YJ66_YEAST
ID YJ66_YEAST Reviewed; 282 AA.
AC P47137; D6VWR5;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Uncharacterized oxidoreductase YJR096W;
DE EC=1.-.-.-;
GN OrderedLocusNames=YJR096W; ORFNames=J1926;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- INTERACTION:
CC P47137; P33203: PRP40; NbExp=2; IntAct=EBI-25572, EBI-701;
CC P47137; P39940: RSP5; NbExp=3; IntAct=EBI-25572, EBI-16219;
CC -!- MISCELLANEOUS: Present with 6960 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR EMBL; Z49596; CAA89626.1; -; Genomic_DNA.
DR EMBL; AY558257; AAS56583.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08881.1; -; Genomic_DNA.
DR PIR; S57117; S57117.
DR RefSeq; NP_012630.1; NM_001181754.1.
DR AlphaFoldDB; P47137; -.
DR SMR; P47137; -.
DR BioGRID; 33851; 89.
DR IntAct; P47137; 16.
DR MINT; P47137; -.
DR STRING; 4932.YJR096W; -.
DR MaxQB; P47137; -.
DR PaxDb; P47137; -.
DR PRIDE; P47137; -.
DR EnsemblFungi; YJR096W_mRNA; YJR096W; YJR096W.
DR GeneID; 853559; -.
DR KEGG; sce:YJR096W; -.
DR SGD; S000003857; YJR096W.
DR VEuPathDB; FungiDB:YJR096W; -.
DR eggNOG; KOG1577; Eukaryota.
DR HOGENOM; CLU_023205_0_1_1; -.
DR InParanoid; P47137; -.
DR OMA; PWCMRQE; -.
DR BioCyc; YEAST:G3O-31724-MON; -.
DR PRO; PR:P47137; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P47137; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IDA:SGD.
DR GO; GO:0004033; F:aldo-keto reductase (NADP) activity; IDA:SGD.
DR GO; GO:0019568; P:arabinose catabolic process; IDA:SGD.
DR GO; GO:0034599; P:cellular response to oxidative stress; IGI:SGD.
DR GO; GO:0042843; P:D-xylose catabolic process; IMP:SGD.
DR Gene3D; 3.20.20.100; -; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; PTHR43827; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; SSF51430; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 1: Evidence at protein level;
KW Oxidoreductase; Reference proteome.
FT CHAIN 1..282
FT /note="Uncharacterized oxidoreductase YJR096W"
FT /id="PRO_0000124679"
FT ACT_SITE 50
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 115
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT SITE 81
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000250"
SQ SEQUENCE 282 AA; 32344 MW; 692F14FB0B504B95 CRC64;
MVPKFYKLSN GFKIPSIALG TYDIPRSQTA EIVYEGVKCG YRHFDTAVLY GNEKEVGDGI
IKWLNEDPGN HKREEIFYTT KLWNSQNGYK RAKAAIRQCL NEVSGLQYID LLLIHSPLEG
SKLRLETWRA MQEAVDEGLV KSIGVSNYGK KHIDELLNWP ELKHKPVVNQ IEISPWIMRQ
ELADYCKSKG LVVEAFAPLC HGYKMTNPDL LKVCKEVDRN PGQVLIRWSL QHGYLPLPKT
KTVKRLEGNL AAYNFELSDE QMKFLDHPDA YEPTDWECTD AP
