ID   CBIJ_METMI              Reviewed;         248 AA.
AC   Q977V1;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 2.
DT   25-MAY-2022, entry version 38.
DE   RecName: Full=Cobalt-precorrin-6A reductase;
DE            EC=1.3.1.106;
GN   Name=cbiJ;
OS   Methanococcus maripaludis (Methanococcus deltae).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=39152;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=ATCC 43000 / DSM 2067 / JCM 10722 / JJ;
RX   PubMed=16243778; DOI=10.1155/2005/903614;
RA   Kim W., Major T.A., Whitman W.B.;
RT   "Role of the precorrin 6-X reductase gene in cobamide biosynthesis in
RT   Methanococcus maripaludis.";
RL   Archaea 1:375-384(2005).
CC   -!- FUNCTION: Catalyzes the reduction of the macrocycle of cobalt-
CC       precorrin-6A to cobalt-precorrin-6B. {ECO:0000269|PubMed:16243778}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-6B + NAD(+) = Co-precorrin-6A + H(+) + NADH;
CC         Xref=Rhea:RHEA:15625, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60064, ChEBI:CHEBI:72780;
CC         EC=1.3.1.106;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin-6x reductase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00356}.
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DR   EMBL; AF402610; AAK94917.2; -; Genomic_DNA.
DR   AlphaFoldDB; Q977V1; -.
DR   SMR; Q977V1; -.
DR   KEGG; ag:AAK94917; -.
DR   BRENDA; 1.3.1.106; 3262.
DR   BRENDA; 1.3.1.54; 3262.
DR   UniPathway; UPA00148; UER00228.
DR   GO; GO:0016994; F:precorrin-6A reductase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003723; Precorrin-6x_reduct.
DR   PANTHER; PTHR36925; PTHR36925; 1.
DR   Pfam; PF02571; CbiJ; 1.
DR   TIGRFAMs; TIGR00715; precor6x_red; 1.
DR   PROSITE; PS51014; COBK_CBIJ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; NAD; Oxidoreductase.
FT   CHAIN           1..248
FT                   /note="Cobalt-precorrin-6A reductase"
FT                   /id="PRO_0000429643"
SQ   SEQUENCE   248 AA;  27999 MW;  1428C806CA30006D CRC64;
     MNIWIRGGTS DANNISKEIK RNFKDSFLIL TTTTDFGGKI AENFADLVIS EKMTYDNLKK
     TLLDKKIDVF IDATHPFATH ASETGIKISK ELNIPYIRYE RPSEKFKNAF YVENYEEAAK
     LALKISKKNI FYMSGIKNLK NVSEIIPIEK LIVRILPTSV PEALKILPSK NIVAMQGVFS
     ENLNKELIID YNCDVIITKD SGKSGGLYEK VSGATLAGAK PIIIKRPEIN YPLKFEKIVE
     IVNYLKNV