ID   CBIJ_METTH              Reviewed;         302 AA.
AC   O27083;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Cobalt-precorrin-6A reductase;
DE            EC=1.3.1.106;
GN   Name=cbiJ; Synonyms=cobK; OrderedLocusNames=MTH_1002;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Catalyzes the reduction of the macrocycle of cobalt-
CC       precorrin-6A to cobalt-precorrin-6B. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-6B + NAD(+) = Co-precorrin-6A + H(+) + NADH;
CC         Xref=Rhea:RHEA:15625, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60064, ChEBI:CHEBI:72780;
CC         EC=1.3.1.106;
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin-6x reductase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00356}.
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DR   EMBL; AE000666; AAB85498.1; -; Genomic_DNA.
DR   PIR; F69000; F69000.
DR   RefSeq; WP_010876633.1; NC_000916.1.
DR   AlphaFoldDB; O27083; -.
DR   SMR; O27083; -.
DR   STRING; 187420.MTH_1002; -.
DR   EnsemblBacteria; AAB85498; AAB85498; MTH_1002.
DR   GeneID; 1471410; -.
DR   KEGG; mth:MTH_1002; -.
DR   PATRIC; fig|187420.15.peg.985; -.
DR   HOGENOM; CLU_068627_0_0_2; -.
DR   OMA; THPFAIN; -.
DR   UniPathway; UPA00148; UER00228.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0016994; F:precorrin-6A reductase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003723; Precorrin-6x_reduct.
DR   PANTHER; PTHR36925; PTHR36925; 1.
DR   Pfam; PF02571; CbiJ; 1.
DR   TIGRFAMs; TIGR00715; precor6x_red; 1.
DR   PROSITE; PS51014; COBK_CBIJ; 1.
PE   3: Inferred from homology;
KW   Cobalamin biosynthesis; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..302
FT                   /note="Cobalt-precorrin-6A reductase"
FT                   /id="PRO_0000135918"
FT   REGION          1..37
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        21..37
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   302 AA;  32673 MW;  F0BCDBC37DEDF3C5 CRC64;
     MQTPEIKEGT EQYLWRRKTM NPGDKGVKRK GSDRQREKMS VIVMAGTEDA RRIISRLSGM
     PWVEVTATAT TEHGSDLAEK SGASRTVTGA LDSDGLRELM ADLDACILID ATHPFAAQAT
     ENALRACRET GTIYVRFERP EVIPDGVIRV GSFREAGEVA SSLIGDGEVV MHLAGVSTLG
     DVLRSLEPER VAVRVLPSTS SIEKCLQLGV PPSHIIAMQG RFSAEMNLAL LREYRAGAVI
     TKESGETGGL PEKVEAASEL GIPVILVERP EVNLEGEAVF GNINDLMDHV LKILRDMGQP
     GD