacteriol. 195:807-815(2013).
RN [6]
RP FUNCTION, INTERACTION WITH BTSS AND YPDA, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=24659770; DOI=10.1128/jb.01554-14;
RA Behr S., Fried L., Jung K.;
RT "Identification of a novel nutrient-sensing histidine kinase/response
RT regulator network in Escherichia coli.";
RL J. Bacteriol. 196:2023-2029(2014).
CC -!- FUNCTION: Part of a nutrient-sensing regulatory network composed of the
CC two-component regulatory systems BtsS/BtsR and YpdA/YpdB, and their
CC respective target proteins, BtsT and YhjX.
CC {ECO:0000269|PubMed:24659770}.
CC -!- SUBUNIT: Interacts with BtsS and YpdA. {ECO:0000269|PubMed:24659770}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:15919996,
CC ECO:0000269|PubMed:23222720}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15919996, ECO:0000269|PubMed:23222720}.
CC -!- INDUCTION: Induced by the two-component regulatory system YpdA/YpdB in
CC response to pyruvate (PubMed:23222720). Regulated at post-
CC transcriptional level by CsrA (PubMed:24659770).
CC {ECO:0000269|PubMed:23222720, ECO:0000269|PubMed:24659770}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; U00039; AAB18524.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76571.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77748.1; -; Genomic_DNA.
DR PIR; S47768; S47768.
DR RefSeq; NP_418003.1; NC_000913.3.
DR RefSeq; WP_000189019.1; NZ_SSZK01000068.1.
DR AlphaFoldDB; P37662; -.
DR SMR; P37662; -.
DR BioGRID; 4260694; 169.
DR DIP; DIP-12395N; -.
DR IntAct; P37662; 1.
DR STRING; 511145.b3547; -.
DR TCDB; 2.A.1.11.3; the major facilitator superfamily (mfs).
DR PaxDb; P37662; -.
DR PRIDE; P37662; -.
DR EnsemblBacteria; AAC76571; AAC76571; b3547.
DR EnsemblBacteria; BAE77748; BAE77748; BAE77748.
DR GeneID; 948066; -.
DR KEGG; ecj:JW3516; -.
DR KEGG; eco:b3547; -.
DR PATRIC; fig|1411691.4.peg.3167; -.
DR EchoBASE; EB2177; -.
DR eggNOG; COG2223; Bacteria.
DR HOGENOM; CLU_001265_59_7_6; -.
DR InParanoid; P37662; -.
DR OMA; WVLASHQ; -.
DR PhylomeDB; P37662; -.
DR BioCyc; EcoCyc:YHJX-MON; -.
DR PRO; PR:P37662; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0031669; P:cellular response to nutrient levels; IEP:EcoCyc.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004741; Oxa_For_antiport_fam_transptr.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00890; 2A0111; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..402
FT /note="Uncharacterized MFS-type transporter YhjX"
FT /id="PRO_0000169585"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..43
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..72
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..95
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..99
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 123..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..168
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..216
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..250
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 251..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 274..279
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 280..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..306
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 307..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..367
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 368..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..402
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 402 AA; 42951 MW; FD2A2189B5873688 CRC64;
MTPSNYQRTR WLTLIGTIIT QFALGSVYTW SLFNGALSAK LDAPVSQVAF SFGLLSLGLA
ISSSVAGKLQ ERFGVKRVTM ASGILLGLGF FLTAHSDNLM MLWLSAGVLV GLADGAGYLL
TLSNCVKWFP ERKGLISAFA IGSYGLGSLG FKFIDTQLLE TVGLEKTFVI WGAIALLMIV
FGATLMKDAP KQEVKTSNGV VEKDYTLAES MRKPQYWMLA VMFLTACMSG LYVIGVAKDI
AQSLAHLDVV SAANAVTVIS IANLSGRLVL GILSDKIARI RVITIGQVIS LVGMAALLFA
PLNAVTFFAA IACVAFNFGG TITVFPSLVS EFFGLNNLAK NYGVIYLGFG IGSICGSIIA
SLFGGFYVTF YVIFALLILS LALSTTIRQP EQKMLREAHG SL
