YIKE_SCHPO
ID YIKE_SCHPO Reviewed; 668 AA.
AC Q8TFH0;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-APR-2012, sequence version 2.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Probable 6-phosphofructo-2-kinase PB17E12.14c;
DE EC=2.7.1.105;
GN ORFNames=SPAPB17E12.14c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
RN [3]
RP REVISION OF GENE MODEL, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=21270388; DOI=10.1534/genetics.110.123497;
RA Bitton D.A., Wood V., Scutt P.J., Grallert A., Yates T., Smith D.L.,
RA Hagan I.M., Miller C.J.;
RT "Augmented annotation of the Schizosaccharomyces pombe genome reveals
RT additional genes required for growth and viability.";
RL Genetics 187:1207-1217(2011).
CC -!- FUNCTION: Synthesis of fructose 2,6-bisphosphate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-
CC bisphosphate + H(+); Xref=Rhea:RHEA:15653, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:57634, ChEBI:CHEBI:58579,
CC ChEBI:CHEBI:456216; EC=2.7.1.105;
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DR EMBL; CU329670; CAD27507.2; -; Genomic_DNA.
DR RefSeq; NP_001018229.2; NM_001018709.2.
DR AlphaFoldDB; Q8TFH0; -.
DR SMR; Q8TFH0; -.
DR BioGRID; 280454; 2.
DR STRING; 4896.SPAPB17E12.14c.1; -.
DR iPTMnet; Q8TFH0; -.
DR MaxQB; Q8TFH0; -.
DR PaxDb; Q8TFH0; -.
DR PRIDE; Q8TFH0; -.
DR EnsemblFungi; SPAPB17E12.14c.1; SPAPB17E12.14c.1:pep; SPAPB17E12.14c.
DR GeneID; 3361378; -.
DR KEGG; spo:SPAPB17E12.14c; -.
DR PomBase; SPAPB17E12.14c; -.
DR VEuPathDB; FungiDB:SPAPB17E12.14c; -.
DR eggNOG; KOG0234; Eukaryota.
DR HOGENOM; CLU_006383_3_1_1; -.
DR InParanoid; Q8TFH0; -.
DR OMA; ITDHVLI; -.
DR PRO; PR:Q8TFH0; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003873; F:6-phosphofructo-2-kinase activity; ISO:PomBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006003; P:fructose 2,6-bisphosphate metabolic process; ISO:PomBase.
DR GO; GO:0006000; P:fructose metabolic process; IEA:InterPro.
DR CDD; cd07067; HP_PGM_like; 1.
DR Gene3D; 3.40.50.1240; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003094; 6Pfruct_kin.
DR InterPro; IPR013079; 6Phosfructo_kin.
DR InterPro; IPR013078; His_Pase_superF_clade-1.
DR InterPro; IPR029033; His_PPase_superfam.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001345; PG/BPGM_mutase_AS.
DR PANTHER; PTHR10606; PTHR10606; 1.
DR Pfam; PF01591; 6PF2K; 1.
DR Pfam; PF00300; His_Phos_1; 1.
DR PRINTS; PR00991; 6PFRUCTKNASE.
DR SMART; SM00855; PGAM; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF53254; SSF53254; 1.
DR PROSITE; PS00175; PG_MUTASE; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..668
FT /note="Probable 6-phosphofructo-2-kinase PB17E12.14c"
FT /id="PRO_0000318494"
FT REGION 1..105
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 136..185
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 281
FT /evidence="ECO:0000255"
FT ACT_SITE 312
FT /evidence="ECO:0000255"
FT ACT_SITE 540
FT /evidence="ECO:0000255"
FT ACT_SITE 608
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 197..204
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT BINDING 346
FT /ligand="beta-D-fructose 6-phosphate"
FT /ligand_id="ChEBI:CHEBI:57634"
FT /evidence="ECO:0000250"
SQ SEQUENCE 668 AA; 76393 MW; BA1228BA08951A78 CRC64;
MSNNNNKDDS ELQSRTHYYN PDRTAIPVKD DVDQLDKFGS PKLSANDHSF TNTDSVTSED
RPFSSPVSTL TSNSANFSDS SLQNSPVHPI PSPALSPTLN LGSRPDVKRV SSGGYFALPK
QLTSIRQTHR PMSQQHRVPS FHPASDTAPP SPIPFHHHLR KKGSSMSIPG QTSIVSSNNG
SEATNPPEEK LAIIMVGLPA RGKSYIVNKL VRYYNWLQYN CKAFNVGNFR RKHASHSHDD
ASFFDPSNEE ASKLRESFAM DTLDALLQWF EEEDGVVGIF DATNSTSKRR KAIVDHLSKV
PYVTTLFIES ICNDEQLIAA NMRMKLVGPD YKDLNPEQSL RDFQERVRMY ERKYEPLGKS
EEDLNLRYIK VINVGKKVVA FNIRGFLAGQ AVFFLLNFNL SPRQIWVTRH GESVDNVRGR
IGGNAELTPL GRQFSEDLAL FIDEKRDEFQ ERLYNDYSKE SHLLQHGSHS FNGKQFETSF
NCLTPSENTV NDPQVLDPED EERLEKPYSV WTSMMQRSIQ TAAYFDEEQY DIKAMRMLNE
ICSGICDGLT YEEIKSIYPK EYEARKLDKL NYRYPGSGGE SYLDVIYRLQ SVIVEIERMK
HHVLVIGHRV ITRIIIAYFL GCRREDIAYL NVPLHTVYCI EPQPYGTDFY QYNYDPNTRK
FSRVPFSL
