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YI13_SCHPO
ID   YI13_SCHPO              Reviewed;         259 AA.
AC   Q9P7B4;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 120.
DE   RecName: Full=NADP-dependent 3-hydroxy acid dehydrogenase {ECO:0000250|UniProtKB:Q05016};
DE   AltName: Full=L-allo-threonine dehydrogenase {ECO:0000250|UniProtKB:Q05016};
DE            EC=1.1.1.381 {ECO:0000250|UniProtKB:Q05016};
GN   ORFNames=SPAC521.03;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
RN   [3]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-42 AND THR-43, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=18257517; DOI=10.1021/pr7006335;
RA   Wilson-Grady J.T., Villen J., Gygi S.P.;
RT   "Phosphoproteome analysis of fission yeast.";
RL   J. Proteome Res. 7:1088-1097(2008).
CC   -!- FUNCTION: NADP-dependent dehydrogenase with broad substrate specificity
CC       acting on 3-hydroxy acids. Catalyzes the NADP-dependent oxidation of L-
CC       allo-threonine to L-2-amino-3-keto-butyrate, which is spontaneously
CC       decarboxylated into aminoacetone. Also acts on D-threonine, L-serine,
CC       D-serine, D-3-hydroxyisobutyrate, L-3-hydroxyisobutyrate, D-glycerate
CC       and L-glycerate. {ECO:0000250|UniProtKB:Q05016}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=L-allo-threonine + NADP(+) = aminoacetone + CO2 + NADPH;
CC         Xref=Rhea:RHEA:43524, ChEBI:CHEBI:16526, ChEBI:CHEBI:57783,
CC         ChEBI:CHEBI:58320, ChEBI:CHEBI:58349, ChEBI:CHEBI:58585;
CC         EC=1.1.1.381; Evidence={ECO:0000250|UniProtKB:Q05016};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:Q05016}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC       {ECO:0000250|UniProtKB:Q05016}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; CU329670; CAB86467.1; -; Genomic_DNA.
DR   RefSeq; NP_593098.1; NM_001018495.2.
DR   AlphaFoldDB; Q9P7B4; -.
DR   SMR; Q9P7B4; -.
DR   BioGRID; 279881; 10.
DR   STRING; 4896.SPAC521.03.1; -.
DR   iPTMnet; Q9P7B4; -.
DR   MaxQB; Q9P7B4; -.
DR   PaxDb; Q9P7B4; -.
DR   PRIDE; Q9P7B4; -.
DR   EnsemblFungi; SPAC521.03.1; SPAC521.03.1:pep; SPAC521.03.
DR   GeneID; 2543461; -.
DR   KEGG; spo:SPAC521.03; -.
DR   PomBase; SPAC521.03; -.
DR   VEuPathDB; FungiDB:SPAC521.03; -.
DR   eggNOG; KOG1205; Eukaryota.
DR   HOGENOM; CLU_010194_2_10_1; -.
DR   InParanoid; Q9P7B4; -.
DR   OMA; WRWMWET; -.
DR   PhylomeDB; Q9P7B4; -.
DR   PRO; PR:Q9P7B4; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; NAD; NADP; Nucleus; Oxidoreductase; Phosphoprotein;
KW   Reference proteome.
FT   CHAIN           1..259
FT                   /note="NADP-dependent 3-hydroxy acid dehydrogenase"
FT                   /id="PRO_0000372617"
FT   ACT_SITE        158
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         13..18
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   BINDING         39..40
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   BINDING         65..66
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   BINDING         92
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   BINDING         145
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         158
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   BINDING         162
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   BINDING         188..195
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250|UniProtKB:Q05016"
FT   MOD_RES         42
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
FT   MOD_RES         43
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18257517"
SQ   SEQUENCE   259 AA;  28149 MW;  EA8FBCBF231FCC08 CRC64;
     MSRLDGKTIL ITGASSGIGK STAFEIAKVA KVKLILAARR FSTVEEIAKE LESKYEVSVL
     PLKLDVSDLK SIPGVIESLP KEFADIDVLI NNAGLALGTD KVIDLNIDDA VTMITTNVLG
     MMAMTRAVLP IFYSKNKGDI LNVGSIAGRE SYVGGSVYCS TKSALAQFTS ALRKETIDTR
     IRIMEVDPGL VETEFSVVRF HGDKQKADNV YKNSEPLTPE DIAEVILFAL TRRENVVIAD
     TLVFPSHQGG ANHVYRKQA
 
 
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