ID   YIAJ_ECOLI              Reviewed;         282 AA.
AC   P37671; Q2M7N7;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   03-AUG-2022, entry version 158.
DE   RecName: Full=DNA-binding transcriptional repressor YiaJ;
DE   AltName: Full=YiaKLMNOPQRS operon repressor;
DE            Short=YiaK-S operon repressor;
GN   Name=yiaJ; OrderedLocusNames=b3574, JW3546;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA   Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT   "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT   from 76.0 to 81.5 minutes.";
RL   Nucleic Acids Res. 22:2576-2586(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, AND INDUCTION.
RC   STRAIN=K12;
RX   PubMed=10913096; DOI=10.1128/jb.182.16.4617-4624.2000;
RA   Ibanez E., Campos E., Baldoma L., Aguilar J., Badia J.;
RT   "Regulation of expression of the yiaKLMNOPQRS operon for carbohydrate
RT   utilization in Escherichia coli: involvement of the main transcriptional
RT   factors.";
RL   J. Bacteriol. 182:4617-4624(2000).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 94-282.
RG   Midwest center for structural genomics (MCSG);
RT   "The crystal structure of transcriptional regulator YiaJ.";
RL   Submitted (MAR-2005) to the PDB data bank.
CC   -!- FUNCTION: Negatively controls the transcription of the yiaKLMNOPQRS
CC       operon, which may be involved in the utilization of 2,3-diketo-L-
CC       gulonate. {ECO:0000269|PubMed:10913096}.
CC   -!- INDUCTION: Expression of yiaJ is autogenously regulated and reduced by
CC       the binding of CRP-cAMP to the CRP site 1 of the YiaK-S promoter.
CC       {ECO:0000269|PubMed:10913096}.
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DR   EMBL; U00039; AAB18551.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76598.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77719.1; -; Genomic_DNA.
DR   PIR; S47795; S47795.
DR   RefSeq; NP_418031.1; NC_000913.3.
DR   RefSeq; WP_000514240.1; NZ_SSZK01000041.1.
DR   PDB; 1YSQ; X-ray; 1.75 A; A=94-282.
DR   PDBsum; 1YSQ; -.
DR   AlphaFoldDB; P37671; -.
DR   SMR; P37671; -.
DR   BioGRID; 4259370; 4.
DR   BioGRID; 852392; 2.
DR   IntAct; P37671; 3.
DR   STRING; 511145.b3574; -.
DR   jPOST; P37671; -.
DR   PaxDb; P37671; -.
DR   PRIDE; P37671; -.
DR   EnsemblBacteria; AAC76598; AAC76598; b3574.
DR   EnsemblBacteria; BAE77719; BAE77719; BAE77719.
DR   GeneID; 948084; -.
DR   KEGG; ecj:JW3546; -.
DR   KEGG; eco:b3574; -.
DR   PATRIC; fig|1411691.4.peg.3138; -.
DR   EchoBASE; EB2186; -.
DR   eggNOG; COG1414; Bacteria.
DR   HOGENOM; CLU_062618_5_5_6; -.
DR   InParanoid; P37671; -.
DR   OMA; RYPVRMY; -.
DR   PhylomeDB; P37671; -.
DR   BioCyc; EcoCyc:EG12278-MON; -.
DR   EvolutionaryTrace; P37671; -.
DR   PRO; PR:P37671; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 3.30.450.40; -; 1.
DR   InterPro; IPR029016; GAF-like_dom_sf.
DR   InterPro; IPR014757; Tscrpt_reg_IclR_C.
DR   InterPro; IPR005471; Tscrpt_reg_IclR_N.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF09339; HTH_IclR; 1.
DR   Pfam; PF01614; IclR; 1.
DR   SMART; SM00346; HTH_ICLR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS51077; HTH_ICLR; 1.
DR   PROSITE; PS51078; ICLR_ED; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..282
FT                   /note="DNA-binding transcriptional repressor YiaJ"
FT                   /id="PRO_0000201771"
FT   DOMAIN          23..85
FT                   /note="HTH iclR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   DOMAIN          100..272
FT                   /note="IclR-ED"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00394"
FT   DNA_BIND        45..64
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00393"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   HELIX           98..115
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   STRAND          117..125
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   STRAND          128..135
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   STRAND          151..153
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   HELIX           154..156
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   HELIX           158..165
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   HELIX           169..178
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   TURN            179..182
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   HELIX           194..207
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   STRAND          210..217
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   STRAND          220..227
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   STRAND          235..243
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   HELIX           244..250
FT                   /evidence="ECO:0007829|PDB:1YSQ"
FT   HELIX           252..272
FT                   /evidence="ECO:0007829|PDB:1YSQ"
SQ   SEQUENCE   282 AA;  31067 MW;  22E887B6672B958F CRC64;
     MGKEVMGKKE NEMAQEKERP AGSQSLFRGL MLIEILSNYP NGCPLAHLSE LAGLNKSTVH
     RLLQGLQSCG YVTTAPAAGS YRLTTKFIAV GQKALSSLNI IHIAAPHLEA LNIATGETIN
     FSSREDDHAI LIYKLEPTTG MLRTRAYIGQ HMPLYCSAMG KIYMAFGHPD YVKSYWESHQ
     HEIQPLTRNT ITELPAMFDE LAHIRESGAA MDREENELGV SCIAVPVFDI HGRVPYAVSI
     SLSTSRLKQV GEKNLLKPLR ETAQAISNEL GFTVRDDLGA IT