ID   CBIG_METJA              Reviewed;         323 AA.
AC   Q58544;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Probable cobalt-precorrin-5A hydrolase;
DE            EC=3.7.1.12;
GN   Name=cbiG; OrderedLocusNames=MJ1144;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: Catalyzes the hydrolysis of the ring A acetate delta-lactone
CC       of cobalt-precorrin-5A resulting in the loss of the C-20 carbon and its
CC       attached methyl group in the form of acetaldehyde. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-5A + H2O = acetaldehyde + Co-precorrin-5B + H(+);
CC         Xref=Rhea:RHEA:26281, ChEBI:CHEBI:15343, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:60062, ChEBI:CHEBI:60063; EC=3.7.1.12;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 5/10.
CC   -!- SIMILARITY: Belongs to the CbiG family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99144.1; -; Genomic_DNA.
DR   PIR; G64442; G64442.
DR   AlphaFoldDB; Q58544; -.
DR   SMR; Q58544; -.
DR   STRING; 243232.MJ_1144; -.
DR   PRIDE; Q58544; -.
DR   EnsemblBacteria; AAB99144; AAB99144; MJ_1144.
DR   KEGG; mja:MJ_1144; -.
DR   eggNOG; arCOG00651; Archaea.
DR   HOGENOM; CLU_028397_0_0_2; -.
DR   InParanoid; Q58544; -.
DR   OMA; GIGCNRG; -.
DR   PhylomeDB; Q58544; -.
DR   UniPathway; UPA00148; UER00561.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0043779; F:cobalt-precorrin-5A acetaldehyde-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.30.420.180; -; 1.
DR   InterPro; IPR021744; CbiG_N.
DR   InterPro; IPR002750; CobE/GbiG_C.
DR   InterPro; IPR036518; CobE/GbiG_C_sf.
DR   InterPro; IPR038029; GbiG_N_sf.
DR   Pfam; PF01890; CbiG_C; 1.
DR   Pfam; PF11760; CbiG_N; 1.
DR   SUPFAM; SSF159664; SSF159664; 1.
DR   SUPFAM; SSF159672; SSF159672; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; Hydrolase; Reference proteome.
FT   CHAIN           1..323
FT                   /note="Probable cobalt-precorrin-5A hydrolase"
FT                   /id="PRO_0000107187"
SQ   SEQUENCE   323 AA;  37500 MW;  E6476F3F39DDBCE4 CRC64;
     MMIKIVYITK RGKKIAEEIK DVLDYYHYDN KVEPIKDFKI ERNEGGFIFI MATGIVLRKF
     LDEIKNDKFK DPFVIICNEN KELIPILSNH LGGGNYFSKL IANNINGRVI FTTATDVNGK
     VGIDELSKML FLETPKRKHI LDINKKILEE DVSLTLPKYW KLRNLNGYKI SYHDKYEVVV
     DDSIRLKPLK IAVGLGARKG IERYKVYWAV KKALFLRNIP VWRVDAFATI EDKKHERGIL
     ETVNKFKKPL IIFKREEINE IYEKIDLEKS EFVYKHLGVY GVSEPASILA VKKLTNKDFD
     SIKLILKKFK RNGVTVAIAT ENL