ID   CBIJ_PRIMG              Reviewed;         255 AA.
AC   O87691;
DT   09-JUL-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Cobalt-precorrin-6A reductase;
DE            EC=1.3.1.106;
GN   Name=cbiJ;
OS   Priestia megaterium (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=1404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 509 / CCM 1464 / NBRC 12109;
RX   PubMed=9742225; DOI=10.1042/bj3350159;
RA   Raux E., Lanois A., Warren M.J., Rambach A., Thermes C.;
RT   "Cobalamin (vitamin B12) biosynthesis: identification and characterization
RT   of a Bacillus megaterium cobI operon.";
RL   Biochem. J. 335:159-166(1998).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=23922391; DOI=10.1073/pnas.1308098110;
RA   Moore S.J., Lawrence A.D., Biedendieck R., Deery E., Frank S., Howard M.J.,
RA   Rigby S.E., Warren M.J.;
RT   "Elucidation of the anaerobic pathway for the corrin component of cobalamin
RT   (vitamin B12).";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:14906-14911(2013).
CC   -!- FUNCTION: Catalyzes the reduction of the macrocycle of cobalt-
CC       precorrin-6A to cobalt-precorrin-6B. {ECO:0000269|PubMed:23922391}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Co-precorrin-6B + NAD(+) = Co-precorrin-6A + H(+) + NADH;
CC         Xref=Rhea:RHEA:15625, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:60064, ChEBI:CHEBI:72780;
CC         EC=1.3.1.106; Evidence={ECO:0000269|PubMed:23922391};
CC   -!- PATHWAY: Cofactor biosynthesis; adenosylcobalamin biosynthesis;
CC       cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route):
CC       step 7/10.
CC   -!- SIMILARITY: Belongs to the precorrin-6x reductase family.
CC       {ECO:0000255|PROSITE-ProRule:PRU00356}.
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DR   EMBL; AJ000758; CAA04309.1; -; Genomic_DNA.
DR   PIR; T44685; T44685.
DR   AlphaFoldDB; O87691; -.
DR   SMR; O87691; -.
DR   BioCyc; MetaCyc:MON-18418; -.
DR   UniPathway; UPA00148; UER00228.
DR   GO; GO:0016994; F:precorrin-6A reductase activity; IEA:InterPro.
DR   GO; GO:0009236; P:cobalamin biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003723; Precorrin-6x_reduct.
DR   PANTHER; PTHR36925; PTHR36925; 1.
DR   Pfam; PF02571; CbiJ; 1.
DR   TIGRFAMs; TIGR00715; precor6x_red; 1.
DR   PROSITE; PS51014; COBK_CBIJ; 1.
PE   1: Evidence at protein level;
KW   Cobalamin biosynthesis; NAD; Oxidoreductase.
FT   CHAIN           1..255
FT                   /note="Cobalt-precorrin-6A reductase"
FT                   /id="PRO_0000429642"
SQ   SEQUENCE   255 AA;  27994 MW;  370E2B16FAD55837 CRC64;
     MILLLAGTSD ARALAVQVKK AGYDVTATVV TDNAAIELQR AEVKVKIGRL TKEDMTDFIN
     EHGVKAIVDA SHPFAEEASK NAIGAAAETA IPYIRYERAS QAFTYDNMTM VSTYEEAAEV
     AAEKKGVIML TTGSKTLQVF TEKLLPLSDV RLVARMLPRL DNMEKCQQLG LPQKNIIAIQ
     GPFTKEFDRA LYKQYGVTVM VTKESGKVGS VDKKVEAAKE LGLDIIMIGR PKIEYGTVYS
     TFEEVVHALV NQTRS