YIAM_ECOLI
ID YIAM_ECOLI Reviewed; 157 AA.
AC P37674; Q2M7P0;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=2,3-diketo-L-gulonate TRAP transporter small permease protein YiaM;
GN Name=yiaM; OrderedLocusNames=b3577, JW3549;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8041620; DOI=10.1093/nar/22.13.2576;
RA Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
RT "Analysis of the Escherichia coli genome. V. DNA sequence of the region
RT from 76.0 to 81.5 minutes.";
RL Nucleic Acids Res. 22:2576-2586(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP PRELIMINARY FUNCTION.
RC STRAIN=K12;
RX PubMed=14668138; DOI=10.1080/09687680310001607369;
RA Plantinga T.H., van der Does C., Badia J., Aguilar J., Konings W.N.,
RA Driessen A.J.M.;
RT "Functional characterization of the Escherichia coli K-12 yiaMNO transport
RT protein genes.";
RL Mol. Membr. Biol. 21:51-57(2004).
RN [5]
RP DELETION STUDIES.
RC STRAIN=K12;
RX PubMed=15870475; DOI=10.1099/mic.0.27851-0;
RA Plantinga T.H., van der Does C., Tomkiewicz D., van Keulen G.,
RA Konings W.N., Driessen A.J.M.;
RT "Deletion of the yiaMNO transporter genes affects the growth
RT characteristics of Escherichia coli K-12.";
RL Microbiology 151:1683-1689(2005).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [7]
RP FUNCTION.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16385129; DOI=10.1099/mic.0.28334-0;
RA Thomas G.H., Southworth T., Leon-Kempis M.R., Leech A., Kelly D.J.;
RT "Novel ligands for the extracellular solute receptors of two bacterial TRAP
RT transporters.";
RL Microbiology 152:187-198(2006).
CC -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC transport system YiaMNO involved in the uptake of 2,3-diketo-L-
CC gulonate. {ECO:0000269|PubMed:16385129}.
CC -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC protein YiaO, and the two transmembrane proteins YiaM and YiaN.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the TRAP transporter small permease family.
CC {ECO:0000305}.
CC -!- CAUTION: Was originally proposed to be a subunit from an L-xylulose
CC uptake system, but PubMed:16385129 shows that it does not bind L- or D-
CC xylulose. {ECO:0000305|PubMed:14668138}.
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DR EMBL; U00039; AAB18554.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76601.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77716.1; -; Genomic_DNA.
DR PIR; S47798; S47798.
DR RefSeq; NP_418034.1; NC_000913.3.
DR RefSeq; WP_000721664.1; NZ_STEB01000018.1.
DR AlphaFoldDB; P37674; -.
DR BioGRID; 4262548; 5.
DR ComplexPortal; CPX-4681; YiaMNO tripartite ATP-independent periplasmic transporter complex.
DR STRING; 511145.b3577; -.
DR TCDB; 2.A.56.1.2; the tripartite atp-independent periplasmic transporter (trap-t) family.
DR PaxDb; P37674; -.
DR EnsemblBacteria; AAC76601; AAC76601; b3577.
DR EnsemblBacteria; BAE77716; BAE77716; BAE77716.
DR GeneID; 66672534; -.
DR GeneID; 948093; -.
DR KEGG; ecj:JW3549; -.
DR KEGG; eco:b3577; -.
DR PATRIC; fig|1411691.4.peg.3135; -.
DR EchoBASE; EB2189; -.
DR eggNOG; COG3090; Bacteria.
DR HOGENOM; CLU_086356_9_0_6; -.
DR InParanoid; P37674; -.
DR OMA; MLFCCYL; -.
DR PhylomeDB; P37674; -.
DR BioCyc; EcoCyc:MON0-422; -.
DR BioCyc; MetaCyc:MON0-422; -.
DR PRO; PR:P37674; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IC:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0031317; C:tripartite ATP-independent periplasmic transporter complex; IC:ComplexPortal.
DR GO; GO:0015144; F:carbohydrate transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015740; P:C4-dicarboxylate transport; IBA:GO_Central.
DR GO; GO:0034219; P:carbohydrate transmembrane transport; IDA:EcoCyc.
DR GO; GO:1902075; P:cellular response to salt; IC:ComplexPortal.
DR GO; GO:1900190; P:regulation of single-species biofilm formation; IC:ComplexPortal.
DR InterPro; IPR007387; TRAP_DctQ.
DR PANTHER; PTHR35011; PTHR35011; 1.
DR Pfam; PF04290; DctQ; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..157
FT /note="2,3-diketo-L-gulonate TRAP transporter small
FT permease protein YiaM"
FT /id="PRO_0000169595"
FT TOPO_DOM 1..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..43
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..66
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 67..78
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..98
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 99..112
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..157
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 157 AA; 17516 MW; E8839A3FCEB2BEDA CRC64;
MKKILEAILA INLAVLSCIV FINIILRYGF QTSILSVDEL SRYLFVWLTF IGAIVAFMDN
AHVQVTFLVE KLSPAWQRRV ALVTHSLILF ICGALAWGAT LKTIQDWSDY SPILGLPIGL
MYAACLPTSL VIAFFELRHL YQLITRSNSL TSPPQGA
